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        답 다년생 잡초방제에 관한 연구 1 : 추경이 다년생잡초의 방제에 미치는 영향

        金吉雄,崔鉉玉 韓國作物學會 1976 Korean journal of crop science Vol.21 No.1

        다년생 잡초의 지하번식기관의 토양중 분포조사와 추경심도 및 AVIROSAN에 의한 다년생 잡초 가래 방제에 미치는 영향에 대하여 얻어진 예비결과를 요약하면 다음과 같다. 1. 너도방동산이와 올미의 괴경은 주로 0∼5cm의 깊이에 얕게 분포하여 있으나 가래의 인경과 올방개의 괴경은 각각 87%, 65%가 주로 10cm 이상의 깊이에 분포하였다. 2. 추경 20cm는 무경운, 무제초에 비하여 약 63%나 가래의 발생을 적게 하였다. 3. AVIROSAN 10a당 3kg 처리는 가래 방제에 특효하였으며 수량도 손제초 2회에 비하여 4% 증수되었다. 4. 보리짚 10a 당 250kg 처리도 가래의 발생억제는 물론 손제초 2회보다 1% 증수한 결과를 보였다. Distribution of underground organs of perennial weeds in soil and ecological control measure were evaluated. Tubers of Cypers serotinus and Sagittaria pygmaea were mostly presented in a shallow depth of 0 to 5cm, however, Potamogeton distinctus's bulbs and Eleocharis kuroguwai's tubers were distributed in the soil at 10 to 20cm. A 20cm autumn plowing was, to some extent, effective to reduce Potamogeton distinctus. The herbicide, avirosan at 3kg/10a alone, was extremely effective in controlling Potamogeton distinctus.

      • 殺蟲劑에 의한 染色體異常誘發에 관한 硏究

        金吉雄 順天鄕大學校 1974 의대논문집 Vol.1 No.-

        Studies on the chromosomal aberrations of plant carried out on root of onions with various concentrations(ppm) of four insecticides. Four insecticides including two organic phosphorus insecticides (Diazinone and DDVP) anb two organochloric insecticides (Thiodane and Chlorinated camphene) were examined. Onions were soaked in freshly prepared solutions of one of the insecticides at the various concentration of 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, ppm for 7days. Root tips were fixed in carnoy's fixative solution(Ethanol-Glacial acetic acid-chloroform, 6:1:3) and stained by Feulgen method. The highest frequency of chromosomal aberrations in oniens was chlorinated camphene. Chlorinated camphene was injected into the peritoneal cavity of rats as following concentrations, 100, 200, 300, 400, 500, 600, 700, 800, ppm with 2㎖ of each every day for 7 days. The chromosomal aberrations were examined by Giemsa stain after the peripheral blood culture had been treated with TC-199 and colcemide. 1. The mean percentage of chromosomal aberrations was represented 4,263% with Diazione 5,534% with DDVP 8,502% with Thiodan and 10,804% with chlorinated camphene. 2. The more the toxicity of insecticides was strengthened, the more the frequency of chromosomal aberrations during the metaphase increased. 3. The more the difference of concentrations of insecticides was increased, the more the frequency of chromosomal aberrations increased. 4. The phases of chromosomal aberrations were shown as the bridges and fragments. 5. The results of t-test were represented t=10, 772>3.25=p0.01 with Diazinone, t=7,842>3.25=p0.01 with DDVP, t=15,630>3.25=p0.01 with Thiodane, t=19,123>3.25=p0.01 with Chlorinated camphene. The following results were obtained by animals. 6. The results of t-test were shown as t=4,695>3.71=p0.01 with Chlorinated camphene. 7. The mean percentage of chromosomal aberrations was represented 10,066% with Chlorinated camphene. 8. The significant difference of chromosomal aberrations with animals was less than with plants by the difference of concentrations of chlorinated camphene. 9. The phases of chromosomal aberrations were shown as the fragments, sticky and sticky-fragments.

