의이인추출물이 DSS(dextran sulfate sodium)로 유발된 생쥐의 궤양성 대장염에 미치는 영향 = (The) effects of Coicis semen extract(CSE) on dextran sulfate sodium-induced colitis in mice

다국어 초록 (Multilingual Abstract)

Objective: This study was carried out to investigate the effects of Coicis Semen Extract (CSE) on the experimental colitis induced by dextran sulfate sodium(DSS) in mice.

Method: Experimental colitis was induced in mice by daily treatment with 5% DSS in the drinking water for 7days in 6-week-old male ICR mice. The colitic mice were divided into three groups: the normal(N) group consisted of mice that were not inflammation-induced. The control(C) group was composed of untreated colitis elicited mice. The sample(S) group was administered CSE after colitis elicitation. The effects on colonic mucosal ulcers were evaluated by the morphological, histological and immunohistochemical change of the large intestine (colon, cecum and rectum).

Results: Inhibition of LPS-induced NO was decreased in S group. In RAW264.7 cell, NO inhibitory effect of S group at 2.0mg/ml was counted to be 14.8%. Inhibition of LPS-induced iNOS and COX-2 mRNA was noticeably decreased in S group from 0.25mg/ml. In the common morphological and histochemical change, C group showed the erosion and the infiltration of inflammatory cells are increased in C group, while they are noticeably decreased in S group. The length of colon was shortened in C group than in S group. The distributions of MUC2 and Hsp70 in S group treated with CSE noticeably increased in S group than in C group (p<0.05). It is confirmed histochemically and immunohistochemically that the distributions of iNOS, COX-2, MAC387, serotonin, apoptosis and PCNA treated with CSE decreased in S group than in C group (p<0.05).

Conclusions: According to the above results, it is concluded that Coicis Semen Extract (CSE) has a potential protective effect on colitis.

목차 (Table of Contents)

• I. 서론 = 1
• II. 실 험 = 2
• 1. 재료 = 2
• 1) 실험동물 = 2
• 2) 세포주와 세포배양 = 2
• 3) 약재 = 2
• 4) 대장염 유발 및 실험동물군 = 3
• 5) 세포에 CSE 첨가량 = 3
• 2. 방법 = 4
• 1) NO 생성 측정 = 4
• 2) 염증효소 유전자 발현 측정 = 4
• 3) 조직표본준비 = 4
• 4) 조직화학 및 면역조직화학적 염색 = 5
• 5) 세포자연사 = 6
• 6) 영상분석 및 통계처리 = 6
• III. 결과 = 7
• 1. RAW264.7 세포를 이용한 염증억제 효과 = 7
• 1) RAW264.7 세포에서의 NO 생성억제 효과 = 7
• 2) RAW264.7 세포에서의 염증효소 유전자 발현억제 효과 = 8
• 2. 동물실험을 이용한 CSE의 효과 = 9
• 1) 체중 변화 = 9
• 2) 대장의 길이 변화 = 10
• 3) 대장의 형태적 변화 = 11
• 4) CSE의 세포보호 효과 = 11
• 5) CSE의 염증억제 효과 = 16
• 6) 신경전달물질 Serotonin의 발현 = 19
• 7) 세포자연사 = 19
• 8) 세포재생 효과 = 22
• IV. 고 찰 = 23
• V. 결론 .29
• 참고문헌 = 30
• ABSTRACT = 34