임플라논 소실후 정상자연분만 1예

강혜심,김성엽 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

선문과 회향

강혜 동국대학교 석림회 2005 석림 Vol.39 No.-

Vero Cell-Conditioned Medium에서의 배양이 생쥐 후기 2-세포기 배아 발생에 미치는 영향

강혜나,하종식 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

본 실험결과 Vero cell과의 공동배양과 Vero cell-conditioned medium에서의 배양은 생쥐 후기 2-세포기 배아의 부화율을 높이고 세편이 적은 양질의 배아획득을 돕고 세포 증식을 빠르게 해 팽윤 배반포시 할구수를 증가시키는 것으로 나타났다. 그러나 앞으로 공동배양 기작에 작용할 것으로 보이는 embryotrophic factor나 배양액에서 공동배양 세포주에 의해 제거되는 발생저해 물질 등의 분자생물학적 연구가 진행되어야 하고, 수정시 정자등이 공동배양 세포주의 monolayer에 결합하여 수정 기회를 감소시켜 여러 장점에도 불구하고 오히려 수정률을 감소시킬 수 있는 직접적인 공동배양보다 conditioned medium에서의 배양에 관심을 가져야할 것으로 본다. This study was conducted to evaluate the ability of Vero cell-conditioned medium for supporting mouse embryo development in vitro. The mouse late 2-cell embryos were cultured in control media(Ham's F-10+10%FBS), media with Vero cell monolayer and Vero cell-conditioned media for 4 days and measured the hatching rate and cell number in the blastocyst stage. The hatching rate in experimental groups was increased significantly compared with embryos in control group(p<0.01). On the other hand, the degree of blastomere fragmentation exhibited a opposite trend to that of the developmental capacity(P<0.05). And also the cell numbers of expanded blastocysts in experimental groups were increased significantly compared with the control group(p<0.001). There was, however, no difference between experimental groups. These results indicate that Vero cell-conditioned medium supported the mouse embryo development as a Vero cell monolayer. And the mechanism for enhancement of the development potential of embryos may be releasing the embryotrophic factor during the medium-conditioning period.

Vero Cell과의 공동배양이 체외에서 생쥐 난자성숙과 배아발생에 미치는 영향

강혜나,하종식 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

Assisted reproductive technology(ART)have contributed significantly to alleviating subfertility in the childless couple. In spite of the many advances in the field of ART, the pregnacy and take-home baby rates for in virto fertilization(IVF)have been very poor. In order to overcome these problems, a variety of coculture systems has been devised. Vero cells, derived from African green monkey kidney, were selected because kidney and genital tract have a common embryonic origin. In addition, these cells are safe for coculture with embryos:they are highly controlled for viruses and other contaminants because they are used for vaccine production. Several investigators showed that coculturing human embryos with Vero cells in vitro resulted in an improvement of embryo development. However, they did not observe the same results using mouse oocytes and embryos. We thus designed a series of experiments to demonstrate whether or not Vero cells do indeed enhance mouse oocyte maturation and embryo development. In this experiment, Vero cell does not allow the mouse immature oocytes to be enhanced maturation rate in vitro. To study the 'In-Vitro 2-Cell Block' in mouse embryo, we have cocultured ICR one-cell mouse embryos with Vero cell in different medium. In Ham's F-10 the mouse embryos arrested their development prior to 4-cell stage(control 76.7%:coculture 75.0%). In contrast, the coculturing mouse embryos revealed enhanced development(control 0%;coculture 22.8%) in human tubal fluid(HTF)only in late embryonic stages(hatching). On the other hand, the degree of blastomere fragmentation exhibited a reverse trend to that of the developmental capacity. Embryos from coculture groups(Ham's F-10 & HTF)showed some fragmentation(0% & 4.2%)while 13.3% and 14.3% of the embryos in control groups(Ham's F-10 & HTF)were severely fragmented (P<0.05). Thus the use of coculture systems appears to be dependent on the type of medium used as a support. The development rate of late 2-cell mouse embryos in Vero cell coculture was no significant differences until blastocyst stage but improved at late develpmental stage(control 42.1% : coculture 70.7%). Thus the Vero cell coculture system was shown to increase the hatching rate of mouse embryos.

Vero Cell과의 공동배양이 체외에서 생쥐 난자성숙과 배아발생에 미치는 영향

강혜나,하종식 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Assisted reproductive technology(ART) have contributed significantly to alleviating subfer-tility in the childless couple. In spite of the many advances in the field of ART, the pregnancy and take-home baby rates for in vitro fertilization(IVF) have been very poor. In order to overcome these problems, a variety of coculture systems has been devised. Vero cells, derived from African green monkey kidney, were selected because kidney and genital tract have a common embryonic origin. In addition, these cells are safe for coculture with embryos : they are highly controlled for viruses and other contaminants because they are used for vaccine production. Several investigators showed that cocultureing human embryos with Vero cells in vitro resulted in an improvement of embryo development. However, they did not observe the same results using mouse oocytes and embryos. We thus designed a series of experiments to demonstrate whether or not Vero cells do indeed enhance mouse oocyte maturation and embryo development. In this experiment, Vero cell does not allow the mouse immature oocytes to be enhanced maturation rate in vitro. To study the 'In-Vitro 2-cell Block' in mouse embryo, we have cocultured ICR one-cell mouse embryos with Vero cell in different medium. In Ham's F-10 the mouse embryos arrested their development prior to 4-cell stage(control 76.7%;coculture 75.0%). In contrast, the coculturing mouse embryos revealed enhanced development(control 0%;coculture 22.8%) in human tubal fluid(HTF) only in late embryonic stages(hatching). On the other hand, the degree of blastomere fragmentation exhibited a reverse trend to that of the developmental capacity. Embryos from coculture groups(Ham's F-10 & HTF) showed some fragmentation(0% & 4.2%) while 13.3% and 14.3% of the embryos in control groups(Ham's F-10 & HTF) were severely fragmented(P<0.05). Thus the use of coculture systems appears to be dependent on the type of medium used as a support. The development rate of late 2-cell mouse embryos in Vero cell coculture was no significant.

