농축산 폐기물 처리를 위한 저온내성 (低溫耐性) 메탄 생성균의 특성에 관한 연구 - Ⅱ. 저온내성 (低溫耐性) Clostridia 의 분리 - = Originals : Study on Low Temperature Tolerant Methane - Producing Bacteria for the Treatment of Agricultural and Livestock Wastes

다국어 초록 (Multilingual Abstract)

This study was conducted to investigate the biochemical properties of isolated bacteria, low temperature tolerant methane-producing clostridia which were selected for using them as inoculum to anaerobic fermentation of agricultural and livestock wastes at low temperature. The results were; 1. Low temperature tolerant methane-producing clostridia were isolated from the samples which showed the high methanogenesis rate by enrichment culture at low temperature in cellulose medium. These clostridia, Clostridium botulinum SRC-64, Clostridium scatologens SRC-91 and Clostridium tyrobutyricum SRC-100, were isolated from swampy sediment at latitude 56.9。N, lake sediment IV at latitude 55.0。N, and tidal land soil II at latitude 37.0。N, respectively. The optimum growth temperature for these isolates was 37℃ and the minimum, around 10℃. They all had detectable amount of F_(420), specific coenzyme of methanogens. 2. As anaerobic fermentation products of glucose SRC-64 produced H₂, acetic, isovaleric and caproic acid, SRC-91 produced H₂, propionic, butyric, valeric, and caproic acid, and SRC-100 produced only acetic and propionic acid. The isolates were produced CH₂ ranged from 2.6 to 8.68 n moles/㎖ for 2 days at 13℃.