선혼합된 바이오세라믹 치수 복조재의 생물학적 효과 및 삼차원적 in vitro 실험 모델의 개발 = Biologic effects of a premixed hydraulic bioceramic pulp capping material and development of three-dimensional in vitro model

다국어 초록 (Multilingual Abstract)

Objectives
Premixed hydraulic calcium silicate cements (hCSCs) and fast-setting hCSCs were developed for manipulation convenience. The aim of this study was to analyze the effect of a premixed hCSC on the in vitro biocompatibility and bioactivity as well as its capacity to induce reparative dentin after pulp capping in an in vivo rat model. Furthermore, it was aimed to present in vitro three-dimensional (3D) experimental model which is similar to clinical situations.

Materials and Methods
In vitro, human dental pulp cells (hDPCs) were exposed to the supernatant of the tested hCSCs including EndocemMTA Premixed (Maruchi), ProRoot MTA white (Dentsply Tulsa) and Biodentine (Septodont). To evaluate biocompatibility of the tested materials, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) and cell migration assay were performed. Cell morphology attached on the surfaces of the materials was observed using scanning electron microscopy (SEM). Cytocompatibility and cell differentiation analysis was performed by immunocytochemistry using a 3D culture model.
The dental pulp of Wistar rat molar was exposed and capped with the materials. After four weeks, the rats were sacrificed and the volume of newly formed hard tissue was measured three-dimensionally using micro-computed tomography. The degree of pulpal inflammation and dentin bridge formation was evaluated by histologic analysis. Immunohistochemical staining for dentin sialophosphoprotein (DSPP) was also performed.

Results
Overall, all tested materials did not show any significant differences regarding cytocompatibility and cell differentiation in vitro. The volumes of the reparative dentin of all experimental groups were higher than that of the control group (p < 0.05). The Biodentine group showed higher value compared to the EndocemMTA Premixed group (p < 0.05). However, there was no significant difference between the EndocemMTA Premixed and ProRoot MTA white (p > 0.05). In contrast, in histological analyses, there was no differences between the tested groups regarding the dentin bridge formation and degree of pulpal inflammation (p > 0.05). Immunoreactivity for DSPP was detected within and around the pulp tissue near the newly formed dentin only in the experimental groups.

Conclusion
Within the limits of the present study, EndocemMTA Premixed has the potential to be used as a favorable pulp capping material. Furthermore, 3D cell culture pulp capping model used in this study might provide an alternative tool for cytotoxicity and bioactivity screening of pulp capping agents.

목차 (Table of Contents)

• I. 서론 1
• II. 재료 및 방법 3
• III. 결과 9
• IV. 고찰 11
• V. 결론 16
• 표와 그림 17
• 참고문헌 28