肝吸蟲症의 肝癌 誘發 可能性에 對한 發癌物質 3´-methyl-4-dimethylaminoazobenzene의 影響

閔弘基,鄭慶淑 梨花女子大學校 韓國生活科學硏究院 1986 韓國生活科學硏究院 論叢 Vol.37 No.-

原發性 肝癌 특히 膽管癌이 肝吸蟲症을 동반하고 있는 예가 간흡충증이 高率로 만연되고 있는 극동 및 동남아의 여러 지역에서 드물지 않게 발견되어 왔으며 이런 현상은 偶然의 合倂일 것이라고 주장되기도 하였으나 그 동안의 疫學的, 病理組織學的 및 實驗的 觀察로 제시된 결과는 이들 兩者 사이의 밀접한 相關性을 강력하게 시사하고 있다. 간흡충증으로 인하여 惹起되는 담관의 腺腫性 增殖과 담관상피세포의 粘液細胞化生은 動物宿主의 차이에 관계없이 나타나는 특징적인 병리조직학적 공통소견인 동시에 암으로 轉化될 수 있는 前殷階의 변화로 看做되고 있으나 간흡충감염만으로는 실험적으로 암을 유발시키지 못하며 某種의 發癌物質의 介入으로 이루어질 것이라는 의견이 支配的이다. 그러나 발암물질을 사용한 실험적 연구가 우리나라에서는 수행된 바 없었다. 본실험은 간흡충감염으로 유발되는 담관조직의 변화에 관한 根本 性狀을 해명하고 발암물징이 이에 미치는 영향을 규명하려는 노력의 一環으로 진행 되었다. 食品染料로 많이 쓰이고 있는 발암물질이며 axo화합물의 하나인 3‘-MeDAB을 간흡충감염 마우스에 투여함으로써 담관상피세포가 암세포로 轉化되어가는 移行期 所見을 경험할 수 있었으나 간흡충 또는 발암물질 단독투여의 경우에 있어서는 암발생 소견을 발견할 수 없었다. 이러한 결과를 근거로, 간흡충감염은 담관상피세포의 화생을 유발하는 先行的 要因의 役割을 하며 3‘-MeDAB은 화생된 세포를 자극하여 惡性化시키는 促進的 要因으로서 담관암 발생에 있어 相互 協同作用을 나타내는 것으로 고려되었다. The main objective of the present study is to observe the effect of a carcinogen, 3'-methyl-4-dimethylaminoazobenzene(3'-MeDAB), on bile duct epithelial cells in Clonorchis-infected mice. The animals used were 90 male BALB/c albino mice, and divided into 3 groups: group I, treated with 0.03% 3'-MeDAB(Kwasei Ind. Co., Seoul, Korea) alone for 12 weeks; group II, given approximately 200 Clonorchis metacercriae alone, and group III, given 200 metacercariae at the end of the 12 week treatment with 0.03% 3'-MeDAB. From the 16th week when the autopsies have commenced, 3 mice were removed at random from each group and examined grossly and histopathologically at 4 week interval up to the 52nd week from the beginning of the experiment. For histopathological examination, liver tissues were stained with hematoxylin-eosin. The results obtained are summarized as follows; 1. The gross findings and histopathological changes observed were remarkable in groups II and III, particularly in group III. Mild to severe mucinous cell metaplasia and goblet cell metaplasia were demonstrable in epithelial cells of the adenomatous hyperplastic glands and the biliary ductules peripheral to the second-order bile ducts. Whereas, in group I there were no significant tissue changes except the lower degrees of bile duct proliferation. cholangiofibrosis and inflammatory cell infiltration which were transient. 2. Mice in groups I and II did not develop tumors at any stage. On the other hand, there were strong histopathological evidences of neoplastic transformation of epithelial cells of the biliary ductules and hyperplastic glandular tissues in 2 out of 30 mice in Group III throughout the observation period. The cells were identified as those of transitional stages in the development of cholangiocarcinoma. With the overall findings it was clear that a carcinogen, 3'-MeDAB, played a potentiating role as a promotor for the turnover of metaplastic biliary epithelial cells induced due to Clonorchis infection into neoplastic cells in mice

어린이들의 요충 검사는 왜 필요한가

민홍기,Min, Hong-Gi 한국건강관리협회 1986 건강소식 Vol.10 No.4

신선,무해 해야 할 야채에 붙어있는 기생충

민홍기,Min, Hong-Gi 한국건강관리협회 1986 건강소식 Vol.10 No.10

인체기생충에 의한 인체의 피해

민홍기,Min, Hong-Gi 한국건강관리협회 1986 건강소식 Vol.10 No.6

봄철, 기생충을 몰아냅시다

민홍기,Min, Hong-Gi 한국건강관리협회 1988 건강소식 Vol.12 No.3

어떤 구충제든 1회 복용으로 완전 구충을 기대하는 것은 무리이며, 여러 종류의 기생충이 혼합 감염되어 있을 때 이들 모두를 한꺼번에 몰아낸다는 것은 곤란하다. 따라서 1차복용 후 재검사로 구충효과를 평가하는 것이 현명하다. 기생충증은 많은 경우 감염되었다는 사실을 모르고 지내기도 하며, 알면서도 대수롭지 않게 여겨 버리기도 하나 선진국 대열에 진입하는 나라의 국민으로서 그리고 올림픽경기를 개최하는 나라의 국민으로서 금년 1988년의 봄철에는 모두가 구충에 대한 계획을 세우고 적극적으로 참여하였으면 싶은 심정 간절하다.

