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Determination of Sucrose Content in Soybean Using Near-infrared Reflectance Spectroscopy
Choung, Myoung-Gun The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.4
This study was to investigate near-infrared reflectance spectroscopy (NIRS) application for determination of individual soluble carbohydrate content in soybean seeds for development of high-quality new soybean variety and quality control of soy products. NIRS was used as a rapid and simultaneous analysis method to determine individual soluble carbohydrate content of soybean seeds in Korea. A total of 240 samples (about 2 g) were scanned in the reflectance mode and the reference values for the individual soluble carbohydrate contents were measured by high performance liquid chromatography. Calibration equation for individual soluble carbohydrate contents were developed using modified partial least-squares regression with internal cross validation (n=155). The equation for sucrose content had lower standard error of cross-validation, higher $R^2$ (coefficient of determination in calibration), and higher ratio of unexplained variance divided by variance (1-VR) value than that for raffinose, and stachyose. Prediction of an external validation set (n=85) showed significant correlation between reference value and NIRS estimated value based on the standard error of prediction (SEP), $r^2$ (coefficient of determination in prediction), and the ratio of standard deviation (SD) of reference data to SEP. The model for sucrose had higher value of RSP (ratio of standard deviation for prediction, the ratio of SD of reference data to SEP(C) in the external validation set) and $r^2$ (3.50 and 0.921, respectively), thereby that equation has good quantitative accuracy, whereas those of raffinose, and stachyose had lower value (below 0.89 and 0.45, respectively). These results indicated that NIRS could be used to rapidly determine the content of sucrose in soybean seed samples in the breeding programs for development of high-quality new soybean variety.
Myoung Gun Choung 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.4
This study was to investigate near-infrared reflectance spectroscopy (NIRS) application for determination of individual soluble carbohydrate content in soybean seeds for development of high-quality new soybean variety and quality control of soy products. N
( Myoung Gun Choung ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.4
This study was to investigate near-infrared reflectance spectroscopy (NIRS) application for determination of individual soluble carbohydrate content in soybean seeds for development of high-quality new soybean variety and quality control of soy products. NIRS was used as a rapid and simultaneous analysis method to determine individual soluble carbohydrate content of soybean seeds in Korea. A total of 240 samples (about 2 g) were scanned in the reflectance mode and the reference values for the individual soluble carbohydrate contents were measured by high performance liquid chromatography. Calibration equation for individual soluble carbohydrate contents were developed using modified partial least-squares regression with internal cross validation (n=155). The equation for sucrose content had lower standard error of cross-validation, higher R2 (coefficient of determination in calibration), and higher ratio of unexplained variance divided by variance (1-VR) value than that for raffinose, and stachyose. Prediction of an external validation set (n=85) showed significant correlation between reference value and NIRS estimated value based on the standard error of prediction (SEP), r2 (coefficient of determination in prediction), and the ratio of standard deviation (SD) of reference data to SEP. The model for sucrose had higher value of RSP (ratio of standard deviation for prediction, the ratio of SD of reference data to SEP(C) in the external validation set) and r2 (3.50 and 0.921, respectively), thereby that equation has good quantitative accuracy, whereas those of raffinose, and stachyose had lower value (below 0.89 and 0.45, respectively). These results indicated that NIRS could be used to rapidly determine the content of sucrose in soybean seed samples in the breeding programs for development of high-quality new soybean variety.
Myoung Gun Choung,Hannah Lee,Si Young Lee,Su Jin Yang,Seon Kang Choi,Chang Yeon Yu,Jung Dae Lim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Lutein, a xanthophyll, consists of chains with 8 conjugated double bounds containing closed rings on each end of the chain. This carotenoid is found in fruits and vegetables, especially dark green leafy vegetables such as green tea. In this study, we investigated the anticancer effects of purified lutein from green tea on human cancer cell lines containing prostate carcinoma cancer cells (LNCaP). Methods and Results : Prostate carcinoma cancer cells (LNCaP) were cultured and evaluated the inhibitory effect of lutein isolated from green tea compared other carotenoids (β-carotene and lycopene) on cell proliferation. Cyclin D1 and PCNA were evaluated as cell differentiation. In results, PCNA/cyclin regulates the initiation of cell proliferation by mediating DNA polymerase. Under cultural conditions, lycopene remarkably suppressed the PCNA expression prostate cancer cell line LNCaP in higher doses (20 μM - 100 μM) statistically. However, β-carotene and lutein presented the less inhibitory effects on PCNA expression. Determination of PCNA expression in control and treated cells demonstrates that lycopene did affect proliferation in LNCaP cancer cells in dose-dependent manner. However, β-carotene and lutein suppressed the cyclin D1 expression in dose-dependent manner but no in lycopene group. These results indicate that differ carotenoids presented the various suppressive ability of PCNA and cyclin D1 expression in cell proliferation. Conclusion : In conclusion, lutein suppressed the carcinogenesis of induced prostate cancer cell line by acting as a suppressor for inhibiting the expression of cyclin D.
