Optimization of Aerobic-anaerobic Culture Conditions for Bioplastic Raw-material Production from Euglena gracilis = 바이오 플라스틱 원료 생산을 위한 유글레나 호기 혐기성 배양조건 최적화

목차 (Table of Contents)

• CHAPTER 1: INTRODUCTION 1
• 1.1 Background 1
• 1.2 Research objectives 3
• CHAPTER 2: LITERATURE REVIEW 4
• 2.1 Bioplastic 4
• 2.1.1 BIoplastic definition and type 4
• 2.1.2 Bioplastic production from microalgae 9
• 2.2 Euglena gracilis 12
• 2.2.1 Characteristics of Euglena gracilis 12
• 2.2.2 The value-added products from E. gracilis 15
• 2.3 Growth factor affected cultivation in E. gracilis 18
• 2.3.1 Trophic mode 19
• 2.3.2 Light 20
• 2.3.3 pH 20
• 2.3.4 Temperature 21
• 2.3.5 Medium type and nutrients in the medium 21
• 2.3.6 Purging for anaerobic cultivation 22
• CHAPTER 3: MATERIALS AND METHODS 26
• 3.1 E. gracilis strains and precultivation 26
• 3.2 Experimental procedure for optimal aerobic cultivation conditions 27
• 3.2.1 pH experiment 27
• 3.2.2 Temperature experiment 27
• 3.2.3 CO2 concentration experiment 27
• 3.2.4 Initial cell density experiment 28
• 3.2.5 Medium composition experiment 28
• 3.2.6 Application of optimal aerobic culture conditions 31
• 3.3 Experimental procedure for optimal anaerobic cultivation conditions 32
• 3.3.1 Purging experiment for anaerobic cultivation 32
• 3.3.2 Anaerobic culture medium and initial pH experiment 32
• 3.3.3 Carbon source experiment 33
• 3.4 Analytical procedures 34
• 3.4.1 Biomass, cell growth, and pH 34
• 3.4.2 Paramylon 35
• 3.4.3 Sugar and succinate 36
• CHAPTER 4: RESULTS AND DISCUSSSION 37
• 4.1 Determination of optimal aerobic cultivation conditions 37
• 4.1.1 pH 37
• 4.1.2 Temperature 40
• 4.1.3 CO2 concentration 43
• 4.1.4 Initial cell density 45
• 4.1.5 Medium composition 48
• 4.1.6 Application of optimal aerobic culture conditions 52
• 4.2 Determination of optimal anaerobic cultivation conditions 54
• 4.2.1 Purging for anaerobic cultivation 54
• 4.2.2 Anaerobic culture medium and initial pH experiment 57
• 4.2.3 Additional carbon source in anaerobic cultivation 62
• CHAPTER 5: CONCLUSIONS 66
• REFERENCE 68
• 국문초록 80