      • 雜草防除試驗 遂行方法 및 評價法 : 圃場試驗을 中心으로 Emphasis on the Field Trials

        金吉雄 慶北大學校 새마을硏究所 1979 農村과 科學 Vol.2 No.-

        This paper attempted to outline the evaluation methods conducting weed control research. It is essential for well designed, carefully prepared and accurately handled experiments to obtain the meaningful results. The purformance of a herbicide can be greatly affected by a number of factors, particularly environmental factors such as rainfall, light intensity, soil texture, % organic matter, and soil moisture etc. After establishment of weed control trials and the treatments applied it is important to precisely and regularly assess the effects of the treatments on the crops and the weeds. The methods of weed assessment like visual weed ratings, weed number counts, weed height and weed dry weight, and crop assessment such as visual estimation, stand counts, stand height, crop appearence, yield and crop quality were described and discussed. Interpretation of results should be based on economic analysis, considering all factors affecting the purformance of a herbicide.

      • 培養 筋細胞의 分化에 따른 分泌蛋白質에 관한 硏究

        김길웅,유병제 대구대학교 기초과학연구소 1984 基礎科學硏究 Vol.1 No.-

        Several proteins were detected in the muscle-conditioned medium strongly suggesting that the cells in cultured released polypeptides which might act on the membrane of neighboring cells to initiate the fusion. The muscle-conditioned mediums of fusion arrest conditions were analyzed. It was showned that the muscle-conditioned medium promotes the muscle cell fusion.

      • 목화자엽에 함유된 Glycosidase들의 생리학적 역할에 관한 연구 : 목화자엽에 함유된 α-Mannosidase의 특성

        김길웅,이갑숙,이조광 대구대학교 산업기술연구소 1987 産業技術硏究 Vol.6 No.-

        목화자엽의 glycosidase들 가운데 α-mannosidase의 특성과 생리적 기능에 대하여 연구하였던 바, 효소의 최적 pH는 3.8과 4.2로 나타났으며 K_(m)값과 V_(max)값은 각 각 3.70mM과 0.66 ㎛/mg/min 이었다. 또한 α-mannosidase는 기질에 대한 특이성을 가지고 있어 p-nitrophenyl-α-D-mannopyranoside, Xanthan, Soybean lectin 등을 가수분해 시켰다. Cotton α-mannosidase의 특성은 Jack bean meal의 것과 매우 비슷하여 Ovalbumin과 Soybean lectin과 같은 glycoprotein으로부터 mannose 를 분리시킬 수 있었다.

      • Clofibrate(CPIB)로 飼育한 家兎의 血淸 脂質濃度에 관한 硏究

        金吉雄,趙萬熙 조선대학교 기초과학연구소 1979 自然科學硏究 Vol.2 No.1

        This study was undertaken to determine the effect of serum lipid in rabbits fed with clofibrate diet. The five group of rabbits were fed with clofibrate, which analyzed free cholesterol easter, triglyceride and phospholipid for ten fays. The results were as follows : 1. There was no large difference between the serum lipid levels in gthe group fed with clofibrate 0.1㎎/day and control group in rabbits. 2. The serum lipid levels in the group fed with clofibrate 0.2㎎/day was as a result of free cholesterol 17.0±2.1 cholesterol ester 37.8±4.0 phosphlipid 47.2±3.3, triglyceride 73.1±3.6㎎/100㎖ in rabbits. 3. The serum lipid levels in the groups fed with clofibrate 0.3㎎/day was a result of free cholesterol 15.4±2.4, cholesterol ester 36.8±3.3, phospholipid 39.4±3.4, triglyceride 67.7±3.5㎎/100㎖ in rabbits. 4. The serum lipid levels in the groups fed with clofibrate 0.4㎎/day was as result of free cholesterol 14.2±2.3, cholesterol ester 33.6±4.8 Phospholipid 32.3±2.8, triglyceride 58.9±3.5㎎/100㎖ in rabbits. 5. From the above results we obtained the Normal serum lipid levels in rabbits could be confirmed by free cholesterol 20.0±2.3, cholesterol ester 41.9±4.8, phosphlipid 53.7±3.6 and triglyceride 78.4±2.6㎎/100㎖ in rabbits.

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