강황추출물이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 간암과 간 손상에 미치는 영향

다국어 초록 (Multilingual Abstract)

In order to investigate the effect of CLR extract on the Hepatocellular carcinogenesis and acute liver damage induced by DENA and CCl4 in Rats. Experimental groups were subdivided into four; normal group (Nor), acute liver damage and hepatocellular cancer inducing control group (Con), and CLR extract 200㎎/㎏/day (CAA) or 400㎎/㎏/day (CAB) administered groups to Con.
Thereafter the changes of the body weight, the liver weight and the weight of liver/100g body weight, Total cholesterol, HDL cholesterol, Triglyceride, the activities of AST, ALT, ALP, LDH, AFP, SOD, Catalase were measured. And we observed by optical and electron microscopy.
The results were as follows,
1. The body weight was decreased in Con compared with Nor for 5 weeks, but increased in Con compared with Nor from 6 week to 9 week. During experimental period of total 9 weeks, CAA and CAB were increased compared with Con.
2. The liver weight was increased significantly (p<0.05) in Con compared with Nor. The weight of liver/100g body weight was increased significantly (p<0.05) in Con compared with Nor and decreased significantly (p<0.05) in CAB compared with Con.
3. The level of Total cholesterol was increased in Con and CAA compared with Nor, but there was not statistically significant. The level of Triglyceride was decreased in Con compared with Nor. But increased in CAA and CAB compared with Con. The level of HDL-cholesterol was significantly increased (p<0.05) in CAA and CAB compared with Con.
4. The activities of AST, ALT were increased in Con compared with Nor, but decreased in CAA compared with Con, significantly decreased (p<0.05) in CAB compared with Con.
5. The activities of ALP, LDH were increased in Con compared with Nor, but decreased in CAA and CAB compared with Con.
6. The activities of AFP was increased significantly (p<0.05) in Con compared with Nor, but decreased significantly (p<0.05) in CAA and CAB compared with Con.
7. The activities of SOD were increased in Con, CAA and CAB compared with Nor, but decreased in CAA and CAB compared with Con. The activities of Catalase was more increased in CAA and CAB compared than Con.
8. The results of light microscopical observation, a number of hepatocytes were damaged in Con compared with Nor and CAB.
9. According to the electron microscopical observation, irregular nuclear membrane, condensed nucleoplasm was observed in Con, the experimental group was observed in the nucleus of the well-preserved and evenly developed nucleoplasm.

These results suggest that administration of CLR extract suppress or retard on the Hepatocellular carcinogenesis and acute liver damage induced by DENA and CCl4 in rats.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 재료 및 방법 3
• 1. 재료 3
• 1) 실험 동물 3
• 2) 약재 및 약물 추출 3
• 2. 방법 3
• 1) 간 손상 및 간암 유발 4
• 2) 실험군 설정 및 약물 투여 4
• 3. 관찰방법 4
• 1) 체중 측정 4
• 2) 간 중량 측정 4
• 3) Total cholesterol, HDL cholesterol, Triglyceride 측정 5
• 4) 혈청 Transaminase 활성 측정 5
• 5) Alkaline phosphatase(ALP) 활성 측정 5
• 6) Lactate dehydrogenase(LDH) 활성 측정 5
• 7) 혈청 AFP 측정 6
• 8) Superoxide Dismutase(SOD) 활성도 측정 6
• 9) Catalase 활성도 측정 6
• 10) 광학현미경 관찰 7
• 11) 전자현미경 관찰 7
• 12) 통계처리 8
• Ⅲ. 결 과 9
• 1) 체중의 변화 9
• 2) 간 중량 및 체중 100g 당 간 중량의 변화 10
• 3) 혈청 지질 함량의 변화 12
• 4) 혈청 Transaminase 활성의 변화 15
• 5) Alkaline phosphatase(ALP) 활성의 변화 17
• 6) Lactate dehydrogenase(LDH) 활성의 변화 18
• 7) 혈청 Alpha fetoprotein(AFP) 활성의 변화 19
• 8) SOD와 Catalase의 활성의 변화 20
• 9) 간조직의 변화 22
• 10) 간세포의 미세구조변화 24
• Ⅳ. 고 찰 30
• Ⅴ. 결 론 36
• 참 고 문 헌 38