특성화고등학교 남학생의 금연의도 구조모형

도은수,최은숙 한국간호과학회 2017 Journal of Korean Academy of Nursing Vol.47 No.2

Purpose: This study was done to develop and test a structural model on smoking cessation intention in technical high school men. The conceptualmodel was based on the theory of reasoned action and health promotion model. Methods: From May 29 to April 13, 2015, 413 technicalhigh school students who smoked completed a structured questionnaire. Data were analyzed to calculate the direct and indirect effects of factorsaffecting smoking cessation intention. The SPSS WIN 20.0 and AMOS 21.0 programs were used. Results: The hypothetical model was a goodfit for the data. The model fit indices were χ2/df=2.36, GFI=.95, AGFI=.92, NFI=0.97, and RMSEA=.05. Self-esteem had direct and indirect effectson smoking cessation intention. Attitude, subjective norm, and self-efficacy had direct effects on smoking cessation intention. Smoking knowledgeand environmental factor had indirect effects on smoking cessation intention. This model explained 87.0% of the variance in smoking cessationintention. Conclusion: These results indicate that technical high school students’ intention to stop smoking can be improved through anincrease in self-esteem, negative environmental factors, attitude toward smoking cessation, subjective norm about smoking cessation, and selfefficacyfor smoking cessation.

키네시오 테이핑요법이 월경전후기 불편감에 미치는 효과

도은수,박경민,이성희 지역사회간호학회 2003 지역사회간호학회지 Vol.14 No.3

Purpose: This study aims to examine whether the kinesio tape method can relax perimenstrual symptoms and decrease the menstrual pain of those who complain of perimenstrual discomforts. Method: This study has been designed to use the nonequivalent control group in the pre and post-test. The data have been collected from unmarried females complaining of perimenstrual discomfort and living in D city from the 2nd of February through the 10th of April, 2002. An experimental group of 32 people and a control group of 35 people were chosen, and they were first investigated for symptoms before and after menstruation when the menstrual pain was the severest and for the VAS measurement of menstrual pain. For the experimental group, the kinesio tape method was conducted two times a week for three weeks (six times in all), and the control group remained intact. The investigations after the experimental treatment were carried out in the same way as before. The measuring instruments were the perimenstrual measuring instruments of symptoms of 42 items and the menstrual pain was measured by VAS Result: The primary hypothesis: The experimental group who used the kinesio tape method would experience less perimenstrual discomfort than the control group who did not use it. The first sub-hypothesis: The first sub-hypothesis that suggests 'The experimental group who used the kinesio tape method would experience more relaxation of perimenstrual symptom than the control group who did not useit' was supported. The second sub-hypothesis: The second sub-hypothesis that suggests that 'The experimental group who used the kinesio tape method would get lower scores of menstrual pain of VAS than the control group who did not use it' was supported. Conclusion: These results of the study show that the kinesio tape method is effective in relaxing perimenstrual symptoms and lessening menstrual pain.

