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Pellet Semen 의 Glycerol 첨가방법과 평형시간이 한우의 정액성상과 수태에 미치는 영향
김종규,서국성,신원집,이용빈 ( J . K . Kim,G . S . Suh,W . J . Shin,Y . B . Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.2
This experiment was conducted to study the possibility of pellet semen as a replacement for ampoule semen and simplifying the method of glycerol addition to the egg yolk extender with Korean native cattle. Glycerol was added once and twice to the egg yolk semen extender and equilibration time was 6, 12 and 18 hrs. respectively. Each semen was freezed in the liquid nitrogen and thawed in a 5℃ water bath. Inseminated artificially frozen semen to the cows and calculated the fertility (N.R. 60-90 days) and sperm living index. The results obtained are as follows: 1. No significant difference was found between once and twice additions of glycerol on the living index of spermatozoa. 2. No significant difference was found between, 6, 12 and 18 hours equilibration intervals on the living index of spermatozoa. 3. Conception rates in the Korean Native Cattle of pellet and ampoule semen were 55.1% and 59.2% respectively. The results indicated that conception rate of pellet semen was no better than the ampoule semen.
김종규 한국콩연구회 1991 韓國콩硏究會誌 Vol.8 No.1
간장 醱酵의 主要 酵母인 S. rouxii와 T. versatilis 두 균주의 성질을 함께 가진 새로운 菌의 開發을 目的으로 異屬 酵母間의 原形質體 融合을 實施하였다. 이렇게 하여 얻은 融合體를 香氣生成用 培地에서 培養한 후, 그 生成 香氣의 芳香性 檢査와 香氣成分을 分析한 gas chromatogram을 野生菌株와 比較하여 얻은 優良 融合體 ST 726-F20, ST 723-F30, 그리고 ST 723-F31은 우수한 간장 香氣를 生成하였고, HEMF와 4-EG를 생성하였으며, 22∼25%의 높은 監濃度에서도 生育하여 監에 대한 耐性이 강하였고 넓은 範園의 pH에서도 生育이 可能하여 産業的(一般 醱酵난 bireactor)으로 利用하기에 좋은 菌으로 사료되었다.