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Lee, Eujin,Kim, Sun-Gun,Park, Na-Young,Park, Hyo-Hyun,Jeong, Kyu-Tae,Choi, Jongkeun,Lee, In-Hae,Lee, Hwadong,Lee, Eunkyung Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.50
<P><B>Background:</B></P><P>A Korean herbal medicine, KOTMIN13, composed of <I>Inula japonica</I> Thunberg, <I>Trichosanthes kirilowii Maximowicz</I> var. <I>japonica kitamura</I>, <I>Peucedanum praeruptorum</I> Dunn, and <I>Allium macrostemon</I> Bge, has been used for anti-allergic and anti-asthmatic treatment in oriental clinics, but its activity has not been investigated.</P><P><B>Materials and Methods:</B></P><P>To evaluate the anti-inflammatory activity of KOTMIN13 for <I>in vitro</I> study, LPS-stimulated RAW 264.7 cells were used to induce the production and expression of inflammatory mediators and its mechanisms. 12-<I>O</I>-Tetradecanoylphorobol-13 aceate (TPA)-induced ear edema and carrageenan-induced paw edema models were also used to evaluate the effect of KOTMIN13 on acute inflammation <I>in vivo</I>.</P><P><B>Results:</B></P><P>KOTMIN13 reduced the release of inflammatory mediators [nitric oxide, prostaglandin E2, interleukin (IL)-1β, and IL-6] and the protein expression of inducible nitric oxide synthase and cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Mechanism studies showed the attenuation of LPS-induced NF-κB activation by KOTMIN13 via IκBα degradation abrogation and a subsequent decrease in nuclear p65 levels. Activation of mitogen-activated protein kinases (ERK, JNK, and p38) was also suppressed. Furthermore, KOTMIN13 ameliorated the development of TPA-induced ear edema and carrageenan-induced paw edema in acute inflammatory edema mouse models.</P><P><B>Conclusion:</B></P><P>Our study demonstrates that KOTMIN13 inhibits inflammatory mediators through the inhibitions of NF-κB and MAPK activities in LPS-induced RAW 264.7 cells, as well as acute inflammation in edema models, indicating that KOTMIN13 is an effective suppressor for anti-inflammatory activities.</P><P><B>SUMMARY</B></P><P><P>KOTMIN13 decrease the production of No, PGE<SUB>2</SUB>, and proinflammatory cytokine (TNF-∝, IL-1β,IL-6).</P><P>KOTMIN13 Suppressed the degradation of NF-kβ and IKβα and the phosorylation of MAP Kinases.</P><P>Topical application of KOTMIN13 reduced mouse ear edema.</P><P>Oral administration of KOTMIN13 decreased carrageenan-induced paw edema.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> NO: nitric oxide; PGE2: prostaglandin E2; iNOS: inducible NO synthase; COX-2: cyclooxygenase-2; TNF-α: tumor necrosis factor-α; IL: interleukin; NF-κB: nuclear factor kappaB; MAPK: mitogen-activated protein kinases; ERK: extracellular signal regulated kinase; JNK: c-jun N terminal kinase; TPA: 12-O-tetradecanoylphorbol-13-acetate</P>
이은경 ( Eunkyung Lee ),서민지 ( Minji Seo ),이경상 ( Kyeong-sang Lee ),최성원 ( Sungwon Choi ),이다래 ( Darae Lee ),진동현 ( Donghyun Jin ),권채영 ( Chaeyoung Kwon ),김홍희 ( Honghee Kim ),허모랑 ( Morang Huh ),한경수 ( Kyung-soo 대한원격탐사학회 2017 大韓遠隔探査學會誌 Vol.33 No.3
본 연구에서는 산출 방법에 따른 해빙 자료 간의 차이를 조사하기 위하여 1980-2010년 북극 지역의 EUMETSAT OSI SAF, NASA Team(NT)의 해빙 농도와 해빙 면적의 차이를 비교하였다. 그 결과 두 자료의 해빙 농도와 해빙 면적이 계절별, 해역별로 다른 일관성을 보였다. 계절별로는 OSI SAF의 해빙 농도가 전체적으로 0.85 %, 봄 0.48 %, 여름 0.97 %, 가을 1.38 %, 겨울 0.66 % 높게 나타났다. 해역별로는 북극해에서 OSI SAF의 해빙 농도가 2.7 %, 해빙 면적이 19.8만 ㎢ 높았으나 링컨해 일부에서는 해빙 농도가 2.3 %, 해빙 면적이 2만 ㎢ 낮게 나타났다. We compared sea ice concentration(SIC) and sea ice extent(SIE) using EUMETSAT Ocean and Sea Ice Satellite Application Facilities(OSI SAF) and NASA Team(NT) sea ice algorithm in the Arctic during 1980-2010 to investigate the difference between sea ice data applied different algorithms. SIC and SIE of the two data showed different consistency by season and by sea area. Seasonally, SIC of OSI SAF was 0.85 % overall, 0.48 % in spring, 0.97 % in summer, 1.38 % in autumn and 0.66 % in winter higher than NT SIC. By sea area, OSI SAF SIC was 2.7 %, SIE was 198,000 ㎢ higher than NT in Arctic Ocean, but in Lincoln Sea, OSI SAF SIC was 2.3 %, SIE was 20,000 ㎢ lower than NT.
