개조개(Veneridae Saxidomus purpuratus Sowerby)의 소화효소에 대하여 (제 2 보) Proteinase의 효소적성질

서석수(Suk Soo Suh),양한석(Han Suk Yang),홍성철(Sung Chel Hong) 대한약학회 1959 약학회지 Vol.4 No.1

The enzymatic activity of proteinase which was isolated from a shell fish, Veneridae Soxidomus purpuratus Sowerby (Korean name "Gai-io-gai") was studied, and the obtained results were as follows;(l) The optimum pH of the enzyme was around 7. 5 (2) The prohibiting activity of metallic ions for the enzymatic activity was the order of 1/1000 M-Ag->1/1000M -Zn++>1/1000M-Cd++>1/1000M-Pb++. (3) Of 3 specimens of the enzyme from heptapancreas, gastro-intestine and crystalline style the highest activity was shown by one from crystalline style.

우무가사리(Gelidium amansii)의 구충성분에 대하여(제 1 보)

서석수(Suk Soo Suh),홍성철(Sung Chel Hong) 대한약학회 1962 약학회지 Vol.6 No.2

Clinical tests revealed that the extracts of Gelidium amansii (Gelidiaceae) had a anthelmintic action and further examinations were made on the anthelmintic components of this seaweed. This anthelmintic principle is adsorbed on alumina and eluted from it by alkali solution. The active principle is adsorbed on activated carbon from aqueous extract and eluted from it by methanol and it is not adsorbed on Amberite IR-120(H-form). This anthelmintic effective fraction was prepared by the use of this properties. Action of the active principle of Gelidium amansii was examined pharmacologically. The active principle of Gelidium amansii was found to decrease the tensity, tonus and mobility of Eisenia foetida(Savigny) nerve muscles. The active principle of this effective fraction was submitted to paper chromatography and spots to ninhydrin were detected at, Rf; 0.30~0.31(yellow), 0.26(violet), 0.2(violet), 0.14~0.13(violet), 0.9(orange) and 0.04(violet).

개조개(Veneridal Soxidmus Purpuratus Sowerby)의 소화효소에 대하여 (제1보) Amylase의 효소적성질

서석수(Suk Soo Suh),홍승철(Sung Chel Hong),양한석(Han Suk Yang) 대한약학회 1959 약학회지 Vol.4 No.1

The enzymatic activity of amylase which was isolated from a shell fish Veneridae Soxidmnus purpuratus Sowerby(Korean name"Gai-jo-gai") was studied and the obtained results were as follows: (1) The optimum pH of the enzyme was Ca. 6.2~6.4. (2) Prohibiting activity of metallic ions for the enzymatic activity was the order of 1/1000M-Mg++>1/1000M-Sr++> 1/000M-Na+, and Ca++ ion''s prohibiting action was hardly showed. (3) Of 3specimens of amylase from Heptapancreas, Gastro-intestine and crystalline style, the highest activity was shown by amylase from crystalline style, and the other two showed almost same degree of activity. (4) Heptapancreas Amylase from the shell fish showed remarkably higher enzymatic activity than the pancreas amylase from a pig.

우릉쉥이(Cynthia roretzi v. Drasche)의 소화효소에 대하여 (제1보) Amylase의 효소적 성질

서석수(Suk Soo Suh),양한석(Han Suk Yang) 대한약학회 1960 약학회지 Vol.5 No.1

Some enzymatic properties of Cynthia roretzi v. (Drasche Korean : U-Rung-Shei) was studied by author and obtained the following results; Amylase 1. The optimum pH of the digestive gland amylase was 6.8-7.0 2. Activity of metallic ion on the amylase showed the following order; 10-3M Mn++>10-3Mco++> 10-4M Mg++>10-4M Ca++>10-3M Sr++. Inhibition of metallic ion on the amylase showed following order; 10-2M Cd++>10-2M Zn++>10-2M Pb++ 3. The digestive gland enzyme inactivated at 70oC. 4. When the enzyme concentration increase 2 times, the enzymatic activity also increase, but not proportionally. 5. The digestine gland amylase showed remarkably higher enzymatic activity than the intestinal amylase. 6. The digestive gland amylase from the ascidian showed remarkably higher enzymatic activity than the heptancreatic amylase from shell fish (Turbo (Batillus) Cornutus Solander).