인삼포장에서 뿌리섞음병원균의 진단을 위한 RT-PCR KIT의 개발

도은수 한국자원식물학회 2003 한국자원식물학회지 Vol.16 No.1

C. destructans는 인삼에서 가장 문제가 되고 있는 뿌리섞음병을 유발하는 매우 중요한 미생물이다. 현재까지 정상적인 인삼포장이나 폐포지에서도 이 병원균의 농도를 조사할 만한 방법이 없어 이를 쉽게 조사함으로서 인삼 예정지 관린시 도움을 줄 수 있는 새로운 방법이 절실이 요구되고 있다. 본 연구에서는 nested PCR이란 분자생물학적 방법을 이용하여 효과적으로 매우 낮은 농도의 C. destructans을 검출할 수 있는 방법을 개발하였다. 2개의 universal ITS primers(ITS5F와 ITS4R)을 사 용 하 여 Cylindrocarpon spp.의 rDNA로부터 ITS영역을 증폭하였다. 이어 C. destructans의 specific primer(Nest 1 과 Nest 2)을 사용하여 최적의 PCR조건으로 재증폭시켜 밴드를 확인하였다. 또한 이런 2번의 과정을 4개의 primer를 동시에 사용함으로서 한번에 확인할 수 있는 방법을 개발하였으며 이에 따른 PCR조건도 확립하였다. 따라서 본 방법에 의해서 인삼포장의 토양에서 채취된 매우 낮은 농도의 wild type C. destructans spore로부터 성공적으로 positive band을 확인함으로써 추후 인삼포장의 선정 및 4년생에서 6년까지(홍삼포) 재배기간등의 예측에 활용 될 것으로 생각된다. Cylindrocarpon destructans is the major pathogen inducing the root rot disease in ginseng. Up to now, there is no reliable and convenient method to analyze the spore density or population of this pathogen in ginseng-growing soil or any contaminated farmlands. Therefore, it will be very valuable to develop a new and reliable method in detecting the spore of this pathogen. In this study, a molecular biological technique using two step nested PCR method, was developed. Two universal ITS primers, ITS5F and ITS4R were used in the first round of PCR to amplify a fragment of ITS region from the genomic DNA of C. destructans. The specific prmers Nest 1 and Nest 2 were designed and used in the second round of PCR to amplify a inner fragment from the first round PCR product of C. destructans. C. destructans spore, only soil samples from the diseased ginseng farm produced the positive bands, suggesting its usefulness in detecting the C. destructans spores in soil samples. Thus it is recommended to first extract the whole genomic DNA from soil samples and use it for the PCR reaction, thereby eliminating the inhibitory activity of soil components.

사과겹무늬썩음병균에 대한 몇가지 약용식물 추출액의 항균활성

도은수 중부대학교 자연과학연구소 2000 自然科學硏究論文集 Vol.9 No.1

Crude extracts from 3 medicinal plants were evaluated for their antifungal activity against B. dothidea. In addition, the extracts were applied to apples to investigate the usefulness for disease control. The results were summarized as follows; Among the crude extracts from 3 species being tested, it of Anemarrhena asphodeloides showed considerably high antifungal activity, it of Coptis japonica exhibited strong antifungal activity and it of Phellodendron amurense also demonstrated considerable antifungal activity against B.dothidea, respectively. The crude extracts extracted with methyl alcohol had better antifungal activity than those of extracted with water at either room temperature or boiling condition. Antifungal activity of three crude extracts lasted longer in a refrigerator than in the room temperature when the extracts were stored. Antifungal activity of three crude extracts to B.dothidea gradually were decreased with prolonged storage.

