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Partial Cloning of ACO gene in Carnation : Dianthus caryophyllus L. cv. Yellow Liberty
Ahn, W.G.,Son, K.C.,Kim, S.Y.,Oh, S.-E. 建國大學校基礎科學硏究所 2003 理學論集 Vol.28 No.-
For the cloning of ACO(ACC-oxidase) gene, RT-PCR was carried out to amplify the nucleotides encoding conserved domains of ACO protein in carnation cv. Yellow Liberty 563 bp of RCR product was subcloned, sequenced, and named ACO-1. The amino acid sequence of ACO-1 was same as ACO proteins in other carnation cultivars. Partial cloned ACO-1 was revealed to detect effectively variation in the amount of transcript of ACO in carnation cv. Pink Donor treated with chemicals influencing the ACO activity.