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아밀로이드 베타 단백질에 의해 유도된 신경세포 독성에 대한 황정의 억제 효과 탐색
김은숙,최수진,강가혜,오명석,박우진,최영환,이철원,임학섭,조용권,문자영 대한암예방학회 2008 Journal of cancer prevention Vol.13 No.3
The amyloid β-protein (Aβ) is the principal component of the senile plaques characteristic of Alzheimer’s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify Aβ toxicity. In this study, we have investigated the protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on Aβ-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. For this study, we used MTT reduction assay for detection of protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on Aβ25-35-induced cytotoxicity to PC12 cells. We also used cell-based β-secretase assay system to investigate the inhibitory effect of organic solvent-extracts of Polygonatum odoratum on β-secretase activity. We previously reported that methanol extracts of Polygonatum odoratum strongly attenuated cytotoxicity induced by the three A fragments (Aβ25-35, Aβ1-42 Aβ1-43) to both SK-N-MC and PC12 cells. In the present study, we found that the organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol had the strong protective effects against Aβ25-35-induced cytotoxicity to PC12 cells and inhibitory potency to β-secretase activity. These results suggest that organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol may contain the protective component(s) against Aβ-induced cell death in PC12 cells as well as inhibitory component(s) to β-secretase activity. (Cancer Prev Res 13, 197-204, 2008)
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농특산품 전시판매장 디자인 현황 분석 및 유형별 분석 -농촌관광마을 현장조사 및 농업인 공급자 의식조사를 중심으로-
진혜련 ( Hye Ryeon Jin ),채혜성 ( Hye Sung Chae ),강가혜 ( Ga Hye Kang ),조록환 ( Lok Hwan Jo ) 한국농촌계획학회 2013 농촌계획 Vol.19 No.3
With the increase of visitors to rural tourism villages, the direct selling at sites is getting vitalized. Accordingly, their display stores is getting more important. Therefore, this study has selected 30 domestic rural tourism villages as study objects for the attitude survey of 200 farming suppliers and the site examination for the designs of those display stores in order to analyze their status and classify the types of necessity. Such operation status as sale item, sale method, method of supply and demand, major customer, sales scale, manager, opening hour, and operation cost were examined, to identity and for design factors the pattern, material quality and color were investigated. For the attitude of farming suppliers, the tactics of sales, the reason for being positive or negative, the functionality and the features of display stores were examined through brainstorming. IBM SPSS Statistics 20 Program was employed for Frequency, which indicated that village chiefs and store managers with the sales scale of 1 to 20 million won are dealing with female customers in their 40`s and 50`s and that those stores are open at the time of experience or year round without any operation expense. Permanent type and Fixed type were found to be the design factors of the display-case type with the material and the color of wood and orange respectively. The result of investigation analysis of farming suppliers` attitude showed the followings: the need of display stores is quite high, structure type and permanent type have high fitness and from the viewpoint of display-on-table type as a standard moving type was very convenient. The analysis of significant items at the characteristics of those display stores revealed that their locations, quality conservation, sanitation, users` convenience, designs and promotion are very important. The result of status analysis revealed that though there is a correlation among the types of display stores depending on the visiting season of tourists their installing is not desirable. Three types have been analyzed: Type 1 is a structure type only in the villages with continuous visitors, Type 2 a moving-table type only in the villages with temporary visitors and Type 3 is a fixed display-case type.
ScienceON
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