개蛔蟲 幼蟲 感染 마우스에 있어서의 白血球의 變動

閔弘基 梨花女子大學校 醫科大學 醫科學硏究所 1991 EMJ (Ewha medical journal) Vol.14 No.4

A study on white blood cells(WBC) in BALB/c mice infected with Toxocara canis larvae was carried out. Mice were divided into 4 groups ; group Ⅰ and group Ⅱ was given a single dose of 30 and 100 infective eggs, respectively,and group Ⅲ and group Ⅳ was given 100 eggs weekly for 2 weeks and 50 egg for 4 weeks from I week after an inital administration of 30 eggs. respectively. Total WBC and differential count of WBC were examined at 2 week intervals up to the 20th week. An immediate leucocytosis took place; slight in group I, mild in group Ⅱ, moderate in group Ⅲ and marked in group Ⅳ. In differential count, neutrophils in group Ⅰ and group Ⅱ reached the lowest level in the 2nd week, then returned to within normal limits, while in group Ⅲ and gruop Ⅳ showed a rise, respectively. Eosinophils in group Ⅰ and group Ⅱ respectively reached a maximum peak in the 2nd week and in group Ⅲ and group Ⅳ respectively showed a quiker and more intense rise in the earlier stage, and decreased rapidly for the next several weeks, then fell gradually. Lymphocytes in group I decreased gradually up to the 6th week, then returned to within normal limits, but in groups Ⅱ, Ⅲ and Ⅳ showed a more or less rapid decrease in the earlier stage and persisted up to the end of observation without remarkable variation, Monocytes in groups Ⅰ and Ⅱ were within normal limits, and in groups Ⅲ and Ⅳ showed some monocytoses. No alterations were noted in the basophile count.

유구낭미충증은 어떤 기생충 병인가?

민홍기,Min, Hong-Gi 한국건강관리협회 1983 건강소식 Vol.7 No.10

시각장애자용 문자인지 장치에 관한 연구

허웅,조병서,김진곤,함광근,민홍기 明知大學校 産業技術硏究所 1994 産業技術硏究所論文集 Vol.13 No.-

In this paper, we implemented the hangeul tactile recognition system for the Blind. The system is consist of hardware part and software part.The hardware part is consist of a scanner interface image processing system, a electro tactile output device inter and auser interface. The software system consist of pattern learning routine,image processing and character pattern output routine, file management and operation window routine. The 16 by 16 of return electrode is driven by a electro-tacttile device. The recognition experimention distinguished learning beforefrom learning after,after learning the recognition rate is higher than learning before. As a results of experimentation before and after learning,the later is superior to the former. Therefore,if learning is increased, we can take higher recognition rate.

Mouse에 있어서의 犬蛔蟲 幼蟲의 胎內感染에 對한 Cortisone의 影響

閔弘基 梨花女子大學校 韓國生活科學硏究院 1974 韓國生活科學硏究院 論叢 Vol.13 No.-

犬蛔蟲의 感染期卵子로 感染된 姙娠 mouse에게 cortisone을 一定期間 投與하여 諸組織, 子宮, 胎盤 및 胎兒에의 同幼蟲의 移行樣相에 미치는 效果를 追試한 바 그 成績을 要約하면 다음과 같다. 1. 姙娠期間中 感染된 mouse에 있어서 cortison 投與群에 比하여 感受性의 增加 및 抵抗力의 減少가 나타난다고 推定되며 對照群에 比해 約 2倍의 幼蟲이 檢出되었고 胎兒에로의 移行도 훨씬 增加되었다. 2. 姙娠 2週前에 感染시킨 mouse에 있어서는 cortison의 投與與否와 關係없이 胎兒에로의 幼蟲의 移行을 볼수 없어 이는 感染時期로 보아 胎兒에게 二次的 移行은 이루어지지 않았음을 推定케 한다. 3. 胎兒에로의 移行 幼蟲의 增加는 胎盤 또는 胎兒에 對한 cortisone의 選擇的인 效果라기 보다는 宿主 自體의 多樣性인 感受性에 미친 影響에 依한 一次的 移行의 結果라고 思料된다. An experimental study on the effecacy of "Cortisone" in pregnant mice infected at specified times with 1,000 infective stage eggs of Toxocara canis was carried out. According to the experimental schedule as shown in figure 1, mice in groups Ⅰ,Ⅱ and Ⅲ were infected on the 10th day and sacrificed on the 17th day of pregnancy. On the other hand, mice in the groups Ⅱ and Ⅲ were injected with cortisone, subcutaneously, with a dose of 0.5mg. once daily for 2 weeks during pergnancy and before gestation, respectively. Mice in groups Ⅳ and Ⅴ were infected two weeks before gestation and the latter were treated with cortisone from the day of infection to the gestation. The results are summerized as follows; 1. There were highly significant differences in the number of larvae recovered from various tissues including uterus, fetus and placenta between group Ⅰ and Ⅱ or group Ⅱ and Ⅲ. Group Ⅱshowed that the recovered number of larvae which has migrated into uterus, fetus and placenta were almost twice more than those in group Ⅰ or Ⅲ. 2. Group Ⅴ showed no larva of Toxocara canis in the fetus but a few in the uterus or in the placenta on the 17th day of pregnancy. But no larva was found in the fetus, even in the uterus and placenta in group Ⅳ on the day after the same duration as that in group Ⅴ. 3. Administration of cortisone in pregnancy mice may cause increased susceptibility to infection by Toxocata canis larvae and depressed host-resistance against this parasite. From the above results, it is suggested that the larvae found from the fetus in the uterus were introduced by the primary migration soon after infection rather than by secondary migration of the larvae from other tissues in pregnant mice.

臟器幼蟲迷入症

閔弘基 대한의학협회 1976 대한의학협회지 Vol.19 No.5