Pure IgG was isolated from the cord sera by DEAE-C column chromatograph using 0.02 M sodium phosphate buffer, pH 8.0. The procedure has been proved to be a relatively simple method for separation of gamma globulin from serum, and applied many times wi...
Pure IgG was isolated from the cord sera by DEAE-C column chromatograph using 0.02 M sodium phosphate buffer, pH 8.0. The procedure has been proved to be a relatively simple method for separation of gamma globulin from serum, and applied many times with the recovery rate of about 70%.The purity of the IgG obtained was proved by the microimmunoelectrophoretic technic and animal immunization followed by double gel diffusion test.
A comprehensive comment on the analytic methods of the serum protein and concept of the immunoglobulins was also described.ㄴ