파검은무늬병균에 대한 지모, 황백나무 및 황련추출액의 항균활성

도은수 中部大學校 自然科學硏究所 1998 自然科學硏究論文集 Vol.7 No.1

3종의 약용식물 추출액을 공시하여 Alternaria porri에 대해 항균 활성 검정 및 파 검은무늬병에 대한 방제 효과를 조사한 결과는 다음과 같다. 1. Aasphodeloides의 물추출액은 추출원액처리시에도 거의 항균활성은 없었고, C.japonica와 P.amurense의 물추출액은 50%의 희석농도까지는 항균활성을 가지는 것으로 나타났다. A.asphodeloides의 MeOH 추출액은 배지내의 희석농도가 1%일때 42.9% P.amurense의 MeOH 추출액은 0.2%의 희석농도에서 66.1%의 억제효과가 있었으며, C.japonica의 추출액은 0.01%의 희석농도에서도 59.2%의 억제효과가 있었다. 2. 3가지 추출액 모두 A.porri의 분생포자발아에 대한 억제효과는 매우 낮았으나, 추출액 처리시에 발아관의 신장은 상당히 억제되는 경향이었다. 3. Methyl alcohol로 추출한 식물체의 조추출액이 물을 용매로 하여 상온 추출하거나, 끓여서 추출한 조추출액 보다 항균활성이 우수한 것으로 나타났다. 4. A.asphodeloides 추출액의 경우 저장기간과 관계없이 항균활성이 낮았고, P.amurense와 C.japonica의 추출액은 저장기간이 길어짐에 따라 항균활성이 감소하는 경향이었다. 5. A.asphodeloides의 추출액은 방제효과가 낮았으나, P.amurense 및 C.japonica는 추출원액을 처리했을 때 100%의 방제효과가 있었다. 0.2%의 희석농도에서도 각각 75.0% 및 87.5%의 방제효과가 있었고, 특히 C.japonica의 추출액은 0.05%의 희석농도에서도 50.0%의 방제효과가 있어 3가지 추출액 중 가장 효과가 있었다. 6. 파잎에는 추출액에 의한 어떠한 약해의 증상도 나타나지 않았다. Crude extracts from three medicinal plants were evaluated for their antifungal activity against Alternaria porri. In addition, the extracts were applied to welsh-onion to investigate the usefulness for welsh-onion alternaria leaf spot control. The results were summarized as follows; Of the crude extracts, three medicinal plants it of A. asphodeloides was not showed antifungal activity, but them of C.japonica and P.amurense exhibited strong antifungal activity against A.porri. Conidiosporial germination of A.porri was not inhibited but elongation of germ tube was inhibited by the application of three medicinal plant extracts. Methyl alcohol extract had better antifungal activity than water extract at either room temperature or boiling condition. Antifungal activity of three medicinal plant crude extracts against A.porri gradually was decreased with prolonged storage. Control effect of crude extract of P.amurense and C.japonica was excellent, they indicated perfect conrol value at 1.0% concentration, and P.amurense showed 75.0%, and C.japonica 87.5% control value at 0.2% concentration, respectively, and C.japonica showed 50.0% control value at 0.05% concentration against welsh-onion alternaria leaf spot(A.porri). Extract of C.japonica of to the three medicinal plants was superior the others in control effect against welsh-onion altenaria leaf spot. Phytotoxic symptoms were not observed in welsh-onion leaves with exogenou foliage application of the three medicinal plants crude extract.

TIBA 處理가 大豆의 生育과 收量에 미치는 影響

都銀洙,梁啓鎭,金應吉 中部大學校 農業開發硏究所 1994 農業開發硏究論文集 Vol.3 No.-

無限伸育形인 無限콩과 有限伸育形인 普光콩을 試驗 材料로하여 摘芯과 TIBA(2, 3, 5-triiodobenzoic acid) 處理가 生育과 收量에 미치는 影響을 究明코자 本 試驗을 實施하였던 바 그 結果를 要約하면 다음과 같다. 摘芯과 TIBA 處理에 依하여 莖長과 主莖節數가 모두 減少되었고 減少의 폭은 無限콩이 普光콩보다 컸다. 節間長은 摘芯으로 길어졌으나 第 5葉期의 TIBA 處理에서는 7~10번째 節間長이 特히 짧아져 莖長이 단축되었다. 摘芯과 TIBA 處理는 主莖節數와 粒數를 減少시킨 반면에 分枝莢數와 粒數는 增加되었다. 收量은 普光콩에서는 增收되지 않았으나 無限콩에서는 摘芯區에서 9%, TIBA 處理區에서 13% 增收되었다. This experiment was conducted to investigate the effects of pinching off the terminal bud and TIBA(2, 3, 5-triiodobenzoic acid) on growth and yield characters of soybean. The results were summarized as follows ; Number of nodes and length of main stem were decreased by pinching and TIBA and degree of reduction was larger for Muhankong, a Indeterminate variety, than Bokwangkong, a determinate variety. Internode length was increased by pinching, but decreased by TIBA treatment. Especially, the lengths of the 7th~l0th internodes were decreased significantly by TIBA at V_6 stage. Numbers of pods and seeds on main stem were decreased, but numbers of pods and of seeds on branches were increased by pinching and TIBA treatment. Yield was not affected by pinching and TIBA in Bokwangkong, but soybean yield was increased by pinching and TIBA treatment, 9% and 13%, respectively, in Muhankong.

