음식 관광객을 통한 전주음식 만족도에 대한 연구 : 전주 한옥마을 중심으로

정동은 전주대학교 대학원 2016 국내석사

Selective Regulation of Micro-RNAs in Breast Cancer

손유경 전주대학교 대학원 2017 국내석사

Selective Regulation of Epithelial-Mesenchymal Transition (EMT) Using Micro-RNAs in Breast Disease

김민지 전주대학교 대학원 2017 국내석사

Most of breast cancer (BC) metastasis is caused by epithelial-mesenchymal transition (EMT) that invades other organs by changing their characteristics. A new research is required for real-time information, detection of EMT processes, and better treatment with focusing. As a small non-coding RNA molecules, microRNA (miRNA) is to degrade the target mRNA by destabilizing and suppressing the further processes of translation. Because this miRNA can bind binds through seed sites which have 2 to 8 nucleotides. Thus, one gene can be regulated by a variety of miRNAs. It is necessary to study the effect of miRNAs as new molecular mechanistic modulators in tumor progression and inflammation. In this study, I tried to find common miRNAs which are linked with the several tumor suppressor genes using big data mining and validation. I identified that miR-93-5p and miR-221-3p are associated with inflammation of breast epithelial cells. Induction of EMT in mammary epithelial cell (MCF-10A) was triggered by transforming growth factor beta (TGF-β1) treatment. In TGF-β-induced EMT processes, miR-93-5p and miR-221-3p were double checked as a common miRNAs which are elevated and linked to the degradation of five tumor suppressor genes. MiR-93-5p and miR-221-3p overexpression levels were elucidated by real-time PCR. When I verified the protein expression level of EMT markers including E-cadherin, N-cadherin, and fibronectin, the expression level of N-cadherin and fibronectin were significantly increased in EMT- induced MCF-10A. However, the level of E-cadherin was significantly reduced vice versa. Likewise, expression level of tumor suppressor gene Smad4 is significantly increased in EMT- induced MCF-10A. After that when I transfected with miR-93-5p and miR-221-3p anti-sense oligonucleotide (ASO), the reduced tumor suppressor Smad4 protein expression was recovered. Through this study, I can conclude that the data mined miRNA candidates which can regulate the five tumor suppressor genes were validation as a common miRNA in EMT-induced breast cancer progression. Keywords: microRNA, big data mining, epithelial to mesenchymal transition (EMT), tumor suppressor gene, breast cancer prevention.

옥수수수염, 들깻잎 및 포도줄기 복합추출물의 피부면역기능 개선효과에 대한 연구

황영민 전주대학교 대학원 2017 국내석사

This study was conducted to evaluate the synergistic protective effects of mixtures of corn silk, perilla leaf and grape stem extracts (CPG mixture) in UVB-induced mouse skin damage and pruritus induced by compound 48/80. To assess this, anti-oxidant, anti-inflammation and anti-pruritus activities of CPG mixture were investigated. Results showed that CPG mixture exhibited potent 2,2′-azino-bi(3-ethylbenzthiazoline-6-sulfonicacid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and reducing power. In addition CPG mixture suppressed nitric oxide production in lipopolysaccharide-stimulated Raw 264.7 cells. CPG mixture exhibited the strongest suppressive effects of erythema and melanin index as well as skin damage compared to treatment with ascorbic acid (AA). Upon the examination of histological sections, CPG mixture significantly reduced epidermal thickness and the infiltration of mast cells in UVB-induced skin damaged mice. Treatment with CPG mixture also inhibited glutathione (GSH) depletion and lipid peroxidation in UVB-irradiated mice. Furthermore the treatment with CPG mixture inhibited compound 48/80-induced scratching behavior in mice. In conclusion, these results indicated that CPG mixture can be used as a functional food and cosmetic ingredient for treatment of UVB-related skin damage and compound 48/80-induced scratching. Keywords: CPG mixture, anti-oxidant, anti-inflammation, scratching, erythema, melanin.

