바이오융합 및 의료기기 산업

박수아,이준희,김완두,Park, Su A,Lee, Jun Hee,Kim, Wan Doo 대한기계학회 2017 대한기계학회 논문집. Transactions of the KSME. C, 산업기술과 혁신 Vol.5 No.1

바이오융합 및 의료기기 산업은 의학과 전기, 전자, 기계, 재료 등 공학이 융합되는 다학제간 응용기술 산업분야이다. 바이오 융합 및 의료기기를 이용해 인간의 삶의 질 향상을 목표로 하고 있으며 제품에 대한 인지도와 브랜드 파워가 매우 중요한 산업이다. 그러나, 자본/기술 의존형 산업으로 제품의 개발부터 생산까지 소요되어지는 기간이 길고 개별 제품의 시장 규모가 작고 수명주기가 짧다고 할 수 있다. 따라서 연구개발에 대한 지속적인 투자가 요구되어지는 산업으로 국가적인 차원에서 바이오벤처 기업들을 위해 기술적 지원, 제도적 뒷받침, 인력 양성 등의 산업생태계 전반을 활성화하고자 하는 노력이 필요하다. Bio-fusion and medical device industry is the multidisciplinary engineering application technology industries, which are fused, such as electricity, electronics, machinery, materials. It aims to improve the quality of human life by using bio-fusion and medical devices, and is an important industry recognition and brand power for the product. However, there is a period that is required from the development of products with technology-dependent industries. Therefore, it is necessary to have continuous effort for industrial investment in research and development at the national level including technical support, institutional support, and human resources.

생체조직공학을 위한 3차원 스케폴드의 제작 기술 개발 동향

박수아,민태진,김근형,Park, Su-A,Min, Tae-Jin,Kim, Geun-Hyeong 재료연구소 2006 機械와 材料 Vol.18 No.3

조형가공기술을 이용한 인공지지체의 수산화나트륨 개질 효과

박수아(Su A Park),이정복(Jung Bok Lee),김양은(Yang Eun Kim),김지은(Ji Ein Kim),권일근(Il Keun Kwon),이준희(Jun Hee Lee),김완두(Wan Doo Kim),김형근(Hyung Keun Kim),김미은(Mi Ein Kim),이준식(Jun Sik Lee) 한국고분자학회 2014 폴리머 Vol.38 No.6

조직공학에서이 인공지지체는 세포으 부착과 증식 및 분화가 잘 되어야 하고, 우수한 생체친화성 및 생분해성을 지녀야 한다. 다양한 인공지지체 제작 방법이 시도되어지고 있으며, 최근들어 3D 프린팅 기술을 이용한 방식이 활발하게 연구되어지고 있다. 폴리카프로락톤(polycaprolactone, PCL)은 낮은 녹는점을 가지고 있어 3D 프린팅하기에 우수한 생체적합 고분자 합성재료이다. 본 연구에서는 3D 프린팅 기술을 이용하여 3차원 PCL 인공지지체를제작하였고, 지지체의 표면개질을 위해 수산화나트륨(NaOH)을 이용하였다. 표면개질된 인공지지체의 표면특성을SEM으로 확인한 결과, 수산화나트륨을 처리한 PCL 인공지지체가 처리하지 않은 PCL 인공지지체에 비해 거칠기가증가함을 보였으며, 접촉각 측정을 통해 친수성이 증가함을 확인하였다. In vitro 실험결과, 수산화나트륨을 처리한PCL 인공지지체가 처리하지 않은 PCL 인공지지체에 비해 세포의 증식과 분화가 증가함을 보였고, 세포의 부착 모습은 균일하고 밀집된 형태로 부착됨을 확인하였다. 따라서 조형가공기술을 이용하여 수산화나트륨을 처리한 표면개질된 PCL 인공지지체를 제작하고 분석함으로써, 세포적합성을 통해 체내 인공지지체 개발 적용 가능성을 제시하였다. Scaffolds of tissue engineering should be biocompatible and biodegradable for cell attachment, proliferationand differentiation. In the various scaffold fabrication, 3D printing technique can make the three dimensional scaffoldwith interconnected pores for cell ingrowth. Polycaprolactone (PCL) is biodegradable polyester with a low melting temperatureand has been approved by the Food and Drug Administration (FDA). In this study, PCL scaffold was fabricatedby 3D bioprinting system and surface modification of PCL scaffold was controlled by NaOH treatment. Morphologicalchange and wetability of NaOH-treated scaffold were observed by SEM and contact angle measurement system. Theremnant of PCL treated with NaOH was measured by ATR-FTIR. In vitro study of scaffolds was evaluated with WST-1 and ALP activity assay. NaOH treatment of PCL scaffolds increased surface roughness, hydrophilicity, cell proliferationand osteogenic differentiation. These results indicate that NaOH-treated PCL scaffold made by 3D bioprinting has tissueengineered potential for the development of biocompatible material.

