宿主菌이 streptomycin 耐性獲得이 phage의 各種 性質에 미치는 影響 = Effect of streptomycin resistance of host bacteria on various phage properties

국문 초록 (Abstract)

著者는 Klebsiella phage-宿主菌系를 供試하면서 SM耐性菌에서 phage가 繁殖할 때 나타나는 各種現象을 調査하여 다음 結果를 얻었다.
SM耐性菌의 生菌 및 加熱菌體物質에의 phage吸着率은 正常菌의 그것들에 對한 吸着率보다 多少 더 높았다.
SM耐性菌通過 phage 및 正常 phage가 각종 SM耐性菌에서 나타내는 檢出狀況은 正常菌에서의 그것과 비슷하였다.
SM耐性菌에서의 phage繁殖은 正常菌에서보다 多少 더 不良하였으며, SM耐性菌 通過phage의 SM耐性菌內繁殖은 正常菌內繁殖 때보다 多少 더 旺盛하였다.
SM耐性度가 比較的 낮은 菌에서 phage가 나타내는 plaque 外觀은 正常菌에서의 그것과 비슷하였으나 高度의 SM耐性菌에서 나타내는 plaque 外觀은 正常菌에서의 그것과는 全然 다른 大型鮮明 plaque를 形成하였으며, SM耐性菌에서의 plaque halo 形成은 大端히 不良하였다.
SM耐性菌 및 正常균의 莢膜性多糖類는 다같이 phage 活性에 별다른 作用을 하지 못하였다.
SM耐性菌을 通過시킨 phage의 抗原性은 正常 phage의 그것과 相當히 달랐다.

다국어 초록 (Multilingual Abstract)

The author has investigated, using the Klebsiella phage-host bacteria system, the various phenomena resulted from phage multiplication in streptomycin(SM) resistant bacteria. The following results were obtained.
The phage adsorption rate to the live cell or heat extracted-bacterial substance of SM resistant bacteria was a little higher grade than that to those of normal bacteria.
The efficiency of plating of normal and SM resistant bacteria-passed phageon various SM resistant bacteria was similar to that on normal bacteria.
The phage growth in SM resistant bacteria was worse than that in normal bacteria. The growth of SM resistant bacteria-passed phage in SM resistant bacteria was a little more vigorous than that in normal bacteria.
If the SM resistance of host cell is lower grade, the appearance of phageplaque was similar to that in normal bacteria. The plaque, however, formed in the cells of higher grade-SM resistance was larger and clearer and the appearance was completely different from that formed in normal cell. And also, the plaquehalo in SM resistant bacteria was much reduced.
The capsular polysaccharides of normal and SM resistant bacteria did not show any influence to the phage activity.
The antigeniity of SM resistant bacteria-passed phage was considerably different from that of normal phage.

목차 (Table of Contents)

• 목차
• 〉Abstract〈 = 111
• 材料 및 方法 = 112
• Phage 및 宿主菌 = 112
• 培地 = 112
• Streptomycin(以下 SM) = 112
• 實驗 成績 = 112
• 供試菌의 SM抵抗力 = 112
• SM耐性 K_p에 對한 phage 吸着狀況 = 112
• K_p 5의 菌體物質로 感作한 赤血球에의 phage 吸着狀況 = 113
• SM耐性 K_p에서의 phage 檢出狀況 = 113
• SM耐性 K_p에서의 phage 繁殖狀況 = 114
• SM耐性 K_p에서 나타내는 phage의 plaque 外觀 = 115
• SM耐性 K_p의 莢膜性 多糖類가 phage 活性에 미치는 影響 = 115
• K_p 5에 6回 通過시킨 K_p phage의 抗K_p phage 血淸에 依한 中和狀況 = 116
• 抗 K_p 5 通過phage 血淸의 K_p phage 中和狀況 = 116
• 考察 = 117
• 要約 = 118
• 文獻 = 118