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NMED-01과 NMED-02의 간기능 개선에 대한 인체 효능 평가
박상욱 ( Sangwoug Park ),권용범 ( Yong Beom Kwon ),김기태 ( Ki Tae Kim ),신선미 ( Seon Mi Shin ),임강현 ( Kang Hyun Leem ),고흥 ( Heung Ko ),송미경 ( Mi Kyung Song ),정윤철 ( Yoon Chul Jung ),김호철 ( Ho Cheol Kim ),박주연 ( Ju Y 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6
Objectives: The purpose of this study was to determine whether NMED-01 or NMED-02 improves laboratory test results in participants with liver function disorder. Methods : This is a randomized, placebo-controlled trial in which participants, treating physicians and data management staff were blinded to treatment group. The study was conducted at Semyung university oriental medicine hospital in Jecheon where participants with high level of serum γ-GTP (60-350 U/L) were enrolled. The intervention consisted of three times daily ingestion of either two capsules of placebo, NMED-01 (NeuMed. co. ltd., Seoul), or NMED-02 (NeuMed. co. ltd., Seoul) for twelve weeks. To evaluate the efficacy and safety of NMED-01 and NMED-02, we primarily evaluated the degree of decrement of serum γ-GPT level among three groups. Secondarily the decrement of serum ALT, AST, and triglyceride level in each group were also evaluated. Adverse effects were monitored during the twelve weeks treatment. Results : The change of γ-GTP level of NMED-01 group was lower than that of placebo group at the end of 12-week administration (28.1±38.7 U/L vs. 9.3±27.0 U/L, p=0.046). Other variables including AST, ALT, and triglyceride level were not significantly reduced. The decrement of γ-GPT, AST, ALT, and triglyceride level of NMED-02 group was not significant. There were no significant adverse effects or toxicities during treatment period. Conclusions : Participants receiving NMED-01 had improvement in laboratory test results. Despite a modest sample size, our results suggest that NMED-01 are safe and may be potentially effective in improving liver function. However, NMED-02 have lack of a detectable effect in this study.
붉은싸리버섯 추출물의 항산화 및 Human Neutrophil Elastase 저해활성
김관철 ( Kwan-chul Kim ),권용범 ( Yong-beom Kwon ),장해동 ( Hae-dong Jang ),김재화 ( Jae Wha Kim ),정재철 ( Jae Cheol Jeong ),이익수 ( Ik-soo Lee ),하병조 ( Byung-jo Ha ),유익동 ( Ick-dong Yoo ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.3
천연자원으로부터 항노화 화장품 신소재를 탐색하던 중, 국내 자생버섯의 일종인 붉은싸리버섯 자실체 추출물이 항산화 활성과 인체 호중구 엘라스타제 저해활성이 우수함을 확인하고 일련의 연구를 수행하였다. 붉은싸리버섯 추출물의 DPPH 라디칼 소거활성은 붉은싸리버섯 추출물 500 μ g/mL 처리시 117.0 mg/mL (ascorbic acid 환산값)의 매우 우수한 소거활성을 나타냈다. Peroxy 라디칼 소거활성을 oxygen radical absorbance capacity (ORAC) assay 를 통하여 측정한 결과 붉은싸리버섯 추출물 1, 10, 20 μ g/mL 처리 시, 각각 0.8, 5.2, 7.8 ORAC<sub>Roo</sub> (trolox equivalents, 1 μ M)로 농도 의존적으로 높은 소거활성을 나타냈다. 뿐만 아니라 cellular antioxidant capacity를 DCF fluorescence intensity (% of control)로 조사한 결과에서도 붉은싸리버섯 추출물 20 μ g/mL 처리시 약 30% 이상 높은 항산화 활성을 나타냈다. Human neutrophil elastase 저해활성은 농도 의존적으로 저해활성을 나타냈으며 특히 에탄올 추출분획에서 ED<sub>50</sub> 값은 42.9 μ g/mL이었다. 붉은싸리버섯 추출물은 Bacillus subtilis (B. subtilis), Escherichia coli (E. coli), Candida albicans (C. albicans), Aspergillus oryzae (A. oryzae) 균주 모두에서 항균활성은 나타나지 않았다. 또한 염증성 cytokine인 interleukin-10 및 interferon-γ (IFN-γ)의 생산 또는 분비 조절에는 영향을 미치지 않았다. 이상의 결과로 붉은싸리버섯 추출물은 항산화활성과 elastase 저해활성을 우수하여 피부에 자극이 없는 항노화 화장품 조성물로 유용하게 사용될 수 있음을 확인하였다. In searching for novel agents for skin anti-aging from natural resources, we found that the extract of the fruiting bodies of Ramaria formosa (R. formosa) had significant antioxidant and human neutrophil elastase (HNE) inhibitory activities. R. formosa extract exhibited a considerable DPPH radical scavenging activity with an antioxidant content of 117.0 mg/mL (ascorbic acid equivalents) at the concentration of 500 μg/mL. The capacity of R. formosa extract to scavenge peroxy radicals measured by ORAC assay also showed dose-dependent antioxidant effect with ORAC<sub>Roo</sub> (trolox equivalents, 1 μM) values of 0.8, 5.2, and 7.8 at the concentrations of 1, 10, and 20 μg/mL. The cellular antioxidant capacity of R. formosa extract was investigated by assaying the cellular fluorescence intensity using dichlorodihydrofluorescein (DCF). The cellular oxidative stress induced by AAPH, Cu<sup>2+</sup> or H2O2 in HepG2 cells was significantly attenuated by more than 30% at 20 μg/mL of R. formosa extract. HNE activity was reduced by treatment with R. formosa extract in a dose-dependent manner, and the ED<sub>50</sub> value for the ethanol extract of R. formosa was 42.9 μg/mL. R. formosa extract did not exhibited antimicrobial activity against four microorganisms including Bacillus subtilis (B. subtilis), Escherichia coli (E. coli), Candida albicans (C. albicans), Aspergillus oryzae (A. oryzae). Furthermore, the extract did not affect the inflammatory cytokine production of interleukin-10 and interferon-γ in NK92 cells. From the above results, we found that R. formosa extract has considerable antioxidant and elastase inhibitory effects, and does not stimulate immune cells. These findings suggest that R. formosa extract may be used as a bioactive component in cosmetic composition.