조직공학용 스캐폴드 개발을 위한 조형가공기술의 적용

박수아 ( Su A Park ) 조선대학교 공학기술연구원 2010 공학기술논문지 Vol.3 No.1

Tissue engineering has researched as a field to replace tissues and organ lost by disease or trauma. Recently, tissue engineered scaffold fabrication for tissue substitutes was developed by rapid prototyping (RP) system using solid freeform fabrication (SFF) technology. The scaffolds fabricated by RP system have interconnetecd pore, variable mechanical property, controllable pore size and porosity. The pore structure provides space for cell migration and adhesion. In this paper, we discuss the various SFF technique and introduce the three-dimensional (3D) scaffold using RP system.

조직공학을 위한 생체모사용 스캐폴드 개발

박수아 ( Su A Park ),이준희 ( Jun Hee Lee ),김완두 ( Wan Doo Kim ) 한국고무학회 2009 엘라스토머 및 콤포지트 Vol.44 No.2

조직공학은 기능을 상실한 인체를 대체하거나 복원하기 위해 인공대체품을 개발하기 위한 중요한 학문이다. 특히, 세포가 자랄 수 있는 지지체 역할을 하는 스캐폴드는 조직공학 연구를 위한 중요한 부분을 차지하고 있다. 그래서, 3차원 조직공학용 스캐폴드 개발을 위한 다양한 제조 방법을 소개하고자 하였다. 스캐폴드의 일반적인 제조방법으로는 염침출법(solvent-casting particulate-leaching), 염 발포법 (gas foaming/salt leaching), fiber meshes/fiber bonding 법, 상분리법 (phase separation), melt moulding 법, 동결 건조법 (freeze drying)이 있으며, 넓은 표면적을 가진 스캐폴드 개발방법으로 전기방사법이 알려져 있다. 또한, 최근에는 스캐폴드 내부의 균일한 세포의 침투를 유도하기 위해 적당한 공극크기를 조절하고 우수한 공극률을 가진 스캐폴드를 개발하고자 stereo-lithography (SLA), selective laser sintering (SLS), fused deposition modeling (FDM), 및 3D printing (3DP)와 같은 다양한 solid freeform fabrication (SFF) 기술이 개발 되어지고 있다. Tissue engineering is a research field for artificial substitutes to improve or replace biological functions. Scaffolds play a important role in tissue engineering. Scaffold porosity and pore size provide adequate space, nutrient transportation and cell penetration throughout the scaffold structure. Scaffold structure is directly related to fabrication methods. This review will introduce the current technique of 3D scaffold fabrication for tissue engineering. The conventional technique for scaffold fabrication includes salt leaching, gas foaming, fiber bonding, phase seperation, melt moulding, and freeze drying. These conventional scaffold fabrication has the limitations of cell penetration and interconnectivity. In this paper, we will present the solid freeform fabrication (SFF) such as stereolithography (SLA), selective laser sintering (SLS), and fused deposition modeling (FDM), and 3D printing (3DP).

