肝疾患者의 血淸中 Adenosine 3',5' Cyclic Monophosphate와 Guanosine 3',5' Cyclic Monophosphate의 level에 關한 硏究 = Studies on cyclic AMP and cyclic GMP level in human serum with liver disease

다국어 초록 (Multilingual Abstract)

Isocratic separation of cyclic AMP and cyclic GMP level in serum is established by means of H.P.L.C.
This method Is applicated for the measurement of cyclic AMP and cyclic GMP level in human serum with liver disease patients (hepatitis: 10, liver cirrhosis: 10, hepatoma:10) and the normal (11).
The obtained results are summerized as follows:
1. In the normal serum, cyclic GMP/cyclic AMP ratios have approximately 1:2
2. Cyclic AMP and cyclic GMP level in hepatitis are equal to and/or tend to reduce compairing with the normal.
3. In serum of patients with liver cirrhosis and hepatoma, cyclic AMP level are similar to the normal level and/or tend to decrease.
4. In serum of patients with liver cirrhosis and hepatoma, cyclic AMP level is increased remarkedly, and the tendency of cyclic GMP/cylic AMP ratios show hepatoma > liver cirrhosis > the normal (>＝) hepatitis.
5. In serum of patients with liver cirrhosis and hepatoma, cyclic GMP Level is elevated.
The reason is not clear, although possible contributory factors may be compensatory proliferative changes in liver, decreased hepatic uptake of cyclic nucleotide metabolism.
6. Appearently increasing cyclic GMP level in the patients with hepatoma suggests that it is the useful tool on diagnosis of hepatoma.

목차 (Table of Contents)

• 目次 = ⅰ
• Ⅰ. 序論 = 1
• Ⅱ. 實驗方法 = 8
• 1. 試藥 및 試液 = 8
• 2. 裝置 = 8
• 3. 實驗材料 = 8
• 4. 定量法 = 9
• 가. 標準溶液의 調製 = 9
• 나. 試料溶液의 調製 = 9
• 다. 實驗法 = 9
• Ⅲ. 結果 및 考察 = 11
• 1. 測定條件의 檢討 = 11
• 가. 吸收 波長의 選擇 = 11
• 나. 移動相의 選擇 = 11
• 다. 流速의 影響 = 12
• 라. 檢量線 = 12
• 2. 正常人 및 肝疾患者의 血淸中 cyclic AMP및 cyclic GMP의 濃度測定 = 14
• Ⅳ. 結論 = 20
• 參考文獻 = 21
• ABSTRACT = 25