The efficiency of Deer antler Herbal Acupuncture on modulation and prevention of IL-1 mediated activation in rat chondrocytes at a receptor level = 쥐 연골모세포에서 IL-1으로 매개된 활성을 수용체 수준에서 조절 및 억제하는 녹용약침(DHA)의 효과

국문 초록 (Abstract)

항염작용이 있는 녹용이 관절염의 중간 발병 매개체 IL-1β가 세포 수용기에 결합하는 것을 억제할 수 있는지 연구했다. IL-1β나 ROS로 처리된 쥐의 연골세포에 녹용을 첨가하면 proteoglycan(PG)의 합성을 억제한다. 그러나 ROS 자극후에는 PG합성을 억제하지 않는다. IL-1β로 유도된 상태에서 녹용은 Ⅱ형 IL-1β수용체를 암호화하는 mRNA의 합성과정을 증가시켰다. 이러한 결과는 IL-1β가 신호하는 경로의 두 가지 조정 단백질의 잠재적 역할을 강조하면서 골관절염에 있어서 녹용의 효과를 설명할 수 있다.
본 연구는 녹용이 매개한 항염활성의 새로운 기전을 증명하였다. 정상 인체 관절연골모세포에서 IL-1β로 유도된 NO생산과 TNF-α로 유도된 NO생산 모두를 녹용이 억제한다는 것을 보여주었다. IL-1으로 유도된 NO생산의 억제는 NO합성 효소 mRNA와 유전자 단백질 합성과정의 억제와 연관되어 있다. 녹용은 또한 IL-1으로 유도된 IL-6의 합성을 억제한다. 결론적으로 이러한 결과는 녹용이 독특한 범주의 활성을 합성하며 염증과정을 방해하는 새로운 기전을 증명한다.

다국어 초록 (Multilingual Abstract)

Deer antler Herbal-Acupunture (DHA) solution represents one of the most commonly used medicine to treat osteRArthritis. But, mechanisms of its antiarthritic activities are still poorly understood. Identification of common DHA aqua-acupunture capable of affording protection or modulating the onset and severity of arthritis may have important human health implications. DHA has been shown to possess anti-inflammatory and anticarcinogenic properties in experimental animals. We determined if DHA could prevent the binding of IL-1β to its cellular receptors. DHA addition to rat chondrocytes treated with IL-1β or with reactive oxygen species(ROS) prevents the activation of proteoglycan synthesis. After treatment with IL-1β, DHA increased the expression of mRNA encoding the type II IL-1β receptor. These results emphasize the potential role of two regulating proteins of the IL-1β signaling pathway that could account for the beneficial effect of DHA in osteRArthritis. The present study also identifies a novel mechanism of DHA-mediated anti-inflammatory activity. It is shown that DHA inhibits both IL-1β- and TNF-α-induced NO production in normal human articular chondrocytes. The observed suppression of IL-1-induced NO production is associated with inhibition of inducible NO synthase(iNOS) mRNA and protein expression. In addition, DHA also suppresses the production of IL-1-induced cyclooxygenase-2 and IL-6. The constitutively expressed cyclooxygenase-1, however, was not affected by the sugar. In conclusion, these results demonstrate that DHA expresses a unique range of activities and identifies a novel mechanism for the inhibition of inflammatory processes.

목차 (Table of Contents)

• Contents
• Abstract = 4
• Ⅰ. Introduction = 5
• Ⅱ. Materials and methods = 6
• 1. Materials = 6
• 2. Methods = 7
• 1) Chemicals = 7
• 2) Rat chondrocytes cultures and treatment = 7
• 3) Measurement of PG biosynthesis = 9
• 4) Analysis of IL-1RⅡmRNA expression by quantitative RT-PCR = 9
• 5) Quantification of nitrites = 10
• 6) IL-6 Measurement = 10
• 7) Statistical Analysis = 10
• Ⅲ. Results = 11
• 1. Only IL-1β induced changes on PG synthesis = 11
• 2. IL-1β induced an increase in the expression of IL-1RⅡ mRNA = 13
• 3. Inhibitory of IL-1-induced NO production = 15
• 4. IL-1-induced IL-6 production = 17
• Ⅳ. Discussion = 19
• References = 25
• Korean abstract = 28