Development and application of 3D printing in biomedical field

Su A Park(박수아) 대한기계학회 2017 대한기계학회 춘추학술대회 Vol.2017 No.11

기계적 인장 자극으로 인한 마이크로패턴화된 실리콘 표면에서 세포 거동 연구

박수아(Su A Park),김인애(In Ae Kim),이용재(Yong Jae Lee),신지원(Ji Won Shin),김완두(Wan Doo Kim),신정욱(Jung-Woog Shin) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6

Ligament can be easily damaged through sports and daily activities. In this study, human ligament fibroblasts were cultivated on the micropatterned silicone surfaces and subjected to mechanical stretch to simulate the in vivo biomechanical environment of ligament healing. Cell experiments indicated that the cell arrangement was changed by micropatterned silicone surface and mechanical stretch. This study may have potentials for tissue engineering and other biomedical applications of biomaterials.

간헐적 정수압의 다양한 패턴에 따른 세포 부착력과 F-actin 및 vinculin 의 분포 변화 연구

김영직(Young Jick Kim),박수아(Su-A Park),신호준(Ho Joon Shin),김인애(In Ae Kim),이용재(Yong Jae Lee),허수진(Su-Jin Heo),황영미(Young-Mi Hwang),신정욱(Jung-Woog Shin) 한국정밀공학회 2004 한국정밀공학회 학술발표대회 논문집 Vol.2004 No.10월

Cell adhesion to any material surface is considered to be fundamental and important phenomenon in the fields of tissue engineering. Cell adhesion molecules, mechanism, and attachment force have been studied and described a lot. However, the effects of mechanical stimuli on the adhesive forces still have been left much to be investigated. In this study, to investigate the changes in cell adhesive force due to resting time period during the intermittent hydrostatic pressurizing (IHP), cells were cultured under the IHP with various resting times. Then the cell adhesive forces were measured quantitatively utilizing a cell detachment test system and immunofluorescent staining was performed using fluorescent microscopy. In the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). The results indicated that IHP can contribute in improving cell adhesive force and some of time intervals were required for the expression of cell response.

목형 잎 추출물의 항산화 활성과 멜라닌 생합성에 대한 저해활성

김아름 ( A Reum Kim ),박수아 ( Su Ah Park ),하지훈 ( Ji Hoon Ha ),박수남 ( Soo Nam Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2013 한국미생물·생명공학회지 Vol.41 No.1