학생기록물에 대한 대학생의 인식 유형 연구 - 전남대학교를 중심으로 -

강혜라,장우권,Kang, Hye-Ra,Chang, Woo-Kwon 한국비블리아학회 2017 한국비블리아학회지 Vol.28 No.3

이 연구는 학생기록물의 효과적인 관리를 위해 주체적 생산자인 대학생의 인식에 대한 심층적인 연구가 필요함을 제기하고 Q 방법론을 통해 학생기록물의 중요도에 관한 주관적인 인식(Q1)유형과 학생기록물에 관한 주관적인 인식(Q2) 유형을 도출하는데 목적이 있다. 이를 위해 전남대학교의 학생을 대상으로 학년별로 8명씩 설문과 인터뷰를 진행한 결과, Q1 유형은 집단적 증거형, 주체적 활동형, 민주적 증거형, 성취 중시형이 도출되었으며, Q2 유형은 공동체 역사적 가치형, 개인적 소유형, 역사적 전문가 관리형, 역사적 활용 가치형, 비역사적 보존형이 도출되었다. 연구결과를 바탕으로 첫째, 다양한 기록물 속성을 포괄할 수 있는 관점에서 학생기록물의 관리방안의 수립이 필요하다는 점과 둘째, 공동체 아카이브의 관점에서 학생기록물의 관리방안이 수립되어야 한다는 점, 셋째, 학생들이 생산한 학생기록물의 보존을 위한 전략적인 방안(학생기록물의 생애주기를 고려한 보존을 위한 캠페인 등)이 필요함을 제안했다. This study brings up that further studies on the perceptions of university students should be conducted for efficient management of student records. Moreover, the Q methodology is used to identify subjective recognition (Q1) type related to the importance of student records and subjective perception (Q2) of student records type. For this purpose, I carried out questionnaires and interviews with eight students by each grade at Chonnam National University. As a result, Q1 type was derived from Collective Evidence type, Independent activity type, Democratic witness type, and achievement-oriented type. The type of Q2 is derived from Historical Value of Community Type, Personal Possession Type, Historical Expert Management Type, Value for Historical Practical Use Type, and Non-historical Preservation Type. Based on the research results, the following are suggested: First, the management of student records is needed in a way that encompasses various records of various records properties. Second, the management of student records should be established from the perspective of the Community Archives. Third, a strategic measure for the preservation of student records produced by students is necessary (e.g., a campaign for conservation of student records).

北宗禪硏究 - 頓漸辨證法的 止揚

姜慧? 東國大學校 釋林會 1976 석림 Vol.10 No.-

비강에서 발생한 악성 점막 흑생종 : 1례 보고 및 문헌 고찰 A case Report and Literature Review

강혜신,서현숙,전병훈,조혜제 인제대학교 1987 仁濟醫學 Vol.8 No.3

Primary malignant mucosal melanoma occurs very infrequently. The mucosa of the head and neck and vulvovaginal areas are most commonly involved. A case of primary malignant mucosal melanoma arising from the nasal cavity is presented and previous literatures are reviewed.

부산 동래의 역사지구 내 기존 골목길의 장소성을 통한 창업시설 계획안

강혜리(Kang, Hye-ri),이길임(Lee, Gill-im) 대한건축학회 2017 대한건축학회 학술발표대회 논문집 Vol.37 No.2

The spatial structure of the city is based on the flow of history, resulting in the cumulative nature of history and the presence of regional communities. The Dongnae area in Busan is experiencing a change of space by typing in different cities, leaving historical footprints disappear, increasing consumption space, and destroying the city’s identity. We intend to revive the historical site of the historical site and revive the establishment of the commercial community by restoring the integrity of the commercial community by restoring the community of shopkeepers.

속발성 무월경 외 비정상 표현형이 없는 Trisomy 8 Mosaicism의 증례 보고

강혜심(Hye Sim Kang),손영수(Young Soo Son),김성엽(Sung Yob Kim),박철민(Chul Min Park),심순섭(Soon Sup Shim) 대한의학유전학회 2011 대한의학유전학회지 Vol.8 No.1

체질성 8삼염색체 모자이시즘은 비교적 드문 염색체 이상으로 특이한 두개안면 모양 (변형 두개골, 돌출된 이마, 낮거나 변형된 귀), 골격계 기형, 심장 기형, 신장 기형, 잠복고환, 다양한 정도의 발달지체 등의 특징적인 표현형을 갖는다. 그 표현형과 세포유전학적 표현의 다양성 때문에 특정 질환이 생기기 전까지 진단이 늦어지기도 한다. 전형적인 표현형 없이 속발성 무월경을 동반한 28세 여성에서 발견된 체질성 8삼염색체 모자이시즘(47,XX,+8[9]/46,XX[41])을 보고한다. Constitutional trisomy 8 mosaicism (CT8M) is a relatively rare aneuploidy in humans with characteristic phenotypes including typical craniofacial feature (such as deformed skull, prominent forehead, low-set and/or dysplastic ears), skeletal malformation, cardiac anomaly, renal malformation, cryptochidism, varying degree of developemental delay. Due to the extremely variable phenotypic and cytogenetic expression, CT8M has gone undiagnosed in certain patients. We report a 28-year-old women with secondary amenorreha without characteristic CT8M phenotype. Chromosomal analysis showed a CT8M (47,XX,+8[9]/46,XX[41]).