림프구 침윤을 보이는 피부질환에서 Ki - 67 항원의 발현

양성규,조광현,김철우 (Seong Gyu Yang,Kwang Hyun Cho and Chul Woo Kim) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Background: A number of reports have stressed the usefulness of cell kinetic estimations as an aid in the prognostic and diagnostic classification of malignant lymphoma. But very limited information is available concerning cell cycle distirbutions in cutaneous lymphoid infiltrate, although a number of these conditions present tremendous problems in diagnosis and management. Objective : The purpose of this study is to evaluate the proliferative activities in various cutaneous lymphoid infiltrates and its usefulness in diagnosis or foretelling a prognosis. Methods : Fifty two arehival biopsy specimens were studies. These included cases of peripheral T-cell lymphoma, lichen planus, lupus erythematosus, polymorphous light eruptions, and pseudolymphomas such as insect bite, lymphomatoid papulosis, and other lymphocytoma cutis. Ki-67 was identified by immunoperoxidase technique using MIB1, a monoclonal antibody that recognizes a fixation and embedding resistant epitope on the Ki-67 protein. Results : Higher Ki-67 positive rates were observed in high grade angiocentric T-cell lymphomas, which was helpful in differentiating from benign disease. In two cases of lymphomas which Ki-67 positive rate were more than 80 percent despite of minimal lymphoid infiltrate in skin biopsy specimen, evaluation of Ki-67 expression was profoundly helpful in diagnosing them as malignancies. Conclusion : Use of this technique was useful in evaluation of proliferative activity of cutaneous lymphoid infiltrate. In some cases, use of this technique may aid us in differential diagnosis of malignant lymphoma from benign chronic inflammatory disease of the skin. (Kor J Dermatol 1996;34(2): 219-226)

Lofgren 증후군

양성규 ( Seong Gyu Yang ),서대헌 ( Dae Hun Suh ),조광현 ( Kwang Hyun Cho ) 대한피부과학회 1995 대한피부과학회지 Vol.33 No.5

Sarcoidosis is a rare d:sease in Korea and its association with erythema nodosurn is even rarer. Recently we saw a patient of bihilar adenopathy, who had arthralgia and erythematous nodules of both lower legs. A skin biopsy obtained from the erythematous nodule of the lower leg showed septal panniculitis, consitent with erythema nodosum, and a biopsy from the mediastinal lymph node revealed a pathology consistent with sarcoidosis. This is the first case of Lofgrens syndrome confirmed by histology ir, Korea. (Kor J Dermatol 1995;33(5): 931-934)

원발성 피부 CD30 양성 대형세포 림프종

양성규 ( Seong Gyu Yang ),조광현 ( Kwang Hyun Cho ),김방순 ( Bang Soon Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Primary cutaneous CD30(Ki- 1) positive large cell lymphoma occurs generally in adults and in contrast to primary noncutaneous CD30 positive large cell lymphoma, rarely in children and adolescences. Generally it does not express epithelial membrane antigen(EMA). Recently we experienced a sixteen-year old boy who developed CD30 positive large cell lymphoma. He presented with single ulcerative nodule on his right upper vermilion border and there was no evidence of lymph nodal or other visceral involvement. In paraffin section, stainings for CD30, CD45RO, CD45 and EMA were positive but staining for CD20 was negative. His young age of onset and positive immunoreactivity to EMA of tumor cells in this case are unusual features for primary cutaneous CD30 positive large cell lymphoma. (Kor J Dermatol 1996;34(2): 331-335)

남아에 발생한 유아기 근섬유종증

양성규 ( Seong Gyu Yang ),조광현 ( Kwang Hyun Cho ),김석화 ( Suk Wha Kim ),김철우 ( Chul Woo Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