본 연구에서는 목형 잎 추출물의 항산화 활성, 성분분석 그리고 mushroom tyrosinase 저해활성 측정, α-MSH로 유도된 세포 내 멜라닌 생합성에 대한 저해활성에 관한 연구를 수행하였다. 목형 잎 추출물의 자유라디칼 (1,1-diphenyl- 2-picrylhydrazyl, DPPH) 소거활성 (FSC50)은 에틸아세테이트 분획에서 14.51 μg/ml, 아글리콘 분획에서 13.96 μg/ml로 측정되었다. Luminol-의존성 화학발광법을 이용한 Fe3+- EDTA/H2O2 계에서 생성된 활성산소종 (reactive oxygen species, ROS)에 대한 목형 잎 추출물의 총항산화능은 아글리콘 분획에서 0.22 μg/ml로, 아글리콘 분획에서 가장 큰 활성을 나타내었다. 목형 잎 추출물에 대하여 rose-bengal로 증감된 1O2에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적(1~50 μg/ml)으로 증가하였으며, 특히 아글리콘 분획에서 지용성 항산화제인 (+)-α-tocopherol 보다 우수한 세포보호 활성이 있는 것으로 나타났다. Mushroom tyrosinase의 활성 저해 효과(IC50)를 측정한 결과 에틸아세테이트 분획(IC50 = 48.58 μg/ml)에서 우수한 효과를 나타났고, α-MSH로 유도된 세포 내 멜라닌 합성 저해 활성을 측정한 결과 에틸아세테이트 분획물의 50 μg/ml 농도에서 41.80% 저해활성을 나타내었다. TLC, HPLC 및 LC/ESIMS를 이용한 성분분석 결과 에틸아세테이트 분획 중에 luteolin, isoorientin이 존재함을 확인하였다. 이상의 결과들로부터 목형 잎 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 mushroom tyrosinase 저해효과와 α- MSH로 유도된 멜라닌 생합성 저해 효과로부터 에틸아세테이트 분획 중 luteolin 및 isoorientin을 새로운 미백 화장품의 원료로써 이용가능성을 알 수 있었다. The aim of this study was to evaluate various aspects of Vitex negundo L. leaf extract, such as the antioxidative activity, tyrosinase inhibitory effects, and inhibitory activities on α-MSH induced melanogenesis, and active component analysis. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC50) of the ethyl acetate fraction and aglycone fraction of V. negundo L. leaf extract were 14.51 μg/ml and 13.96 μg/ml, respectively. A luminol-dependent chemiluminescence assay revealed that the reactive oxygen species (ROS) scavenging activity (OSC50) of the aglycone fraction of V. negundo L. leaf extract on ROS generated in an Fe3+-EDTA/H2O2 system was the most prominent at 0.22 μg/ml. The protective effects of the extracts fractions of V. negundo L. leaf against the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1~50 μg/ml). In particular, there were greater protective effects of the aglycone fraction on the cellular membrane than that of the fat-soluble antioxidant (+)-α-tocopherol. The inhibitory effects (IC50) on mushroom tyrosinase were the highest for the ethyl acetate fraction (IC50 = 48.58 μg/ml). The inhibitory effect on α-MSH induced melanogenesis in B16 melanoma cells was 41.80% at 50 μg/ml of ethyl acetate fraction. Active component analyses by TLC, HPLC and LC/ESI MS revealed luteolin and isoorientin. These results indicate that V. negundo L. leaf extract can be used as an antioxidant for ROS scavenging. Particularly, the luteolin and isoorientin of the ethyl acetate fraction may be applicable to new whitening cosmetics because of its inhibitory effect on mushroom tyrosinase and α-MSH induced melanogenesis in B16 melanoma cells.

형상이 제어된 골 조직 재생용 3차원 지지체를 적용한 새로운 형태의 Perfusion Culture

이시우 ( Shi Woo Lee ),허수진 ( Su Jin Heo ),장지연 ( Ji Yeon Jang ),정재영 ( Jae Young Jeong ),김수향 ( Su Hyang Kim ),박수아 ( Su A Park ),전은수 ( Eun Su Jeon ),신정욱 ( Jung Woog Shin ) 한국조직공학·재생의학회 2010 조직공학과 재생의학 Vol.7 No.1

The objective of this study was to investigate the efficacy of a newly designed perfusion bioreactor which provides flow-induced mechanical stimulation on the cells residing in the intra-morphology controllable scaffolds. For this, we fabricated scaffolds composed of poly e-caprolactone (PCL) and micro-sized hydroxyapatite (HA) particles using rapid-prototyping process. Also a new bioreactor system for perfusion culture was designed and developed. For the analyses of cellular responses of bone-tissue related cells cultured in the perfusion bioreactor system, various biological assays were performed such as MTT test, DNA content measurement, FE-SEM and live/dead staining. The cells used in this study were MG-63 (human osteoblast-like cell line) and mesenchymal stem cells from New Zealand white rabbits. Our results showed that the cells cultured by the perfusion bioreactor resulted in higher proliferation rate and mineralization of extracellular matrices than those cultured in static culture. From this study, we could confirm the potentials of 1) a newly developed perfusion bioreactor, 2) intra-morphology controllable scaffolds composed of PCL and HA particle and 3) the combination of the suggested scaffolds and perfusion culturing system in relation to bone tissue engineering.