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답 다년생 잡초방제에 관한 연구 1 : 추경이 다년생잡초의 방제에 미치는 영향
金吉雄,崔鉉玉 韓國作物學會 1976 Korean journal of crop science Vol.21 No.1
다년생 잡초의 지하번식기관의 토양중 분포조사와 추경심도 및 AVIROSAN에 의한 다년생 잡초 가래 방제에 미치는 영향에 대하여 얻어진 예비결과를 요약하면 다음과 같다. 1. 너도방동산이와 올미의 괴경은 주로 0∼5cm의 깊이에 얕게 분포하여 있으나 가래의 인경과 올방개의 괴경은 각각 87%, 65%가 주로 10cm 이상의 깊이에 분포하였다. 2. 추경 20cm는 무경운, 무제초에 비하여 약 63%나 가래의 발생을 적게 하였다. 3. AVIROSAN 10a당 3kg 처리는 가래 방제에 특효하였으며 수량도 손제초 2회에 비하여 4% 증수되었다. 4. 보리짚 10a 당 250kg 처리도 가래의 발생억제는 물론 손제초 2회보다 1% 증수한 결과를 보였다. Distribution of underground organs of perennial weeds in soil and ecological control measure were evaluated. Tubers of Cypers serotinus and Sagittaria pygmaea were mostly presented in a shallow depth of 0 to 5cm, however, Potamogeton distinctus's bulbs and Eleocharis kuroguwai's tubers were distributed in the soil at 10 to 20cm. A 20cm autumn plowing was, to some extent, effective to reduce Potamogeton distinctus. The herbicide, avirosan at 3kg/10a alone, was extremely effective in controlling Potamogeton distinctus.
雜草防除試驗 遂行方法 및 評價法 : 圃場試驗을 中心으로 Emphasis on the Field Trials
金吉雄 慶北大學校 새마을硏究所 1979 農村과 科學 Vol.2 No.-
This paper attempted to outline the evaluation methods conducting weed control research. It is essential for well designed, carefully prepared and accurately handled experiments to obtain the meaningful results. The purformance of a herbicide can be greatly affected by a number of factors, particularly environmental factors such as rainfall, light intensity, soil texture, % organic matter, and soil moisture etc. After establishment of weed control trials and the treatments applied it is important to precisely and regularly assess the effects of the treatments on the crops and the weeds. The methods of weed assessment like visual weed ratings, weed number counts, weed height and weed dry weight, and crop assessment such as visual estimation, stand counts, stand height, crop appearence, yield and crop quality were described and discussed. Interpretation of results should be based on economic analysis, considering all factors affecting the purformance of a herbicide.
김길웅,유병제 대구대학교 기초과학연구소 1984 基礎科學硏究 Vol.1 No.-
Several proteins were detected in the muscle-conditioned medium strongly suggesting that the cells in cultured released polypeptides which might act on the membrane of neighboring cells to initiate the fusion. The muscle-conditioned mediums of fusion arrest conditions were analyzed. It was showned that the muscle-conditioned medium promotes the muscle cell fusion.
목화자엽에 함유된 Glycosidase들의 생리학적 역할에 관한 연구 : 목화자엽에 함유된 α-Mannosidase의 특성
김길웅,이갑숙,이조광 대구대학교 산업기술연구소 1987 産業技術硏究 Vol.6 No.-
목화자엽의 glycosidase들 가운데 α-mannosidase의 특성과 생리적 기능에 대하여 연구하였던 바, 효소의 최적 pH는 3.8과 4.2로 나타났으며 K_(m)값과 V_(max)값은 각 각 3.70mM과 0.66 ㎛/mg/min 이었다. 또한 α-mannosidase는 기질에 대한 특이성을 가지고 있어 p-nitrophenyl-α-D-mannopyranoside, Xanthan, Soybean lectin 등을 가수분해 시켰다. Cotton α-mannosidase의 특성은 Jack bean meal의 것과 매우 비슷하여 Ovalbumin과 Soybean lectin과 같은 glycoprotein으로부터 mannose 를 분리시킬 수 있었다.
Clofibrate(CPIB)로 飼育한 家兎의 血淸 脂質濃度에 관한 硏究
金吉雄,趙萬熙 조선대학교 기초과학연구소 1979 自然科學硏究 Vol.2 No.1
This study was undertaken to determine the effect of serum lipid in rabbits fed with clofibrate diet. The five group of rabbits were fed with clofibrate, which analyzed free cholesterol easter, triglyceride and phospholipid for ten fays. The results were as follows : 1. There was no large difference between the serum lipid levels in gthe group fed with clofibrate 0.1㎎/day and control group in rabbits. 2. The serum lipid levels in the group fed with clofibrate 0.2㎎/day was as a result of free cholesterol 17.0±2.1 cholesterol ester 37.8±4.0 phosphlipid 47.2±3.3, triglyceride 73.1±3.6㎎/100㎖ in rabbits. 3. The serum lipid levels in the groups fed with clofibrate 0.3㎎/day was a result of free cholesterol 15.4±2.4, cholesterol ester 36.8±3.3, phospholipid 39.4±3.4, triglyceride 67.7±3.5㎎/100㎖ in rabbits. 4. The serum lipid levels in the groups fed with clofibrate 0.4㎎/day was as result of free cholesterol 14.2±2.3, cholesterol ester 33.6±4.8 Phospholipid 32.3±2.8, triglyceride 58.9±3.5㎎/100㎖ in rabbits. 5. From the above results we obtained the Normal serum lipid levels in rabbits could be confirmed by free cholesterol 20.0±2.3, cholesterol ester 41.9±4.8, phosphlipid 53.7±3.6 and triglyceride 78.4±2.6㎎/100㎖ in rabbits.
김길웅,박용구,최명석 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Anthocyanin formation in callus cultures using Populus albs × Populus glandulosa was evaluated on basal MS medium supplemented with various levels of growth regulators, sucrose and nitrate concentrations. The highest yield of anthocyanin from cultured cells was produced under 5% sucrose, 1/8 strength of nitrate(12.5% of basic concentration) and combination of 1.0 ㎎/ℓ IAA with 2 ㎎/ℓ BAP, respectively. The high anthocyanin producing cell line no. 11 was selected among 15 cell lines, showing over 80% cells contained anthocyanin producing cells. From these cells, the highly productive red protoplast was isolated and the highest protoplast yield, 6.7 × 10^6 was obtained in enzyme combination IV which is composed of 2.0% cellulase, 0.5% macerozyme and 0.1 % pectolyase.