Infantile myofibromatosis is a rare specific fibrous proliferations of infancy and childhood, but its clinical and histopathological natures are well described. In Korea, only two cases have been reported. We present an additional case of infantile myofibromatosis that developed on the left proximal palm of tow-month-old male infant since birth. The tumor was brownish to red in color, 2.5x 4.0cm in size and firmly palpated. The tumor was not only localized to the skin, but also in- volved the underlying 4th and 5th metacarpal bone so that caused hypoplasia of those bones. (Kor J Dermatol 1996;34(5): 827-831)

Design of 4-Axis Scara-Type Robot for Attaching and Detaching Workpieces of Machine Tools

양성규(Seong-Gyu Yang),김갑순(Gab-Soon Kim) Korean Society for Precision Engineering 2021 한국정밀공학회지 Vol.38 No.5

This paper describes the design of a 4-axis SCARA-Type robot in the form of a scalar robot for the loading and unloading of workpieces in machine tools. The 4-axis dedicated robot is a 4-degrees-of-freedom robot consisting of a joint 1, 2, 3 motor and a 180° rotating gripper made up of a horizontal gripper and a vertical gripper. It was designed in a scalar shape that is suitable for machine tools, and the size of each link and elbow was determined through structural analysis. Through additional structural analysis, the deflection of the end center of the workpiece fixed to the horizontal gripper and the vertical gripper was designed to be within 0.1 mm, and based on the design result, a 4-axis SCARA-Type robot was manufactured, and the basic motion characteristics of the manufactured robot were tested. As a result of the characteristic test, the manufactured 4-axis SCARA-Type robot operated smoothly, so it is judged to be adequate for usage in loading and unloading the workpieces in machine tools.

Recent medical disputes alarming dermatologists

양성규 ( Seong Gyu Yang ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Intense Pulsed Light의 유래, 작용 원리와 임상 진료에서의 올바른 사용

조성문 ( Seong Moon Jo ),김방순 ( Bang Soon Kim ),김혜성 ( Hei Sung Kim ),고주연 ( Joo Yeon Ko ),노미령 ( Mi Ryung Roh ),양성규 ( Seong Gyu Yang ),유화정 ( Hwa Jung Ryu ),최재우 ( Jae Woo Choi ),황지환 ( Ji Hwan Hwang ),박현선 ( H 대한피부과학회 2013 大韓皮膚科學會誌 Vol.51 No.11

Intense pulsed light (IPL) is a high-intensity polychromatic incoherent light of a determined wavelength spectrum, fluence, and pulse duration. Clinicians can combine these variable factors of IPL for the purpose of selective destruction of target chromophores. Due to these properties, IPL has been widely used for numerous indications including hair removal and treatment of vascular or pigmented lesions. For skilled and experienced clinicians, IPL is a great treatment modality; however it can be a source of serious adverse effects when performed by untrained clinicians. Thus, to promote the proper and safe use of IPL, we summarized the origin, history, basic principles, and clinical application of IPL and discussed qualification factors needed for clinicians who use IPL. (Korean J Dermatol 2013;51(11):845∼850)

피부에 발생한 다형성 T 세포 림프종

김석우,양성규,조광현,김철우 ( Seok Woo Kim,Seong Gyu Yang,Kwang Hyun Cho,Chul Woo Kim ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.6

Cutaneous T-cell lyrr,iphomas other than mycosis fungoides and S6zary syndrome are heterogeneous : they deseve further scientific attention about their natural history and effective therapy. Pleomorphic T-cell lymphoma is a recently defined lymphoma type that can occur in the skin. We report the case of a man in whom such a tumor manifested itself with mult,iple subcuta- neous lesions. The skin b!opsy specimen showed diffuse dermal infiltration of atypical lymphocytes with highly pleomorphic nuclei. Testing for the antibody against HTLV-1 was negative and immunohistochemical staiiiing was compatible with pleomorphic T-cell lymphoma. (Korean J Dermatol 1997;35(6): 1258-1262)