Lee, Jiyoul,Chung, Jong Won,Jang, Jaeman,Kim, Do Hwan,Park, Jeong-Il,Lee, Eunkyung,Lee, Bang-Lin,Kim, Joo-Young,Jung, Ji Young,Park, Joon Seok,Koo, Bonwon,Jin, Yong Wan,Kim, Dae Hwan American Chemical Society 2013 Chemistry of materials Vol.25 No.9
<P>The influence of alkyl side chains on the crystallinity of semiconducting copolymer films and their sub-bandgap density-of-states (DOS), the latter being closely related to the stability and the device performance of organic field-effect transistors (OFETs), is investigated. Three different poly(hexathiophene-alt-bithiazole) (PHTBTz) based polymer semiconductors, with identical backbones but different side chain positions and lengths, were synthesized. The crystallinity examined by grazing incidence X-ray diffraction (GIXRD) strongly depends on the number, position, and length of each type of alkyl side chain attached to the thiophene and thiazole copolymer backbones. Also, the sub-bandgap trap DOS distributions were extracted by performing multiple-frequency capacitance–voltage (MF-CV) spectroscopy on the field effect devices. The relationship between film crystallinity and trap DOS in the field-effect transistors can be interpreted in terms of the complex interplay between the number, position, and length of each alkyl side chain for efficient π–π stacking. In particular, the number and position of the alkyl side chain attached to the polymer backbone significantly affects the device performance. Poly(tetryloctylhexathiophene-alt-dioctylbithiazole) (PHTBTz-C8) exhibits the best electrical performance among the different semiconductors synthesized, with a relatively low bulk trap density of ∼2.0 × 10<SUP>20</SUP> cm<SUP>–3</SUP> eV<SUP>–1</SUP> as well as reasonable hole mobility of ∼0.25 cm<SUP>2</SUP> V<SUP>–1</SUP> s<SUP>–1</SUP>. The microstructural analyses of this organic material strongly suggest that the short π–π stacking distance induces strong interaction between adjacent polymer backbones, which in turn results in enhanced electrical properties.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2013/cmatex.2013.25.issue-9/cm400592b/production/images/medium/cm-2013-00592b_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm400592b'>ACS Electronic Supporting Info</A></P>
Lee, Eunkyung,Choi, So-Young,Yang, Jae-Ho,Lee, Youn Ju The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.4
Early life neuronal exposure to environmental toxicants has been suggested to be an important etiology of neurodegenerative disease development. Perfluorohexanesulfonate (PFHxS), one of the major perfluoroalkyl compounds, is widely distributed environmental contaminants. We have reported that PFHxS induces neuronal apoptosis via ERK-mediated pathway. Imperatorin is a furanocoumarin found in various edible plants and has a wide range of pharmacological effects including neuroprotection. In this study, the effects of imperatorin on PFHxS-induced neuronal apoptosis and the underlying mechanisms are examined using cerebellar granule cells (CGC). CGC were isolated from seven-day old rats and were grown in culture for seven days. Caspase-3 activity and TUNEL staining were used to determine neuronal apoptosis. PFHxS-induced apoptosis of CGC was significantly reduced by imperatorin and PD98059, an ERK pathway inhibitor. PFHxS induced a persistent increase in intracellular calcium, which was significantly blocked by imperatorin, NMDA receptor antagonist, MK801 and the L-type voltage-dependent calcium channel blockers, diltiazem and nifedipine. The activation of caspase-3 by PFHxS was also inhibited by MK801, diltiazem and nifedipine. PFHxS-increased ERK activation was inhibited by imperatorin, MK801, diltiazem and nifedipine. Taken together, imperatorin protects CGC against PFHxS-induced apoptosis via inhibition of NMDA receptor/intracellular calcium-mediated ERK pathway.