도시농업에 적합한 토양조건별 약용식물의 생장에 관한 연구

박정우 전주대학교 대학원 2017 국내석사

This study is to find out the best mixture of soil and medicinal plants by growth experiment of medicinal plants and soil analysis with presenting basis regarding optimal soil or medicinal plant on divers conditions in urban agriculture. Types of soil on this study are five kinds of granite soil-control(A), leaf mold and granite soil(B), earthworm soil and granite soil(C), animal vermicast soil and granite soil(D), bed soil(E), and following former study practices, the ratio of mixture is seven (these mixed soil) to three(granite soil). The six medicinal plants used in this study has been selected by recommendation from related institutions and market experts among medicinal plants which have easy accessibility in the market and high availability in urban agriculture, and proper suitability on growth as follows; Kumgangcho, Glechoma grandis, Ledebouriella seseloides, Liriope platyphylla, Dipsacus asperoides, Asparagus officinalis, and installed at total 90 experimental places for three times repeats. To identify chemical compositions in the soil before growth experiment, mixtures of earthworm soil and granite soil and bed soil which has the best option for general soil range of crop, abundant nourishment, and permeability, moisture retensien, nutrient holding capacity is regarded as having good growth of plants after soil composition analysis with pH, T-N, T-P, exchangeable cation, EC and so on. As a result, Kumgancho, Glechoma grandis, Ledebouriella seseloides, Liriope platyphylla, Dipsacus asperoides had a good growth, Asparagus officinalis had a lower growth among six medicinal plants from the mixture of earthworm soil and granite soil. Usage of the mixture of earthworm soil and granite soil was thought to be efficient in an aspect of productivity and safety, which is expected easily to use as base soil for urban agriculture, have no bad smell, and capable of earlier harvest due to good growth of plants even though there was a little difference of growth in the best mixture soil between five kinds of soils and six kinds of medicinal plants. All medicinal plants except for Ledebouriella seseloides and Liriope platyphylla had withered from the mixture of animal vermicast soil and granite soil, which was thought that salts by high EC and overuse of organic fertilizer caused over-fertilizing on plants, followed by result in withering due to the hindrance of growth. Change in the ratio of mixture from 7 to 3 to 3 to 7 of animal vermicast soil and granite soil showed a good growth of plants, which was regarded as the evidence to backup data about the hindrance of growth by over-fertilizing. Keywords : Urban agriculture, Medicinal plant, Growth characteristics, Soil condition

순창 지역 발효식품의 특성 연구 : 장류 식품을 중심으로

구본경 전주대학교 일반대학원 2018 국내석사

In order to interface with incoming food culture from around the globe and protect Korean traditional foods, especially Korean fermented foods, the subject of Korean jangryu is researched by comparing it with the study of jangryu in Sunchang, which is well known nation-wide for gochujang. Korean food culture has three characteristics. First, a bowl of rice or grains is necessary for a meal and it is complemented by side dishes. Furthermore, Korean people utilize ondol, a traditional under floor heating system that is very useful for making fermented foods because it heats the floor directly and stimulates fermentation. Also, in the past Korean people thought of food just the same as medicine, so doctors during the Joseon Dynasty prescribed some foods with gochujang for patients with abdominal pain and other symptoms. In this study, the history of Korean jangryu is categorized into the periods before and after the foundation of the Joseon Dynasty. The results show that, as found in old Chinese books, jangryu prior to the Joseon Dynasty was made and taken by the people in Goguryeo, Baekje, Silla and Goryeo Dynasty. After the Joseon Dynasty was established, jangryu was developed and flourished. Many kinds of jangryu using a variety of methods and materials have been discovered. Some of these are doenjang, gochujang, and jeubjang which are described in old Korean diaries and encyclopedia as sangayorok, jeungbosanrymgyeongje, imwongyeongjeji and joseonyoribeob. In comparison with Korean jangryu, research shows that Sunchang jangryu, especially gochujang, is deemed to be more extraordinary than the jangryu of other regions in Korea. Jangryu in Sunchang has a special oral history. Lee Sung Gye, first king of the Joseon Dynasty, was fascinated with gochujang when staying in Mt. Hoimun. Before being approved as king, he prayed to the mountain guard in Sunchang to be made king. Furthermore, several old books such as Somunsaseol, Ojuyeonmunjangjeonsango, and Gyuhapchongseo state that Sunchang gochujang is the best gochujang in the world. In the modern generation of producing jangryu in Sunchang, there are several steps in the industry progress. These include commencing, building, advancing, and settling periods. In addition, after 2004 when Sunchang was adopted as a special jangryu economiczone, it developed as a center for production in the first surging step, then the second surging step from 2010 until the present. In the last step, Sunchang was chosen to be a Korean Traditional Fermentation Cultural Industrial District. This history of Sunchang jangryu in Joseon and modern times mean that jangryu and the jangryu industry in Sunchang have evolved from the past until now, step by step. The study results found that Sunchang janryu is distinct from others. This is because of its geographical and climatic environment, the agricultural crops(red pepper, soybeans, etc. produced under contract, ingredients or resources for jangryu products as well as the people who make the products and the time and method that make authentic Sunchang jangryu.

