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      • 초산류프롤리드 투여에 의한 흰주의 혈중 testosterone 억제와 뇌 시상하부에서 c-Fos의 발현

        서성곤,김원식,박목순,박진규,명평근 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

        Leuprolide acetate is a potent luteinizing hormone-releasing hormone analogue [leuprorelin, (des-GlylO-D-Leu6-Pro-NHEt9)-LHRH acetate]. Lorelin depot, which is composed of leuprolide acetate, was designed for one-month release injectable and biodegradable microsphere of multiple high doses. Here we compared the efficacy of microsphere lorelin depot with that of Takeda microsphere (Lucrin Depot). Lorelin (leuprolide 3.75 mg/kg of body weight) was administered s.c. to male rats, and the serum was obtained from rat tail vein. Enzyme immunoassay ( ELA ) for testosterone was carried out to assess the effect of lorelin depot. The rats were sacrificed 42 days after the lorelin β injection, and c-Fos changes of brain were observed by indirect immunofluorescence method. Results showed that the testosterone secretion was inhibited and the expression of c-Fos was also inhibited in the brain.

      • 초산류프롤리드 투여에 의한 흰쥐의 혈중 testosterone 억제와 뇌 시상하부에서 c-Fos의 발현

        서성곤,김원식,박목순,박진규,명평근 충남대학교 약학대학 의약품개발연구소 2003 藥學論文集 Vol.18 No.-

        Leuprolide acetate is a potent luteinizing hormone-releasing hormone analogue [leuprorelin, (des-Glyl0-D-Leu6-Pro-NHEt9)-LHRH acetate]. Lorelin depot, which is composed of leuprolide acetate, was designed for one-month release injectable and biodegradable microsphere of multiple high doses Here we compared the efficacy of microsphere lorelin depot with that of Takeda microsphere (Lucrin Depot). Lorelin (leuprolide 375 mg/kg of body weight) was administered sc to male rats, and the serum was obtained from rat tail vein. Enzyme immunoassay (EIA) for testosterone was carried out to assess the effect of lorelin depot. The rats were sacrificed 42 days after the lorelin β injection. and c-Fos changes of brain were observed by indirect immunofluorescence method. Results showed that the testosterone secretion was inhibited and the expression of c-Fos was also inhibited in the brain.

      • 초산류프롤리드 투여에 의한 흰 쥐 brain에의 영향과 자궁내막암 세포주에 대한 세포독성

        서성곤,김원식,명평근 충남대학교 약학대학 의약품개발연구소 2002 藥學論文集 Vol.17 No.-

        Leuprolide acetate is a potent luteinizing hormone-releasing hormone analogue [leuprorelin, (des-Glyl0-D-Leu6-Pro-NHEt9)-LHRH acetate]. Lorelin depot, which was composed of leuprolide acetate, was designed for one-month release injectable and biodegradable microsphere of multiple high doses. Here we examined the effect of microsphere lorelin depot, in comparison with Takeda microsphere (Lucrin Depot). Lorelin (leuprolide 3.75 ㎎/㎏ of body weight) was administered subcutaneous to male rats. The rats were sacrificed after 42 days and morphological changes of brain were observed by light microscopy. Leuprolide acetate has been shown to have an inhibitory effect on the growth of several hormone-dependent human tumors, So, leuprolide actate was examined for the cell cytotoxicity of the human endometrial cancer cell line, SNU-685, which was characterized in primary tumors of Korean patient and other cancer cell lines, MCF-7, T-24 and FB-l3P Cells were cultured with indicated concentrations of leuprolide acetate for 48 hr. Antiproliferative effect on SNU-685 cell which were determined by colorimetric method, MTT and MTS/PMS, was shown 20%, but those effects on MCF-7, T24, FB-23P cells were little shown.

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