Formation of ZnO nanostructures grown on Si and SiO2 substrates.
Lee, Seungjin,Park, Eunkyung,Lee, Jongtack,Park, Taehee,Lee, Sang-Hwa,Kim, Jae-Yong,Yi, Whikun American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.9
<P>ZnO nanorods are grown on Si-based substrate by chemical bath deposition method in aqueous solution using zinc nitrate hexahydrate. Various substrates having different surface morphology are used to evaluate their effect on growing ZnO nanorods, such as flat Si(100) wafer, small and large textured-Si wafer, porous silicon, flat SiO2 wafer, small and large textured-SiO2 wafer. The length, diameter, geometry, and coverage density of ZnO nanorods are investigated by field-emission scanning electron microscopy and summarized. SiO2 is a preferred substrate for the growth of ZnO nanorods to Si if the surface morphology of substrate is same, and the textured surface has much higher coverage density (> 95%) than the flat surface. Each nanorod is vertically grown along the c-axis on the top of each pyramid face for textured substrate, and forms the 3D sea sponge-like ZnO structure. The characteristics of ZnO nanorods grown on various substrates are analyzed by grazing-angle X-ray diffraction (XRD) and photoluminescence (PL) measurements.</P>
Lee, Eunju,Lee, Bo Bin,Ko, Eunkyung,Kim, Yujin,Han, Jungho,Shim, Young Mog,Park, Joobae,Kim, Duk‐,Hwan Wiley Subscription Services, Inc., A Wiley Company 2013 Cancer Vol.119 No.9
<P><B>Abstract</B></P><P><B>BACKGROUND:</B></P><P>The objective of this study was to discover molecular biomarkers associated with the recurrence of esophageal squamous cell carcinoma (ESCC).</P><P><B>METHODS:</B></P><P>The authors retrospectively analyzed the hypermethylation status of 11 genes using methylation‐specific polymerase chain reaction (PCR) and the expression of epidermal growth factor receptor (EGFR), O‐6 methylguanine‐DNA methyltransferase (MGMT), tumor protein 53 (p53), and transforming growth factor β (TGFβ) using immunohistochemistry in 329 formalin‐fixed, paraffin‐embedded ESCCs.</P><P><B>RESULTS:</B></P><P>Recurrence was identified in 151 of 329 ESCCs (46%) at a median follow‐up of 4.5 years. The recurrence was associated with hypermethylation of the genes cell adhesion molecule 1 (<I>CADM1</I>) (<I>P</I> = .003), deleted in colon carcinoma (<I>DCC</I>) (<I>P</I> = .04), or cyclin‐dependent kinase inhibitor 2A (<I>p14</I>) (<I>P</I> = .02) in patients with stage I ESCC. Thirty‐six of 37 Stage I ESCCs (97%) that had cohypermethylation of at least 2 of the 3 genes had hypermethylation of <I>p14</I> plus either <I>CADM1</I> or <I>DCC</I> or both <I>CADM1</I> and <I>DCC</I>. The 5‐year recurrence‐free survival (RFS) rates were 93% in patients who had stage I disease without hypermethylation of the 3 genes and 56% in those who had cohypermethylation of <I>p14</I> in combination with <I>CADM1</I> and/or <I>DCC</I>. Patients who had stage I ESCC with cohypermethylation of <I>p14</I> in combination with <I>DCC</I> and/or <I>CADM1</I> had 7.13 times (95% confidence interval, 1.61‐31.64 times; <I>P</I> = .009) poorer RFS compared with those who had no hypermethylation of the 3 genes after adjusting confounding factors. Hypermethylation of the other 8 genes and altered expression of 4 proteins were not associated with recurrence across pathologic stages.</P><P><B>CONCLUSIONS:</B></P><P>The current results suggested that cohypermethylation of <I>p14</I> in combination with <I>DCC</I> and/or <I>CADM1</I> may be an independent prognostic factor for recurrence in patients with stage I ESCC. Cancer 2013. © 2013 American Cancer Society.</P>