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- 저자 : ( Daimon Syukri ) (검색결과 2 건)
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Change of Phospholipid Species in Japanese Mustard Spinach Leaves during Senescence
( Daichi Nagaya ),( Daimon Syukri ),( Manasikan Thammawong ),( Kohei Nakano ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
In this study, a wide target analysis of phospholipid species in Japanese mustard spinach leaves stored for different durations was conducted by mass spectrometry, and phospholipid class distribution and fatty acid composition were compared for the purpose of identifying the freshness maker metabolites. Self-cultivated Japanese mustard spinach (Brassica rapa var. perviridis) leaves were harvested and stored for 4 and 7days at 20°C. Lipid in the leaves was extracted by a MTBE method and brought to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The multiple reaction monitoring (MRM) program for a total 141 phospholipids was prepared and used for a highly sensitive detection. Peak area of MRM chromatogram for each phospholipid molecule were calculated and compared using t-statistics. The highest number of phospholipids (43) were detected on day 7 whereas 41 was found on day 0. Almost all the phosphatidylcolines (PC) and phosphatidylinositols (PI) increased during senescence, but the other phospholipid classes were found unchanged. Especially, PI (14:1/16:1) and PC (32:1) increased significantly and were suggested to be a potential freshness marker metabolite. Further study is needed to confirm these findings through the quantitative determination.
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Andriyana Setyawati,Samanhudi Samanhudi,Wuri Prameswari,Daimon Syukri,Defrita Fitri Ramadhani,Okky Talitha 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.1
Euchresta horsfieldii (Lesch) Benn. is a highly demanded medicinal plant with many benefits. In vitro propagation through callus induction is an effective method for rapid multiplication in a short time. This research aimed to evaluate the effective concentration of Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic acid on callus induction and organogenesis for in vitro propagation and secondary metabolite production of E. horsfieldii. The design used in this research was a Complete Randomized Design in 2 factors: Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic acid with 5 levels for each concentration. Parameters observed are the percentage of inducted calluses, callus appearance time, the weight of fresh calluses, the weight of dry calluses, the texture of callus, the color of callus, percentage of the formed shoot, shoot appearance time, the height of shoots, the number of leaves, flavonoid compounds of callus, and bioactive compounds. The result showed that the single treatment of Benzyl Amino Purine and 2,4-Dichlorophenoxiacetic was significantly affecting the shoots forming. The concentration of 2,4-Dichlorophenoxiacetic 0 ppm gave the highest average plantlet height by 2.67 cm, increasing the number of shoots by 2.00, and the number of leaves by 5.60. E. horsfieldii cultured in vitro without additional growth regulators had a higher flavonoid content.
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