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RISS 인기검색어
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- 저자 : 이복률 ( So Young Lee (검색결과 6 건)
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이광문,이소영,서석수,이복률,Lee, Kwang-Moon,Lee, So-Young,Suh, Suk-Soo,Lee, Bok-Luel Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.5
Tetrahymena human의 Telomere 염기배열을 가지는 DNA, RNA 및 DNA-RNA hybrid oligonucleotide를 다섯 종류 화학 합성하여 전기영동의 gel mobility shift 실험, Tm 측정 및 DMS methylation 반응으로 그들의 구조를 구명하였다. 합성한 oligonucleotide(T1~5) 의 염기배열은 다음과 같다. T1 : d$d(T_2G_4)_4$, T2 : $d(T_2AG_3)_4$, T3: $h(U_2dG_4)_4$, T4 : $h(T_2rG_4)_4$, T5: $r(U_2G_4)_4$. 이들에 대한 nondenaturing PAGE에서 gel mobility 실험결과 T1과 T2는 size marker DNA 24mer보다 훨씬 빠른 gel mobility를 나타내었고, Tm 측정 및 gel mobility 실험결과 T1은 T2보다 안정한 구조를 취하는 것을 확인하였다. 또한, DMS methylation 실험결과 5'말단의 dG가 methylation반응에 sensitive한 것을 확인하였다. 이상의 결과로서 T1 및 T2 oligoneotide는 용액 중에서 Hoogsteen 염기대를 형성하는 Telmere 구조를 취하는 것을 확인하였고. T1 oligoneotide의 염기배열에서 dT를 rU로 치환한 T3는 T1과 동일구조를 취하는 것이 확인되었으나, dG가 rG로 치환된 T4는 T1과는 다른 gel mobility를 나타내었지만, T5와는 동일한 gel mobility를 나타내었다. 이상의 결과로서 분자내 수소결합으로 Hairpin 구조를 취하는 Telomere 구조형성에 가장 중요한 염기는 deoxyguanosine임을 알았다. Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1~5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence $d(T_2G_4)_4$. T2, T3, T4 and T5 were $d(T_2AG_3)_4$, $h(U_2dG_4)_4$, $h(T_2rG_4)_4$ and $r(U_2G_4)_4$, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5'-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer $h(U_2dG_4)_4$, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer $h(U_2dG_4)_4$, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer$r(U_2G_4)_4$ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.
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이소영,이광문,서석수,송영환,이복률 ( So Young Lee,Kwang Moon Lee,Suk Soo Suh,Young Hwan Song,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1
When channel catfish was injected with Cd and Zn chloride, the liver of the Cd-treated channel catfish was transiently activated to synthesize metallothionein(MT) mRNA and MT-like proteins. The poly(A)^+ mRNA of about 400 bases long was detected in the Cd-treated channel catfish liver with synthetic DNA probes (27 mer), which were conserved sequences in rainbow trout MT cDNA and human MT cDNA, by northern blot hybridization experiment. Amount of the mRNA from Cd treated channel catfish liver was enough to detect translation products in vitro, using the rabbit reticulocytelysate cell-free translation system. These translation products were MT-like proteins, which were about 6.5 kD in size on SDS-PAGE.
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TMV mRNA 의 in Vitro Translation 을 선택적으로 억제하는 Anti - Sense DNA 및 RNA Oligonucleotide
이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
Three different antisense oligonucleotides and their RNA analogues, each complementary to 5`-end and 3`-end sequences of Tobacco Mosaic Virus (TMV) mRNA, inhibit in vitro translation of the genomic RNA in a rabbit reticulocyte lysate. Inhibition depends upon the concentration, binding sites of antisense oligonucleotides and the secondary structure of TMV mRNA. DNA oligonucleotides and their RNA analogues complementary to 5`-end regions of TMV RNA have the same effects on inhibition of translation. However, oligonucleotides complementary to 3`-end TMV RNA regions, which are supposed to form pseudoknotted RNA structure, have different effects on inhibition of translation. When DNA antisense oligonucleotides are used for inhibition of translation in wheat germ extract containing RNase H, then they have higher efficiency for inhibition of translation compared to rabbit reticulocyte lysate. We conclude that the design of antisense oligonucleotide depends on the secondary structure of target genes and their intrinsic components.
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Telomere 염기배열을 포함하는 DNA , RNA 및 RNA - DNA Hybrid Oligonucleotide 의 구조에 관한 연구
이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5
Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1∼5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence d(T₂G₄)₄. T2, T3, T4 and T5 were d(T₂AG₃)₄, h(U₂dG₄)₄, h(T₂rG₄)₄ and r(U₂G₄)₄, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5`-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer h(U₂dG₄)₄, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer h(T₂rG₄)₄, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer r(U₂G₄)₄ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.
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Lee, So Young,Moon, Hyun Joo,Kurata, Shoichiro,Kurama, Takeshi,Natori, Shunji,Lee, Bok Leul 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-
Injection of Escherichia coli into larvae of the coleopteran Holotrichia dimphalia results in the appearance of antibacterial activity in the hemolymph. An antibacterial protein, named holotricin 2, was purified from larvae of this inset and characterized. A cDNA clone for holotricin 2 was isolated and its complete sequence was determined. This protein was found to inhibit the growth of Gram-negative bacteria and to consist of 72-amino acid residues with no cysteine residues. Its amino acid sequence is similar to that of coleoptericine, an antibacterial protein isolated from larvae of the coleopteran Zophobas atratus.
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Moon, Hyun Joo,Lee, So Young,Kurata, Shoichiro,Natori, Shunji,Lee, Bok Luel 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-
Antibacterial activity was induced in the hemolymph of larvae of the coleopteran Tenebrio molitor by injection of Escherichia coli. An antibacterial oritein, named tenecin 1, was purified to homogeneity from the larval hemolymph and characterized . A cDNAC clone for tenecin 1 was isolated and its complete sequence was determined. This protein was fiund to inhibit the growth of Gram-positive bacteria and to consist of 43-amino anid residues including six cysteine containing peptides obtained by digesting tenecin 1 with endopoptidase Lys-C, trypsin and thermolysin. The amino acid sequence and its disulfide bonds were similar to those of sapecin and sapecin C, antibacterial proteins of Sarcophaga peregrina.
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