우릉쉥이(Cynthia roretzi v. Drasche)의 소화효소에 대하여 (제2보) Proteinase의 효소적 성질

서석수(Suk Soo Suh),양한석(Han Suk Yang) 대한약학회 1960 약학회지 Vol.5 No.1

Some enzymatic properties of Cynthia roretzi V. Drasche (Korean: U-Rung-Shei) was studied by author. and obtained the following results; 1.The optimum pH of the digestive gland proteinase was 7.4~7.6 2.Activity of metallic ion on the proteinase showed the following order; 10-3 M. Mn++>10-3M. Co++>10-4 M. Mg++>10-2 M. Sr++ Inhibrtion of metallic ion on the Proteinase showed following order; 10-3M. Ag+>10-3M.cd++>10-3 M. Pb++>10-3 M. Zn++ 3.The digestive gland enzyme inactivated at 70oC, but no influence at 50oC. 4.When the enzyme Concentration increase 2times, and 3times, the enzymatic activity also increase, but not proportionally 5.The digestive gland Proteinase showed remarkably higher enzymatic activity than the intestinal Proteinase. 6.The digestive gland amylase brom the ascidion showed remarkably higher enzymatic activity than the heptaponcreatic amylase from shell fish (Turbo (Batillus) Cornutus Solander.)

TMV mRNA 의 in Vitro Translation 을 선택적으로 억제하는 Anti - Sense DNA 및 RNA Oligonucleotide

이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2

Three different antisense oligonucleotides and their RNA analogues, each complementary to 5`-end and 3`-end sequences of Tobacco Mosaic Virus (TMV) mRNA, inhibit in vitro translation of the genomic RNA in a rabbit reticulocyte lysate. Inhibition depends upon the concentration, binding sites of antisense oligonucleotides and the secondary structure of TMV mRNA. DNA oligonucleotides and their RNA analogues complementary to 5`-end regions of TMV RNA have the same effects on inhibition of translation. However, oligonucleotides complementary to 3`-end TMV RNA regions, which are supposed to form pseudoknotted RNA structure, have different effects on inhibition of translation. When DNA antisense oligonucleotides are used for inhibition of translation in wheat germ extract containing RNase H, then they have higher efficiency for inhibition of translation compared to rabbit reticulocyte lysate. We conclude that the design of antisense oligonucleotide depends on the secondary structure of target genes and their intrinsic components.

Telomere 염기배열을 포함하는 DNA , RNA 및 RNA - DNA Hybrid Oligonucleotide 의 구조에 관한 연구

이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5

Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1∼5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence d(T₂G₄)₄. T2, T3, T4 and T5 were d(T₂AG₃)₄, h(U₂dG₄)₄, h(T₂rG₄)₄ and r(U₂G₄)₄, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5`-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer h(U₂dG₄)₄, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer h(T₂rG₄)₄, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer r(U₂G₄)₄ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.

Channel Catifish Liver 에 중금속 처리로 유도되는 Metallothiolein mRNA 및 Metallothionetionein - Iike 단백질의 합성에 관한 연구

이소영,이광문,서석수,송영환,이복률 ( So Young Lee,Kwang Moon Lee,Suk Soo Suh,Young Hwan Song,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1

When channel catfish was injected with Cd and Zn chloride, the liver of the Cd-treated channel catfish was transiently activated to synthesize metallothionein(MT) mRNA and MT-like proteins. The poly(A)^+ mRNA of about 400 bases long was detected in the Cd-treated channel catfish liver with synthetic DNA probes (27 mer), which were conserved sequences in rainbow trout MT cDNA and human MT cDNA, by northern blot hybridization experiment. Amount of the mRNA from Cd treated channel catfish liver was enough to detect translation products in vitro, using the rabbit reticulocytelysate cell-free translation system. These translation products were MT-like proteins, which were about 6.5 kD in size on SDS-PAGE.