보건복지 분야의 빅 데이터 활용 연구

김지애 전주대학교 대학원 2016 국내석사

산림경제에 수록된 약용생물자원 전통지식에 관한 연구

박진홍 전주대학교 대학원 2016 국내석사

The purpose of this study is to analyze traditional knowledge of medicinal plants, animals and fungi from the data of collected in Sallimgyeongje (Farm Management, 17-18th century) written by Hong Man-Seon (1643-1715). Quantitative analysis were accomplished using informant consensus factor (ICF) and fidelity level (FL). A total of 1,085 traditional knowledge collected from Sallimgyeongje were classified into 137 families, 237 genera, and 275 species that included plants, animals, and fungi. The representative families were Fabaceae (8.91%) followed by Liliaceae (5.51%), Zingiberaceae (4.41%), and Phasianidae (4.13%). On the whole, 62 kinds of used parts were used. Roots were most frequently used parts, constituting 25.99% of the whole followed by seeds (13.13%), leaves (6.98%), fruits (6.61%), and aerial part (4.13%). The total number of preparation methods for the medicinal materials was thirty-one: decoction (23.05%), juice (18.46%), paste (13.68%), powder (9.92%), and raw (6.80%). The informant consensus factor values in the category of diseases were poisonings (0.55), followed by others (0.46) and cut and wounds (0.4). According to fidelity levels, 118 species resulted in fidelity levels of 100%. The results suggest that medicinal resource such as plants, animals and fungi expected as new materials of medicine in the development of agriculture biotechnology. Keyword : Sallimgyeongje, traditional knowledge, ethnopharmacological resource, informant consensus factor, fidelity level

