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해수와 피조개에서 Vibrio mimicus K-1 의 생존에 대한 보관온도의 영향
고병호(Byeong Ho Koh),이원동(Won Dong Lee),안성기(Sung Kee Ann),김지희(Ji Hoe Kim),이명숙(Myung Suk Lee) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.2
The cell density changes of Vibrio mimicus K-1 in sea water and arkshell feeding it were examined at various temperature. The strain was suspended in sterilized sea water and storaged at experimental temperature (5, 10, 15, 20, and 28℃). At intervals of up to 10 days, aliquots of each suspension were plated onto BHI agar. At 5 and 10℃, the plate counts of V. mimicus K-1 showed a rapid decline, which is known to be a result of this bacterium`s entering into the viable but non culturable state. At 20 and 28℃, however, V. mimicus K-1 are stable over the 10 days experimental periods. V. mimicus K-1 was fed to arkshell, which was subsequently stored at temperatures ranging from 5 to 20℃ for 10 days. The samples of arkshell were homogenized and plated at intervals to determine the cell density of V. mimicus K-1 and total aerobic population of bacteria present. At 5 and 10℃, the numbers of V. mimicus K-1 in sea water rapid decreased over the 10 days experimental periods. However, little change of V. mimicus K-1 densiiy was observed in shellstock arkshell at 5 and 10℃. While, V. mimicus K-1 density was decreased more rapidly to level below limit of dectection in shucked arkshell at same temperature. Incubation at the higher temperature (20℃) resulted in large increase in total aerobic bacterial number of shellstock arkshell. These results suggest that even with proper storage, indigenous levels of V. mimicus may remain sufficiently high in shellstock arkshell to produce infection in compromise hosts.
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