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Dong Hoon Cho(조동훈),Mi Young Chung(정미영),Sun Ok Jee(지선옥),Chang Kil Kim(김창길),Jae Dong Chung(정재동),Kyung Min Kim(김경민) 한국생명과학회 2009 생명과학회지 Vol.19 No.9
본 연구는 제주도에서 자생하는 새우난초 3개체, 금새우난초 3개체 그리고 변이종 14개체를 포함하여 총 20개체를 화색에 따라 분류하고 유전자지도를 작성하여 QTL분석을 하였다. 화색은 새우난초가 어두운 자색으로 CIE Lab값이 40~50 정도였으며, 금새우난초는 황색으로 110~130 정도였고, 변이종 개체들은 새우난초와 유사하거나 다소 높았다. PCR 결과 얻은 polymorphism이 인정되는 154개 marker에 대한 분리비 적합도 검정에서 51개 marker에서 5% 수준의 유의성이 인정되었으며, 유의성이 인정된 51개 marker 중에서 새우난초 type은 37개, 금새우난초 type은 14개 였다. Polymorphism이 인정된 154개 marker에 대하여 MAPL program을 이용하여 이들 marker 상호간의 연관관계를 분석한 결과는 16개의 연관군과 1개의 독립군으로 구분되었으며, 이들 연관군에 대한 분자연관지도는 전체 group의 크기가 220.4 cM (centi Morgan)이고, marker 간의 평균거리는 3.3 cM이었다. 양적 형질에 대한 분자연관지도상의 QTL 분석 결과, LOD 3.0 이상인 화색 과 설판색의 QTL은 각각 3개와 1개였다. 이상에서 얻어진 자료는 새우난초 속의 화색 연구에 도움이 될 것으로 사료된다. This study was conducted to clarify the genetic relationship between Calanthe discolor, C. sieboldii and variants, and the cause of flower color variations by using a molecular linkage map and a quantitative trait loci (QTL) analysis for flower and lip color in Calanthe species native to Korea. Twenty plants were included in three C. discolor and three C. sieboldii, and fourteen variants were obtained from their habitat, Jeju-do in Korea. The flowers of C. discolor were brownish red, the values of Commission Internationale de I'Eclairage (CIE) Lab were between 40 and 50. The flowers of C. sieboldii were yellowish, the values of CIE Lab were between 110 and 130. The variants had various mixed colors that were thought to have originated from natural hybridization between C. discolor and C. sieboldii, and the values of CIE Lab were between 50 and 70. The colors of the lips were usually divided into white and yellow. C. discolor had a white lip, C. sieboldii had a yellow one, and the variants had a white to yellow one. The CIE Lab value of each color was 90 in white and 110 to 120 in yellow lips. A molecular linkage mapping was constructed based on the segregation of 154 RAPD markers using a MAPL program. Sixteen linkage groups containing 66 markers were established. It covered a total map distance of 220.4 cM. The distance between adjacent markers ranged from 0 to 6.6 cM, with an average distance of 3.3 cM. These markers are thought to be closely associated with flower and lip color expression. Among the 16 molecular linkage groups, 3 QTLs had flower color trait loci and 1 QTL had lip color trait loci.
Molecular Dynamic Simulation of Melting/Solidification Rate of Ar
정재동(Jae Dong Chung) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Molecular Dynamics (MD) simulations have been conducted to look at the melting and solidification of Lennard-Jones argon (100) interface at small amounts of undercooling and superheating, 6.0K. By combining the fully equilibrated bulk phases of liquid and solid in one simulation box and counting the number of solid - like particle, the interface velocity, i.e. growth rate or melting rate, as a function of temperature was obtained. The melting temperature where no growth or melting of crystal particle is expected T<SUB>m</SUB><SUP>*</SUP> =0.668which is close to that of Gibbs free energy calculation. Linear slope dependency on temperature was found except for high superheating, △ T>6K. Thus the high superheating is believed as the main source of slope discontinuity in the rate, not the misuse of initial regime.
정재동(Jae Dong Chung) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Molecular Dynamic Simulation has been conducted for the melting process of Ar and the effect of defect on the melting temperature was examined. Drastic change of volume and energy is expected to be involved with phase change. In pure crystal, hysteresis is found due to the superheating and supercooling, but a defect decreases the superheating. Asymptotic behavior is found as the strength of defects increases the temperature where the volume changes drastically approaches to the experimental value of melting temperature. Thus the melting point can be expected by observing the change of drastic volume change.