Chlorinated phenolic compounds are widely used for pesticide or biocide. Especially, pentachlorophenol (PCP) among chlorinated phenolic compounds is a sort of recalcitrant biocide used primarily for wood preservation, which ubiquitous in the environme...
Chlorinated phenolic compounds are widely used for pesticide or biocide. Especially, pentachlorophenol (PCP) among chlorinated phenolic compounds is a sort of recalcitrant biocide used primarily for wood preservation, which ubiquitous in the environment, and considered a priority toxic pollutant by the U.S. Environmental Protection Agency (EPA). The PCP degrading bacteria were isolated from the herbicides treated soil and the plants` wastewater by the enrichment culture method and identified as Pseudomonas paucimobilis. When the isolate was inoculated into PAS medium containing 20㎍/㎖ of PCP and 1% glucose, the substrate (PCP) was degraded more than 99% within 26 hours. The optimum temperature and pH are 30℃ and 7.3, respectively. From the plasmid curing test it was known to that PCP degrading genes are located in bacterial plasmid and that the plasmid contained several antibiotic resistant loci in its structure. From the SDS-PAGE experiment it turned out that two bands induced by treatment of PNP were cytoplasmic and periplasmic proteins corresponding to Mw. 27,000 and 42,000, respectively.