항생제와 혈소판 유래 성장인자의 동시 전달을 위한 생체재료 플랫폼의 개발 및 특성 분석 = Development and Characterization of a Biomaterial Platform for the Co-Delivery of Antibiotics and Platelet-Derived Growth Factors

목차 (Table of Contents)

• I. Introduction 1
• II. Materials and Methods 8
• 2.1. Fabrication of Pristine and PDMS Blend Films 8
• 2.1.1. Preparation of Platelet Concentrate 8
• 2.1.2. PDMS Films 9
• 2.1.3. Preliminary Screening of Antibiotic Concentration 11
• 2.1.4. Sampling of PDMS Films 11
• 2.2. SEM Characterization of PDMS Films 12
• 2.3. Antimicrobial Activity 13
• 2.4. ELISA 14
• 2.4.1. Sample Preparation 14
• 2.4.2. Preparation of Control 15
• 2.4.3. ELISA Test Kits 16
• 2.5. Biocompatibility of PDMS Films with Mammalian Cells 17
• 2.5.1. Cell Culture and Culture Conditions 17
• 2.5.2. Seeding of HeLa Cells on PDMS Films 17
• 2.5.3. Assessment of HeLa Cell Viability Using Calcein-AM 18
• 2.6. Statistical Analysis 19
• III. Results 20
• 3.1. Preliminary Screening of Antibiotics 20
• 3.2. Characterization of PDMS Films 24
• 3.2.1. Top-Surface Characterization via SEM 24
• 3.2.2. Cross-Section Characterization of PDMS Films 27
• 3.2.3. Quantitative Analysis of Pores Found in the Cross-Section of PDMS Films 30
• 3.3. Kirby-Bauer Disk Diffusion Test 33
• 3.4. ELISA 37
• 3.4.1. Matrix-Positive Controls 37
• 3.4.2. Platelet Concentrate Controls 39
• 3.4.3. Raw Optical Density Readings and Estimated Concentrations of Growth Factor 41
• 3.4.3.1. Platelet-Derived Growth Factor-AB 41
• 3.4.3.2. Transforming Growth Factor-β1 45
• 3.4.3.3. Vascular Endothelial Growth Factor 49
• 3.5. Biocompatibility Test 52
• IV. Discussion 56
• V. References 70
• VI. 국문 초록 80