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항생물질 생산 토양 Actinomycetes 균주 선별과 항생물질 생산특성 조사
구양모(Yang Mo Goo),이윤영(Youn Young Lee),정연숙(Youn Sook Chung),이영복(Young Bok Lee),조영애(Young Ae Joe),조희영(Hee Yeong Cho),고영선(Young Sun Koh),이창훈(Chang Hoon Lee) 대한약학회 1991 약학회지 Vol.35 No.3
Selective culture of actinomycetes from soil microbes and their antibiotic producing characters by agar-disk method were examined. Some of the organisms which produced antibiotics on agar disk did not produce antibiotics in liquid culture. Further examination indicated that production of antibiotic was dependent on the composition of medium. Many streptomycestes produced antibacterial substances in tryptic soy broth but others produced antifungal antibiotics in V-8 broth. Production of antibacterial substances by Streptomyces sp. was also dependent on the medium composition.
항생물질을 생성하는 토양균의 대량 검색을 위한 새로운 방법
구양모(Yang MoGoo),이윤영(Youn Young Lee),조영애(Young Ae Joe),이영복(Young Bok Lee),정원윤(Won Yun Chung),이창훈(Chang Hoon Lee),고영선(Young Sun Koh),조희영(Hee Yeung Joe),정연숙(Youn Sook Chung) 대한약학회 1991 약학회지 Vol.35 No.4
A new agar-disk method was developed from the fast selection and characterization of antibiotic producing organisms.
이영복,구양모,Lee, Young-Bok,Goo, Yang-Mo 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4
$\alpha$-Aminophenylacetonitrile의 농도를 브롬을 가한 강산과 함께 흔들어 주어 생성되는 cyanogen bromide를 분석하여 이루는 정량방법을 조사하였다. Cyanogen bromide는 pyridine과 함께 반응시킨 후에 benzidine을 처리하면 530 nm에서 최대의 흡광을 갖는 화합물이 얻어진다. 이 흡광은 0.01-0.2 mM의 농도 범위에서 $\alpha$-aminophenylacetonitrile의 농도에 대하여 비례적으로 증가하였다. 시료에 2-mercaptoethanol, 또는 phenylglycine을 가하여 주어도, 붉은 색의 생성에 큰 영향을 미치지 않았으나, 알부민이나 phenylglycinamide가 시료에 존재하는 경우 530 nm에서 흡광이 이들의 농도에 따라 직선적으로 증가하였다. 황화합물들인 cysteine, methionine, glutathione과 thioglycolic acid는 $\alpha$-aminophenylacetonitrile의 분석에 관여하지 아니하였다. 여러 형태의 생물학적인 시료내에 $\alpha$-aminophenylacetonitrile의 농도 정량에서 정밀도를 조사하여 보았다. Quantitative analysis of $\alpha$-aminophenylacetonitrile was examined by analyzing cyanogen bromide formed by incubation of a sample in strong acidic solution with bromine. The reaction of the cyanogen bromide with pyridine followed by benzidine gave a substance showing a maximum absorption at 530 nm. The intensity of this absorption was linearly increased with concentration of $\alpha$-aminophenylacetonitrile in the range of 0.01-0.2 mM. Addition of mercaptoethanol, or phenylglycine in the sample did not affect so much on colorization, but when albumin or phenylglycinamide was existing in the solution, the absorption at 530 nm was found linearly increased with its concentration. Sulfur compounds, cysteine, methionine, glutathione and thioglycolic acid did not interfere in the analysis of $\alpha$-aminophenylacetonitrile. Accuracy of the analytical methods for $\alpha$-aminophenylacetonitrile in various biological samples were further examined.
아미노페닐 아세토나이트릴의 정량 분석 : 가수분해 산물들 , 단백질 , 유화수소화합물들과 생체 시료들이 분석에 미치는 영향
이영복,구양모 ( Young Bok Lee,Yang Mo Goo ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4
Quantitative analysis of α-aminophenylacetonitrile was examined by analyzing cyanogen bromide formed by incubation of a sample in strong acidic solution with bromine. The reaction of the cyanogen bromide with pyridine followed by benzidine gave a substance showing a maximum absorption at 530 ㎚. The intensity of this absorption was linearly increased with concentration of α-aminophenylacetonitrile in the range of 0.01-0.2 mM. Addition of mercaptoethanol, or phenylglycine in the sample did not affect so much on colorization, but when albumin or phenylglycinamide was existing in the solution, the absorption at 530 ㎚ was found linearly increased with its concentration. Sulfur compounds, cysteine, methionine, glutathione and thioglycolic acid did not interfere in the analysis of α-aminophenylacetonitrile. Accuracy of the analytical methods for α-aminophenylacetonitrile in various biological samples were further examined.