Preparation and characterization of water-insoluble carboxymethyl cellulose foam by acid treatment

다국어 초록 (Multilingual Abstract)

A sodium carboxymethyl cellulose (Na-CMC) foam was obtained by lyophilization of aqueous Na-CMC solution and chemically treated with citric acid in order to control the water-solubility and liquid handling properties. The objective of this study was to prepare the water-insoluble CMC (H-CMC) foams and to investigate the liquid handling properties and their applicability to wound management materials. The chemical structure was analyzed by FT-IR, ICP, TGA, and XRD. The structural analyses revealed that the substitution of sodium atom in Na-CMC to hydrogen atom during the acid treatment resulted in more hydrogen bonding in the molecular structure. Acid concentration and treatment time influenced LAR and LARP of the foam. The increase in the amount of acid and treatment time decreased the liquid absorption capacities of the CMC foams. Nevertheless, all the H-CMC foams showed excellent liquid absorption, more than 30 g/g. The expansion ratio and the liquid handling properties of compressed foam were affected by the compression temperature. In case that the temperature was above 80℃, the expansion ratio and the liquid absorption capacity decreased. The biocompatibility evaluation was performed by cytotoxicity test. In the cytotoxicity test, the result showed that the cell viability of extracted solution, diluted to 50%, showed 90% contrast to the control. This approach to controlling the shape stability and liquid handling properties by acid treatment of Na-CMC foam is expected to find useful applications in the development of novel moisture wound dressing materials.

목차 (Table of Contents)

• 1. Introduction 1
• 2. Theoretical backgrounds 3
• 2-1. Wounds 3
• 2-2. Wound exudate 6
• 2-3. Wound dressings 6
• 2-3-1. Introduction of wound dressings 6
• 2-3-2. Types of wound dressing . 7
• 2-4. Properties of ideal foam dressings . 11
• 2-5. Weakness of typical wound dressings 11
• 2-6. Carboxymethyl cellulose . 12
• 3. Materials and Methods 14
• 3-1. Materials 14
• 3-2. Preparation of Na-CMC foam . 14
• 3-3. Acid treatment of Na-CMC foam 14
• 3-4. Liquid handling properties 15
• 3-4-1. Liquid absorption ratio (LAR) . 15
• 3-4-2. Liquid absorption ratio under pressure (LARP) . 16
• 3-5. Statistical analysis . 16
• 3-6. Cytotoxicity . 20
• 3-6-1. Cell line and Cell Culture . 20
• 3-6-2. Sample Preparation 20
• 3-6-3. Cell Viability Test 20
• 3-7. Expansion after liquid absorption 21
• 3-8. Other characterizations . 22
• 4. Results and discussion 23
• 4-1. Selection of acid 23
• 4-2. Preparation of H-CMC foam 32
• 4-3. Morphological Analyses of CMC foam 32
• 4-3-1. Macroscopic observation . 33
• 4-3-2. scanning electron microscopy 34
• 4-4. Structural Analyses of CMC foam 36
• 4-4-1. FT-IR 36
• 4-4-2. ICP 36
• 4-4-3. Thermogravimetric analysis 37
• 4-4-4. XRD . 40
• 4-5. Liquid handling properties 41
• 4-6. Expansion after liquid absorption . 44
• 4-7. Cytotoxicity test 50
• 5. Conclusion . 53
• REFERENCES
• ABSTRACT(KOREAN)