고지방식이로 유도된 대사이상연관 지방간질환 개선에 대한 백화사설초의 효능 연구 = Investigation of the effects of Hedyotis diffusa Willd. on metabolic dysfunction-associated steatotic liver disease (MASLD) in HFD-induced mice

다국어 초록 (Multilingual Abstract)

Investigation of the effects of Hedyotis diffusa Willd. on metabolic dysfunction-associated steatotic liver disease (MASLD) in HFD-induced mice by Bong Kyu Park Doctor of Philosophy in Science Graduate School of Kyung Hee University Advised by Prof. Bo Hyoung Jang Obesity is a global health concern closely associated with metabolic disorders such as insulin resistance, metabolic dysfunction-associated steatotic liver disease (MASLD), and cardiovascular complications. This study aimed to evaluate the anti-obesity effects of Hedyotis diffusa Willd. extract (HDE) in a high-fat diet (HFD)-induced obese mouse model. HDE administration significantly reduced body weight gain without altering food intake, suggesting its ability to regulate energy metabolism. Moreover, HDE improved glucose tolerance and insulin sensitivity, as evidenced by lower fasting glucose levels and enhanced peripheral glucose uptake. The treatment also inhibited adipocyte hypertrophy in white adipose tissue (WAT) and activated brown adipose tissue (BAT), promoting energy expenditure. HDE further demonstrated protective effects on liver health by reducing lipid accumulation and preventing the progression of MASLD. Additionally, serum lipid profiles were normalized, with HDE reducing triglycerides and LDL cholesterol while increasing HDL cholesterol levels, thereby mitigating the risk of cardiovascular diseases. These findings indicate that HDE exerts multi-faceted effects in controlling obesity and its related metabolic disorders through various pathways. However, further research is necessary to understand the precise molecular mechanisms involved and to validate these effects in human clinical trials, establishing HDE as a potential therapeutic option for obesity and MASLD management. Key words Hedyotis diffusa Willd., obesity, insulin resistance, brown adipose tissue, metabolic dysfunction-associated steatotic liver disease(MASLD).

목차 (Table of Contents)

• List of Figures ⅲ
• Abstract ⅳ
• Ⅰ. 서론 1
• II. 재료 및 방법 6
• 1. 백화사설초 시료의 준비 6
• 2. 동물 실험 6
• 3. 혈청 분석 6
• 4. 포도당 내성 검사 6
• 5. 헤마톡실린과 에오신(H&E) 염색 7
• 6. 웨스턴 블롯 분석 7
• 7. 세포 배양 7
• 8. 세포독성 분석 8
• 9. Oil Red O 염색 8
• 10. 통계 분석 8
• Ⅲ. 결과 9
• 1. 고지방식이를 섭취한 마우스의 체중 증가에 대한 백화사설초의 효과 ·· 9
• 2. 고지방식이를 섭취한 마우스에서 포도당 내성에 대한 백화사설초의 효과 11
• 3. 고지방식이를 섭취한 마우스에서 비만 연관 혈청 지수에 대한 백화사설초의
• 효과 14
• 4. 고지방식이를 섭취한 마우스의 내장지방조직 증가에 대한 백화사설초 효과 18
• 5. 고지방식이를 섭취한 마우스의 갈색지방조직에 대한 백화사설초의 효과 · 20
• 6. 고지방식이를 섭취한 마우스의 간조직에 대한 백화사설초의 효과 22
• 7. 3T3-L1 지방 세포에서 백화사설초의 지방 축적 억제 효과 24
• Ⅳ. 고찰 26
• Ⅴ. 결론 31
• 참고문헌 32