七氣湯 藥鍼液이 활성산소종, 활성질소종, 지질과산화 및 LDL의 산화에 미치는 영향 = Effect of Chilgitang Extract for Herb-Acupuncture on ROS, RNS, Lipid Peroxidation and LDL oxidation

다국어 초록 (Multilingual Abstract)

Objectives : This study was undertaken to determine the in vitro antioxidant activity of the extract for Chilgitang herb-acupuncture solution (CHAS).
Methods : The radical scavenging capacity was tested by 2,2-diphenyl- 1-picryl-hydrazyl (DPPH), hypoxanthine-xanthine oxidase system, DCFH-DA assay, nitric oxide and peroxynitrite generating system. In addition, antioxidant activity on copper and AAPH mediated human low?density lipoprotein (LDL) oxidation was measured by using TBARS assay and relative electrophoretic mobility assay. The amount of total phenolic compounds was assayed by by the Folin-Ciocalteu method.
Results : CHAS revealed a potent scavenging activity on DPPH radical (82%), superoxide anions (73%), hydroxyl radical(63%), nitric oxide (99%) and peroxynitrite (99%). Moreover, CHAS showed a strong inhibitory effect (59%) on FeCl2-ascorbic acid induced lipid peroxidation of rat liver homogenate. Moreover, CHAS markedly inhibited copper (81%) and AAPH (56%)?mediated LDL oxidation, and effectively suppressed the electrophoretic mobility during exposure of human LDL to copper ions. CHAS (82 mg/g) contained a higher concentration of total phenolic compounds than that of water extract (45 mg/g) obtained from Chilgitang.
Conclusions : These results indicate that CHAS may protect against ROS- or RNS involved diseases, including cardiovascular diseases.

목차 (Table of Contents)

• Ⅰ. 서론 = 1
• Ⅱ. 실험재료 및 방법 = 3
• 1. 약재 = 3
• 2. 시약 = 3
• 3. 藥鍼液 調製 = 4
• 4. DPPH radical 소거능 측정 = 5
• 5. Superoxide radical 소거능 측정 = 5
• 6. Hydroxylradical 소거능 측정 = 6
• 7. 지질과산화 반응 억제효과 측정 = 6
• 7.1. 간조직 균질액 조제 = 6
• 7.2. FeCl2-ascorbic acid로 유도된 지질과산화 반응 측정 = 7
• 8. Nitric oxide 소거활성 측정 = 7
• 9. Peroxynitrite 소거활성 측정 = 8
• 10. LDL의 산화에 대한 억제효과 측정 = 8
• 11. Relative Electrophoretic Mobility(REM) Assay = 9
• 12. Total Phenolic Compound 함량 측정 = 10
• 13. 통계적 처리 = 10
• Ⅲ. 실험결과 = 11
• 1. DPPH radical에 대한 소거활성 = 11
• 2. Superoxide radical 소거능 = 13
• 3. Hydroxyl radical 소거능 = 15
• 4. 지질과산화 억제효과 = 17
• 5. Nitric oxide 소거능 = 19
• 6. Peroxynitrite 소거능 = 21
• 7. LDL 산화억제 효과 = 23
• 8. REM assay를 이용한 LDL 산화억제효과 = 26
• 9. Total Phenolic Compound의 함량 = 28
• Ⅳ. 고찰 = 30
• Ⅴ. 결론 = 34
• 참고문헌 = 36
• ABSTRACT = 40