베타3-아드레날린성 수용체 유전자 다형에 따른 혈중 기질 및 안정시 대사량의 비교 = Comparison of obesity indices, blood substrates, and resting metabolic rate according to β3-adrenergic receptor gene polymorphism

다국어 초록 (Multilingual Abstract)

The purpose of the current study was to compare anthropometrics, obesity indices, cardiorespiratory fitness, and obesity-related between non-obese and obese groups across the β3-adrenergic receptor gene polymorphism. Apparently healthy Subjects (non-obese = 53, obese=37, aged 20-30 year old) were recruited via flyers and local advertisements on a campus news letter. Following an overnight fasting, the subjects visited to our laboratory three times on separate days. On the first visit, anthropometrics and body composition were measured. Venous blood samples were taken for genomic DNA extraction to determine the β3-adrenergic receptor genotypes by using polymerase chain reaction. On the second visit, 20-ml of fasting venous blood samples was collected from an antecubital vein into EDTA-treated collection tubes for blood lipids (TC, HDL-C, FFA, TG, and LDLC), glucose, analyses. Minute maximum oxygen consumption and maximum heart rate were measured on a motor-driven treadmill by using the Bruce protocol. On the final visit, resting metabolic rate was determined via an indirect calorimetry by using a metabolic cart.
The results were as follows;
1. The relative frequencies of the b3-AR genotypes were not significantly different between the obese and non-obese groups.
2. Within the obese group, the TA heterozygotes had significantly higher values in body weight, fat free mass, BMI, %BF, waist circumference, and waist-to-hip ratio than the TT homozygotes, while no such differences were found in the non-obese group.
3. There was a significant group by genotype interaction in mean RMR values such that in the obese group, the TA heterozygotes had a higher value than the TT homozygotes, while no significant genotype difference was found in the non-obse group.
4. There were significant group differences in blood pressure and lipoprotein-lipids between the obese and non-obese groups such that the former had higher SBP, DBP, MAP, TC, and LDL-C values, but a lower HDL-C values than the latter.
The current findings of the study suggest that the β3-AR gene polymorphism can magnify a genetic predisposition to obesity and obesity-related metabolic syndrome.

목차 (Table of Contents)

• 목차
• Ⅰ. 서론 = 1
• 1. 연구의 필요성 = 1
• 2. 연구의 목적 = 7
• 3. 연구의 가설 = 7
• 4. 연구의 제한점 = 7
• 5. 용어 정리 = 8
• Ⅱ. 이론적 배경 = 10
• 1. 비만의 정의 = 10
• 2. 에너지 균형과 체중의 조절 = 11
• 1) Set-Point 가설 = 11
• 2) 에너지 균형과 체중 조절의 고전 이론들 = 12
• 3. 안정시 대사량 = 14
• 1) 안정시 대사량의 변화에 미치는 요인 = 17
• 4. 아드레날린 수용체 = 18
• 1) β3-adrenergic 수용체의 특징 = 18
• 2) 아드레날린 수용체와 지방분해 = 20
• 3) β3-adrenergic 수용체 유전자의 변이 = 22
• Ⅲ. 연구방법 = 24
• 1. 연구대상 = 24
• 2. 실험설계 및 절차 = 24
• 3. 실험내용 및 방법 = 25
• 1) 신체계측 = 25
• 2) 안정시 대사량 및 최대산소섭취량 측정 = 25
• 3) 혈액채취 = 25
• 4) 측정항목 및 분석방법 = 26
• 4. 자료 처리 방법 = 32
• Ⅳ. 연구결과 = 33
• 1. β3-adrenergic수용체 유전자의 상대적 형질 빈도 분석 = 33
• 2. 체격지수와 비만지표 비교 = 35
• 3. 심폐체력, 안정시 대사량 비교 = 40
• 4. 혈압 및 혈액변이 비교 = 44
• Ⅳ. 논의 = 47
• Ⅴ. 결론 = 51
• 참고문헌 = 52
• ABSTRACT = 64