원산지별 그린커피빈에 대한 항산화 및 항염증 비교 연구 = A Comparative Study on Antioxidant and Anti-inflammatory Effects of Green Coffea arabica Beans by Country of Origin

다국어 초록 (Multilingual Abstract)

In this study, the extracts of green Coffea arabica bean by country of origin were tested as a potential source for natural ingredients in cosmetic products. Antioxidative activities were measured by total polyphenol, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and 2,2 -azino-bis (3- ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Anti-inflammatory activities were checked by nitric oxide (NO) assay. The total polyphenol content was found to be higher in ethanol extract than in water extracts, and in particular Ethiopia ethanol extracts had 852.39 mg/100 g, making them the highest in content. The antioxidant measurement was measured with DPPH and ABTS, indicating that antioxidant strength increased dependent on the concentration of all extracts to be superior. The cell survival rate of the extracts of green Coffea arabica beans by country of origin was confirmed by MTT assay and was close to 100 ㎍/㎖ to 100%. Anti-inflammatory activity by NO assay showed lipopolysacchride (LPS)-induced NO was significantly inhibited following treatment with extract of green Coffea arabica beans by country of origin and decreased in a concentration-dependent manner. This study confirmed the efficacy of green Coffea arabica beans by country of origin through research related to antioxidants and anti-inflammatory, and confirmed its availability as a natural material for cosmetics.

목차 (Table of Contents)

• Ⅰ. 서론 Ⅱ. 재료 및 방법 Ⅲ. 결과 및 고찰 Ⅳ. 결론 참고문헌

참고문헌 (Reference)

1 유대준, "커피인사이드" LION 75-82, 2013

2 서한석, "커피의 관능 및 감성평가 방법의 개발과 세분화된 소비자의 커피 선호 유형 분석" 서울대학교 대학원 2006

3 한지숙, "연잎, 부평초의 항산화, 항염증 및 방부 효과 연구" 한국인체미용예술학회 17 (17): 27-38, 2016

4 최선영, "식용 및 약용자원의 에스트로젠 활성과 항산화능 평가" 한국식품과학회 37 (37): 549-556, 2005

5 정진아, "식용 및 약용 양치식물에서 부위별 추출물의 항산화활성 탐색" 한국자원식물학회 20 (20): 185-192, 2007

6 진경숙, "산초 추출물 및 분획물의 항산화, 미백 및 항염증 활성" 한국미생물·생명공학회 40 (40): 371-379, 2012

7 최장숙, "밤꽃(Castanea Crenata Flos.) 메탄올 추출물의 항산화 효과" 한국식품과학회 35 (35): 1216-1220, 2003

8 김정환, "둥근마 에탄올 추출물의 항염증 효과" 한국인체미용예술학회 17 (17): 91-102, 2016

9 이정희, "국내산 식물성 식품중페놀성 물질의 함량 분석" 26 (26): 310-316, 1994

10 문영주, "고삼 에탄올과 열수추출물의 항산화, 항염증 및 항균효과" 한국인체미용예술학회 18 (18): 91-103, 2017

11 A. L. Branen, "Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene" 52 (52): 59-63, 1975

12 J. Y. Kim, "Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages" 569 (569): 321-326, 2004

13 H. Ukeda, "Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3’-1-(phenylamino)-carbonyl-3,4-tetrazolium]- bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase" 251 (251): 206-209, 1997

14 M. B. Arnao, "Some methodological problems in the determination of antioxidant activity using chromogen radicals : a practical case" 11 (11): 419-421, 2000

15 F. Nicoletta, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid] radical cation decolorization assay" 299 : 379-389, 1999

16 J. D. Dziezak, "Preservatives : Antioxidants" 40 : 94-102, 1986

17 A. Farah, "Phenolic compounds in coffee" 18 (18): 23-36, 2006

18 A. Ozer, "Oxidative Stress in Patients With Acne Vulgaris" 2005 (2005): 380-384, 2005

19 R. G. Knowles, "Nitric oxide as a signal in blood vessels" 17 (17): 399-402, 1992

20 C. Nathan, "Nitric oxide as a secretory product of mammalian cells" 6 (6): 3051-3064, 1992

21 S. W. Ryter, "Mechanisms of cell death in oxidative stress" 9 (9): 49-89, 2007

22 신지수, "Lipopolysaccharide로 유도된 RAW264.7 세포에서 MAPK에 의한 홍삼추출물의 항염증 효과" 한의병리학회 26 (26): 293-300, 2012

23 W. Grosch, "Instrumental and sensory analysis of coffee volatiles" 16 : 147-156, 1995

24 지나, "HaCaT 피부각질세포에서 들깻잎 추출물의 산화적 스트레스에 대한 항산화 효과" 한국식품영양과학회 42 (42): 161-167, 2013

25 P. Esquivel, "Functional properties of coffee and coffee by-products" 46 (46): 488-495, 2012

26 J. Carmichael, "Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing" 47 (47): 936-942, 1987

27 V. L. Singleton, "Colorimetry of total phenolics with phosphomolybdic : phosphotungstic acid reagents" 16 : 144-158, 1965

28 E. Lopez-Garcia, "Coffee consumption and coronary heart disease in men and women : A prospective cohort study" 113 (113): 2045-2053, 2006

29 M. S. Blois, "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

30 T. Lawrence, "Anti-inflammatory lipid mediators and insights into the resolution of inflammation" 2 (2): 787-795, 2002

31 L. C. Green, "Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids" 126 (126): 131-138, 1982

32 K. Andreas, "Acute Activation of Oxidative Pentose Phasphate Pathway as Fist-Line Response to Oxidative Stress in Human Skin Cells" 59 (59): 1-13, 2015