지모추출액의 항균활성과 항균성물질의 동정

도은수 中部大學校 自然科學硏究所 1999 自然科學硏究論文集 Vol.8 No.1

A.asphodeloides 추출액을 공시하여 Phytophthora capsici. Fusarium oxysporum, Botrytis cinerea, Botryosphaeria dothidea, Colletotrichum gloeosporioides, C.truncatum과 Alternaria porri에 대해 항균 활성 검정, 몇가지 작물에 대한 병해 방제 효과를 조사 그리고 항균 활성 물질을 동정한 결과는 다음과 같다. 1. 지모의 추출액은 P.capsici, C.gloeosporioides 및 B.dothidea에 대해서 항균력이 우수하였다. 2. Methanol을 용매로 추출하여 분리된 물질과 saponin의 항균활성을 비교할 때, 분리된 물질은 균주에 따라서는 saponin표준품 보다 우수한 항균력을 나타내는 것이 있었다. 3. 고추 역병, 참깨 시들음병 및 고추 탄저병에 대해서 방제효과가 인정되었다. 4. 고추와 참깨는 수경재배형식의 유묘검정에서는 약해가 있었으나, pot시험에서는 약해가 없었다. 또한 고추 및 참깨의 종자에 조추출액을 처리했을 때 발아가 저해되거나 유근의 신장이 억제되는등 약해가 유발되었으나, 고추 및 파의 입과 고추 과실에 조추출액을 처리하였을 때에는 아무런 해가 나타나지 않았다. 5. 지모의 조추출액으로부터 연한 갈색분말 5.67g/100g을 얻을 수 있었다. 이들 물질을 HPLC로 분석한 결과 항균성을 나타내는 물질은 saponin으로 동정되었다. Crude extract of Anemarrhena asphodeloides was evaluated for their antifungal activity against Phytophthora capsici. Fusarium oxysporum, Colletotrichum gloeosporioides, C.truncatum, Botrytis cinerea, Botryosphaeria dothidea and Alternaria porri and antifungal active compound from the extract was identified. In addition, the usefulness of the extract for some plants disease control was investigated. The results were summarized as follows: Crude extract of A.asphodeloides exhibited antifungal activity against P.capsici, F.oxysporum, C.dematium and B.dothidea. Antifungal activity of the substance isolated from A.asphodeloides was similar to a standard saponin, but was superior to it of a standard saponin against some pathogens. Red-peper fruit rot, sesame stem rot and red-peper anthracnose were effectively controlled by the crude extract of A.asphodeloides. Phytotoxicity was not observed in the red-pepper and welsh-onion leaves and red-pepper fruits with exogenous foliage application of the crude extract. Seed germination and radicle growth of red-pepper and sesame were inhibited by the crude extract of A.asphodeloides. 5.67g of light brownish compound per 100g of A.asphodeloides was obtained and it was identified as saponin by HPLC.