멜라닌세포가 포함된 인공표피의 배양

조광현,이동윤,양성규,여운철,박경찬,윤재일 ( Kwang Hyun Cho,Dong Youn Lee,Seong Gyu Yang,Un Cheol Yeo,Kyoung Chan Park,Jai Il Youn ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.3

Background: Melanocytes grown in pure monolayer culure lack many of the cellular interactions that exist in vivo. This can be partially overcome by growing melanocytes together with other epidermal cells in skin equivalent models. Objective : The objective of the present study was to grow human melanocytes in human epidermis reconstructed on dermal substrates in vitro and to examine their response to UV radiation. Methods : The skin equivalents were prepared by seeding cultured human keratinocytes together with cultured human melanocytes(in a ratio of 5%) onto de-epidermized dermis. After 7 days of culture, they were exposed to UVB irradiation(total 150m J/cm over 5days). On day 12 of air exposure the sections of the skin equivalents were prepared for histology. The structure of the skin equivalents was studied following staining with hematoxylin and eosin. Melanocytes were characterized by DOPA staining and by immunohistochemistry. Results : Melanocytes were localized singly within the basal layer of the reconstructs. Melanin was also visible both in the melanocytes and in neighboring keratinocytes. There was an increase in melanocyte size and dendricity following UV irradiation. Melanocytes became positive to staining with HMB-45 antibody following UV irradiation. Conclusion : Our results indicate that melanocytes grown in reconstructed human epidermis are functional and capable of responding to UV irradiation. (Kor J Dermatol 1997;35(3): 526-531)

피부에 발생한 혈관 중심성 T - 세포 림프종

조광현 ( Kwang Hyun Cho ),양성규 ( Seong Gyu Yang ),김철우 ( Chul Woo Kim ),지두현 ( Doo Hyun Chi ),고재경 ( Jai Kyoung Koh ),김영근 ( Young Keun Kim ) 대한피부과학회 1996 大韓皮膚科學會誌 Vol.34 No.6

Background: Angiocentric T-cell lymphomas are rare T-cell malignancies which involve extranodal sites, such as the skin, nasal cavity, soft tissue and gastrointestinal tract. They have been reported with significant frequency in Asia. Objectives . The main objective of this study is to characterize t.he clinical, pathological, and immunohistochemical featnres of cutaneous angiocentric T-cell lymphoma. Another objective is to search for the Epstein-Barr virus (EBV) in the tissues of cutaneous angiocentiric T cell lymphoma. Methods : Clinical records, laboratory data, and histopathologic sections of 12 patients with cutaneous angiocentric T-cell lymphoma were reviewed. Paraffin tumor tissues were immunophenotyped. In situ hybridizaion studies were performed to detect the EBV genomes. Results : The ages of the 12 patients ranged from 34 to 64 years(mean 45.8 years). The cuta- neous lesions were nodules or plaqes, and were with ulcerated or had intact skin. Eight patients had evidence of extracutaneous involvement, usually involving lymph nodes, liver, and spleen. Eleven patients showed the abnormal laboratory findings including anemia, leukopenia, and elevated level of LDH. The dis ase pursued an aggressive course and was not uncommonly resistant to treatment. Hist,ologically, the lymphomatous infiltrate occurred predominantly in the subcutaneous layer with involvement f the dermis. The pattern was mainly perivascular and periadnexal. A prominent feature was invasion of small or medium vesselsby lymphoma cells. The infiltrating lymphrcytes expressed CD45RO in all cases ; variable expression of CD3 and CD56 was detected in piaffin sections. Among the 11 cases where in situ hybridization was performed, EBV genome could be detected in 9 cases. Conclusion . Angiocentvic T-cell lymphoma of the skin is an aggressive lymphoma distinct from classic cutaneous T-cell lymphoma. However, further studies are needed to regard them as a ho- mogeneous entity of T-cell lymphoma involving the skin. (Kor J Dermatol 1996;34(6): 953-960)