ESR 및 In Vitro Assay를 이용한 녹차 아세톤 추출물과 이로부터 분리된 루테인의 항산화 활성
Myoung Gun Choung,Si Young Lee,Hannah Lee,Su Jin Yang,Seon Kang Choi,Chang Yeon Yu,Jung Dae Lim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Plants are the rich source of antioxidants, which plays a very important role in maintaining human health. Their antioxidant property protects cells of different organs of human beings against free radicals and free radical mediated diseases. Even though, there is lack of knowledge on the antioxidant effect of lutein present in plants. In the present study, lutein was isolated from the GreenTea leaves (Camellia sinensis) which is used as a dietary source. Methods and Results : The procedure adopted for the isolation and purification of lutein using acetone extraction and preparative high performance liquid chromatography (HPLC) is simple and less time consuming. Free radicals scavenging activity of isolated lutein from acetone extract of GreenTea was assessed by DPPH radical scavenging assay and reducing power. The isolated lutein scavenged 79% of DPPH radicals at 20 ㎍/㎖ and two fold lower concentration compared to the standard antioxidants (α-tocopherol). No significant differences were found between the reducing power of the lutein and BHT when their concentrations were high. However, significant differences were observed at relatively low concentrations, the reducing power of lutein was isolated from the GreenTea leaves was stronger than those of their acetone extract and standard antioxidants (BHA). Both electron spin resonance (ESR) and in vitro assay confirmed that lutein was isolated from the GreenTea leaves, exhibited a greater capacity for scavenging superoxide (O2 •-) and hydroxyl (OH •) radicals than standard antioxidants β-carotene and α-tocopherol respectively. Conclusion : The results proven that lutein isolated from GreenTea leaves has an efficient antioxidant ability, it could serve as an antioxidant to scavenge reactive oxygen species.
Myoung Gun Choung,Si Young Lee,Hannah Lee,Eun Ji Go,Chang Yeon Yu,Jung Dae Lim 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05
Backgrounds : Camellia sinensis is known to have a very high antioxidant activity, but its petals are small and it is difficult to use it because of its low yield. In comparison Camellia japonica has many petals and yield, however, the biological effects of C. japonica have been less frequently studied. In the present study, we focused on investigating the in vitro antioxidant effect of the ethanol extract from flower of C. sinensis (CSF), C. japonica (CJF) and mixture of CSF and CJF. Methods and Results : Content of total phenolics and total flavonid, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities, reducing power, superoxide anion and hydroxy radical scavenging activity of CSF and CJF were compared in vitro experimetal models. Total phenolics was contained the higer in CJF (172.19±1.65 mgCAE/gEX) than 146.75±0.15 mgCAE/gEX in CSF. And effects of antioxidant measured by reducing power, superoxide anion generated by xanthine/xanthine oxidase and hydroxyl radical generated by the Fenton reaction (FeSO4 + H2O2) in a cell-free system was shown higher in mixture of CSF and CJF than BHT, ascorbic acid as a chemical oxidant which was detected by electron spin resonance spectrometry coupled with DMPO spin trapping. These results suggest that Camellia flower extract such as CSF and CJF exhibits antioxidant properties by scavenging ROS. Camellia extract contained quercetin, quercetin-3-O-glucoside, quercitrin and kaempferol, which are antioxidant compounds. Conclusion : As a result, the combination of CSF and CJF showed higher antioxidative effect than using CSF or CJF alone.
Isolation and Determination of Phenolic Compounds in Peony (Paeonia lactiflora Pall.)Root
Choung, Myoung-Gun,Kang, Kwang-Hee,An, Young-Nam The Korean Society of Crop Science 2000 한국작물학회지 Vol.45 No.2
The five phenolic compounds of peony root were isolated by Sephadex LH-20 column chromatography. Their chemical structures were identified by spectroscopic methods (UV, FT-IR, FAB-MS and $^1H$ .&^13C$-NMR). The complete structures of these compounds were elucidated to be (+)-taxifolin-3-0-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin. The concentrations of five phenolic compounds in the peony root of three Korean cultivars (Youngchonjakyak, Euisungjakyak and Jomjakyak) were determined by reverse-phase HPLC. The constituents concentration in Youngchonjakyak were generally higher than in Euisungjakyak and Jomjakyak. The concentrations of (+)-taxifolin-3-O-$\beta$-D-glucopyranoside, benzoic acid, gallic acid, (-)-epicatechin and (+)-catechin in three different cultivars were ranged 0.23-0.52%, 0.20-0.30%, 0.26-0.28%, 0.09-0.12% and 0.34-0.63%, respectively.