Structure of Telomeric DNA, RNA and DNA-RNA Hybrid Oligonucleotides by Nondenaturing Polyacrylamide Gel Electrophoresis

이광문,이소영,서석수,이복률,Lee, Kwang-Moon,Lee, So-Young,Suh, Suk-Soo,Lee, Bok-Luel Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.5

Tetrahymena human의 Telomere 염기배열을 가지는 DNA, RNA 및 DNA-RNA hybrid oligonucleotide를 다섯 종류 화학 합성하여 전기영동의 gel mobility shift 실험, Tm 측정 및 DMS methylation 반응으로 그들의 구조를 구명하였다. 합성한 oligonucleotide(T1~5) 의 염기배열은 다음과 같다. T1 : d$d(T_2G_4)_4$, T2 : $d(T_2AG_3)_4$, T3: $h(U_2dG_4)_4$, T4 : $h(T_2rG_4)_4$, T5: $r(U_2G_4)_4$. 이들에 대한 nondenaturing PAGE에서 gel mobility 실험결과 T1과 T2는 size marker DNA 24mer보다 훨씬 빠른 gel mobility를 나타내었고, Tm 측정 및 gel mobility 실험결과 T1은 T2보다 안정한 구조를 취하는 것을 확인하였다. 또한, DMS methylation 실험결과 5'말단의 dG가 methylation반응에 sensitive한 것을 확인하였다. 이상의 결과로서 T1 및 T2 oligoneotide는 용액 중에서 Hoogsteen 염기대를 형성하는 Telmere 구조를 취하는 것을 확인하였고. T1 oligoneotide의 염기배열에서 dT를 rU로 치환한 T3는 T1과 동일구조를 취하는 것이 확인되었으나, dG가 rG로 치환된 T4는 T1과는 다른 gel mobility를 나타내었지만, T5와는 동일한 gel mobility를 나타내었다. 이상의 결과로서 분자내 수소결합으로 Hairpin 구조를 취하는 Telomere 구조형성에 가장 중요한 염기는 deoxyguanosine임을 알았다. Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1~5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence $d(T_2G_4)_4$. T2, T3, T4 and T5 were $d(T_2AG_3)_4$, $h(U_2dG_4)_4$, $h(T_2rG_4)_4$ and $r(U_2G_4)_4$, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5'-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer $h(U_2dG_4)_4$, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer $h(U_2dG_4)_4$, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer$r(U_2G_4)_4$ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.

The Pharmaco - chemical Study on the Plant of Ixeris spp. - 1. Anti - hypercholesterolemic Effect of Ixeris sonchifolia

양한석(Han-Suk Young),서석수(Suk-Soo Suh),이경희(Kyung-Hee Lee),이지현(Ji-Hyon Lee),최재수(Jae-Sue Choi) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.3

고들빼기의 부위별 메탄올 엑스가 고콜레스테롤 생쥐의 혈청 콜레스테롤농도에 미치는 영향을 살펴보았다. 고콜레스테롤 생쥐는 식이에 1% 콜레스테롤과 0.5% 답즙산을 첨가하므로서 유도하였으며, 잎의 메탄올 엑스를 투여한 군은 대조군에 비해 혈청 콜레스테롤 농도를 현저히 감소시켰으나 뿌리의 경우에는 효과가 나타나지 않았다 . 또한, 잎의 메탄올 엑스는 고콜레스테롤 흰쥐에 대해서도 100㎎/㎏ 투여 용량에서 혈청 콜레스테롤 농도를 현저히 감소 시켰으며 동맥경화성 지표를 개선시켰다. 반면에, 정상흰쥐에 대해서는 효과가 나타나지 않았다. 이상과 같은 결과로 잎의 메탄올 엑스는 과잉의 콜레스테롤을 섭취하였을때 체내대사 이용도를 증가시키므로서 혈청 콜레스테롤 농도를 감소시킨다고 생각된다. The methanol extracts from different parts of Ixeris sonchifolia (Compositae) were evaluated for their total cholesterol lowering effect in mice. Mice were rendered hypercholesterolemic with 1% cholesterol and 0.5% cholic acid. Significant lowering in serum cholesterol was observed in mice with the methanol extract from leaves (MeOH-LF), whereas the methanol extract from roots (MeOH-RT) was devoid of this effect. In rats with cholesterol-induced hyperlipidemia MeOH-LF in a dose of 100㎎/㎏ body weight caused significant decrease of total cholesterol, and the atherogenic index was also improved. On the other hand, total cholesterol in rats fed a stock diet was not affected by administration of the MeOH-LF. Thus, it is suggested that this MeOH-LF probably may increase the metabolic utilization only when fed with excess cholesterol.