옥수수수염, 들깻잎 및 포도줄기 복합추출물의 피부면역기능 개선효과에 대한 연구 = Studies on the Synergistic Effects of Corn Silk, Perilla Leaf and Grape Stem Combinations for the Improvement of Skin Immune Functions.

다국어 초록 (Multilingual Abstract)

This study was conducted to evaluate the synergistic protective effects of mixtures of corn silk, perilla leaf and grape stem extracts (CPG mixture) in UVB-induced mouse skin damage and pruritus induced by compound 48/80. To assess this, anti-oxidant, anti-inflammation and anti-pruritus activities of CPG mixture were investigated. Results showed that CPG mixture exhibited potent 2,2′-azino-bi(3-ethylbenzthiazoline-6-sulfonicacid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and reducing power. In addition CPG mixture suppressed nitric oxide production in lipopolysaccharide-stimulated Raw 264.7 cells. CPG mixture exhibited the strongest suppressive effects of erythema and melanin index as well as skin damage compared to treatment with ascorbic acid (AA). Upon the examination of histological sections, CPG mixture significantly reduced epidermal thickness and the infiltration of mast cells in UVB-induced skin damaged mice. Treatment with CPG mixture also inhibited glutathione (GSH) depletion and lipid peroxidation in UVB-irradiated mice. Furthermore the treatment with CPG mixture inhibited compound 48/80-induced scratching behavior in mice. In conclusion, these results indicated that CPG mixture can be used as a functional food and cosmetic ingredient for treatment of UVB-related skin damage and compound 48/80-induced scratching.

Keywords: CPG mixture, anti-oxidant, anti-inflammation, scratching, erythema, melanin.

목차 (Table of Contents)

• Ⅰ. 서론 1
• Ⅱ. 재료 및 방법 4
• 1 재료 4
• 1) 시약 4
• 2) 재료 4
• 3) 세포 4
• 4) 실험동물 5
• 2 방법 6
• 1) 총 폴리페놀의 측정 6
• 2) 총 플라보노이드의 측정 6
• 3) DPPH 라디칼 소거 활성 측정 6
• 4) ABTS 라디칼 소거 활성 측정 6
• 5) 환원력 측정 7
• 6) NO assay 7
• 7) UVB 유도 손상 및 추출물의 개선효과에 대한 실험 설계 7
• 8) 피부 멜라닌과 홍반지수 측정 8
• 9) UVB 유도 피부손상에 따른 조직학적 관찰 8
• 10) UVB 유도 피부손상에 따른 비만세포 수 측정 8
• 11) 지질과산화 분석 9
• 12) 글루타치온(GSH) 정량 9
• 13) 가려움증 억제 효과 9
• 14) 통계처리 10
• Ⅲ. 결과 12
• 1 각 재료 및 복합추출물의 총 폴리페놀, 플라보노이드 함량 12
• 2 각 재료 및 복합추출물의 항산화 효과 15
• 3 복합추출물의 환원력 18
• 4 복합추출물의 NO 생성 억제 효과 20
• 5 복합추출물의 멜라닌 지수 및 홍반 지수 억제 효과 22
• 6 Hematoxylin & esoin staining 26
• 7 Toluidine blue staining 28
• 8 복합추출물 처리에 따른 MDA 함량의 변화 30
• 9 복합추출물 처리에 따른 GSH 함량 32
• 10 복합추출물의 가려움증 억제 효과 34
• Ⅳ. 고찰 36
• Ⅴ. 결론 42
• 참고문헌 43