몇가지 약용식물 추출액에 의한 참깨 시들음병의 방제

도은수 中部大學校 農業生命資源科學硏究所 1998 農業生命資源科學硏究論文集 Vol.6 No.-

3종의 약용식물 추출액을 공시하여 Fusarium oxysporum에 대해 항균 활성 검정, 참깨에 대한 병해 방제 효과 및 약해를 조사한 결과는 다음과 같다. 1. C.japonica P.amurense 추출액은 배지내의 희석농도가 1%일 때 균사생장을 완전히 억제하였으며 0.2%의 희석농도에서도 50% 이상의 균사생장 억제효과가 있었으나 그 이하의 희석농도에서는 억제효과가 낮았다. A.asphodeloides 추출액은 1%의 희석농도에서 균사생장을 약간 억제하였다. 2. C.japonica 추출액은 1%의 희석농도로 처리하여도 90% 이상의 Fusarium oxysporum 분생포자의 발아 억제효과가 있었으나, A.asphodeloides와 P.amurense 추출액 처리에 의한 발아 억제효과는 매우 낮았다. 반면에 3가지 추출액의 저농도처리에서도 후막포자 발아가 억제되었다. 3. A.asphodeloides와 C.japonica 추출액의 경우 저장기간이 길어짐에 따라 항균력이 감소하는 경향이었으나, P.amurense 추출액은 저장기간이 길어져도 항균력에는 차이가 없었다. 4. Methanol에 의한 추출액은 1%의 희석농도로 처리하였을 때 A.asphodeloides 추출액은 87.9%, C.japonica 및 P.amurense 추출액은 각각 97.6% 및 92.0%의 발병 억제효과가 있었으며, 특히 C.japonica 추출액은 저농도에서도 방제 효과가 우수하였다. 5. 유묘에 대해서 수경재배법으로 검정했을 때에는 어느 정도의 약해가 유발되나, pot시험으로 토양에 처리 할 때는 약해가 없거나 아주 경미하였다. 또한 참깨 종자에 추출액을 처리하였을 경우 A.asphodeloides와 C.japonica 추출액의 100%액 처리에 의해 발아가 상당히 억제되었으나, P.amurense 추출액은 어느 희석농도에서도 발아에 영향을 주지 않았다. 그러나 세가지 추출액 모두 어느 희석농도에서나 유근의 신장을 억제시켰으며, 특히 C.japonica 추출액 처리시 유근의 신장은 거의 이루어지지 않았다. Antifungal activities of the crude extracts of Anernarrhena asphodeloides, Coptis japonica and Phellodendron amurense were tested against Fusarium oxysporum, and the control effect to sesame stem-rot and phytotoxity to sesame were investigated. The results were summarized as follows; Mycelial growth of F.oxysporum was inhibited by methanol extracts of C. japonica and P. amurense strongly but it of A. asphodeloides weekly. Conidial germination of F.oxysporum was inhibited above 90% by 1% concentration application of water extract of C.japonica, but wes very weekly inhibited by the extracts of A.asphodeloides and P.amurense, but germination of chlamydospores was effectively inhibited at the low concentration of 3 medicinal plant extracts. Antifungal activity of 3 medicinal plant extracts against F.oxysporum were gradually decreased with prolonged storage period. Sesame stem-rot was effectively controled by the methanol extracts of 3 medicinal plants. Of these, the extract of C.japonica was most effective to the control of sesame stem-rot caused by F.oxysporum, even at a 0.2% concentration. Sesame seedling was showed a phytotoxicity by water cultural method, but was not by pot test. Seed germination and radicle growth of sesame were inhibited by the application of water extracts of 3 medicinal plants.

땅콩莢에서 分離한 Rhizoctonia solani 의 系統 및 特性

都銀洙 건국대학교 1990 論文集 Vol.30 No.1

This experiment was conducted to study the possibility of grouping based on the cultural characteristics and pathogenecity of Rhizoctonia solani isolated from peanut pod. The results were as follows ; 1. The optimtum temperature for colony growth of each strains was 30℃. 2. The optimum pH for colony growth of each strains pH 6-8. 3. In the effect of nitrogen source on colony growth, SII - B strain was poor, SI moderate, and SII-A, SIII-A and SIII-B were best. And in carbon source, SII -B strain was poor, SI moderate, SII- A good, and SIII-A and SIII-B were best. 4. In pathogenecity of 5 strains, SI, SIII-A and SIII-B were severe pathogenic and SII-A and SII-B Pathogenic. 5. R. Solani isolated from peanut pod was divided three group(4 types 5 strains) from above results.

고려 인삼의 유네스코 세계 인류무형문화유산 등재

도은수 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1