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생쥐에 있어서 투명대의 용해성과 분리할구의 발생 가능성에 관한 연구
양보석,임경순,이용빈 ( B . S . Yang,K . S . Im,Y . B . Lee ) 한국축산학회 1985 한국축산학회지 Vol.27 No.11
The present study was carried out to investigate the cloning of mouse embroys. Female ICR micx were superovulated by intraperitonial injection of PMSG and HCG. Two- and four-cell embryos were digested by proteolytic enzymes; pronase, trypsin, protease and pepsin, and lipase, and separated in vitro by fine glass needles with hand and isolated blastomeres were cultured in vitro for 72 hours. The results obtained were summarized as follow; 1. The proteolytic enzymes had been shown to cause dissolution of zona pellucida, but lipase didn`t. 2. In two-cell mouse embryos, the rate of dichotomy was 89.7% and 97.6 k in zona softened and removed, respectively. Among the dichotomized two-cell mouse embryos, one half and both halves undamaged blastomeres were 30.8% and 57.9% in zone softened and 12.2% and 85.4% in zona removed, respectively. 3. In four-cell mouse embryos, the rate of dichotomy was 85.3% and 96.0% in zona softened and removed. The rate of 4:0, 3:1, 2:2 and 1:3 (undamaged blastomeres vs. damaged blastomeres) were 8.8%, 15.7%, 42.2% and 18.6% in zona softened and 16.8%, 30.9%, 35.6% and 12.8% in zona removed, respectively. 4. The developmental rate of zona softened and removed single blastomeres was 68.5% and 69.0% in 2cell embryo, and 46.7% and 38.5% in 4-cell embryo, respectively. And the developmental rate of zona softened and removed single blastomeres was 10.5% and 6.9% in 2~ell embryo, and 0% and 0% in 4-cell embryo, respectively. The blastomeres isolated from 2-cell embryo developed into blastocyst, but that from 4-cell embryo developed into 8-cell stage, respectively.
梁保錫(B. S. Yang),安永供(Y. K. Ahn),崔沅鎬(W. H. Choi) 한국해양공학회 1992 韓國海洋工學會誌 Vol.6 No.2
This paper presents an approach for the derivation of frequency-dependent element matrices for vibration analysis of piping systems containing a moving medium. The dynamic stiffness matrix is deduced from transfer matrix, and, in turn, the frequency-dependent element matrices are derived. Numerical examples show that this method gives more accurate results than those obtained using the conventional static shape function based element matrices.
오성종,양보석,김희석,이근상,김강식,스피어스,아우리 ( S . J . Oh,B . S . Yang,H . S . Kim,K . S . Lee,K . S . Kim,J . Spears,H . M . Oury ) 한국축산학회 1986 한국축산학회지 Vol.28 No.7
This study was conducted to investigate the effects of breed and parity of recipients, grade of frozen-thawed embryos and degree of donor-recipients synchrony on conception rate when the frozen-thawed bovine embryos were transferred to 60 heads of Korean Native cattle (KNC), Holstein and crossbred in Livestock Experiment Station, Rural Development Administration, and nearby large farms. To synchronize the estrus, regularly cycling heifers and cows of 114 heads were injected with 50㎍ of cloprosternol twice 11 days apart. The results obtained from this study were summarized as follows; 1. Cows showed estrus with the range of 2-6 days after the first shot and 2.5-4 days after the second shot. The rate of response was higher in the second shot (82.5%) than the first shot (67.5%). 2. The rate of response to cloprostenol after the first shot was high in crossbred, Holstein and KNC in the order, although no significant differences were noted in the second shot. 3. The overall conception rate was 45.0% and that of Holstein was 52.2% (24/46) and that of KNC was 37.5% (3/8). However none of crossbred was pregnant. 4. The conception rate of heifers (46.9%) was slightly higher than that (42.9%) of cows. 5. The conception rate of cattle transferred with excellent, good and fair embryos were 68.9%, 35.9% and 40.9%, respectively. 6. The conception rate was highest when the recipients began to show estrus 12 hours later than donor did.