고욤나무잎 추출물의 항산화, 항염 및 피부 개선에 대한 연구

김장호 전주대학교 일반대학원 2018 국내박사

고욤나무(Diospyros lotus, DL)는 아열대 남서 아시아 및 남동부 유럽에 서식하는 고욤나무 속의 널리 재배된 종이며, 민간요법에서 여러 용도로 사용되고 있다. 이 식물의 각종 부분은 요통, 호흡곤란, 출혈, 불면증 및 딸꾹질을 완화하기 위하여 이용되어 왔고, 고욤나무잎은 전통 차 재료로도 사용되고 있다. 또한 항산화 및 항염증 특성을 포함하여 다른 생물학적 활성을 갖는 플라보노이드가 풍부한 것으로 보고되었다. 그러나 고욤나무잎의 항산화, 항염증 및 피부 보호 효과에 관한 연구는 부족한 실정이다. 따라서 본 연구의 목적은 고욤나무잎 추출물(DLE)의 항산화, 항염증 및 피부 보호 효과에 관한 생물학적 활성을 연구하고자 하였다. 이 연구의 첫 번째 장에서는 체외에서 화학 유도된 자유 라디칼 및 마우스 RBC 산화 손상에 대한 DLE의 항산화 특성을 평가하고자 하였다. DLE는 DPPH와 ABTS 유도 라디칼의 제거에 대해 높은 항산화제 활성을 보였다. DLE의 항산화제 활성은 기준 항산화제인 butylated hydroxytoluene 및 trolox와 유사 하였다. 1,000 ㎍/mL 에서 DLE의 감소력은 비타민 C와 유사했다. RBC에서 aqueous peroxyl 라디칼 생성기 AAPH에 의해 유도된 산화적 용혈은 용량 의존적으로 DLE에 의해 유의하게 억제되었다. 또한, DLE는 AAPH로 손상된 적혈구에서 세포질 항산화 글루타치온의 고갈을 방지했다. 이러한 결과는 DLE가 산화적 손상에 대해 고품질의 항산화 특성을 지닌 천연 제품으로서의 가치를 가질 수 있음을 시사한다. 이 연구의 두 번째 장은 DLE와 유도된 myricitrin이 Phorbol-12-myristate 13-acetate (PMA)와 calcium ionophore (A23187)로 자극된 인간 비만세포(HMC-1)에서 가려움 관련 IL-31 사이토카인을 조절 하는지를 조사하는 것이었다. DLE와 myricitrin은 PMA + A23187에 의해 자극된 mRNA의 발현을 감소시키고 HMC-1 세포에서 인터루킨 IL-6, IL-31의 생성을 감소시켰다. 또한, DLE와 myricitrin은 IκB-α 인산화의 저해를 통해 핵 인자 NF-κB 활성화 및 IκB-α 인산화의 저해를 통해 핵으로의 전이를 억제하였다. HMC-1 세포에서 DLE와 myricitrin은 비만세포의 히스타민 방출을 억제하였다. DLE와 myricitrin의 이러한 약리학적 작용은 아토피성 피부염과 같은 가려움 관련 질병의 치료를 위한 기능성 물질로의 잠재적 적용 가능성을 보여주고 있다. 이 연구의 세 번째 장은 마우스에서 UVB 유발 피부 손상에 대한 DLE의 보호 효과를 관찰하고자 하였다. 마우스 피부에 UVB 조사하면 마우스 피부 조직에서 티오바르투르산 반응 물질이 증가하고, 수퍼옥사이드 디스뮤타아제와 글루타치온의 수치가 감소함으로 마우스 피부에 심각한 손상을 입힌다. 더군다나, UVB 조사는 마우스 피부에서 콜라겐이 분해되고 비만세포와 호중구가 침투되면서 염증을 야기한다. 그러나, UVB를 조사한 마우스 피부에 DLE를 처리한 결과 L-아스코르빈산과 대등하게 피부손상을 현저하게 완화시켰다. 결론적으로, 본 연구는 DLE가 산화 스트레스와 UVB 유발 피부 손상을 억제함으로써 피부 손상에 대한 국소 치료제로서의 가능성을 가지고 있음을 보여주고 있다. 이상의 결과를 요약하면, DLE와 그 유도체는 산화적 손상 및 UVB 유발성 피부 손상을 억제함으로써 산화 스트레스, 가려움증 관련 염증 및 자외선 피부 손상에 대한 중요한 천연 제품으로 가치가 있다. 따라서 DLE 및 그 유도체는 노화 방지 및 기능성 화장품 업계에서 중요한 소재로 이용될 수 있다고 사료된다. 주요어 : 고욤나무잎, 생물학적 활성, 산화 스트레스, 피부 손상, 항산화제, 항염제, 자외선 차단 Diospyros lotus (DL) commonly known as date-plum, is a widely cultivated species of the genus Diospyros, native to subtropical southwest Asia and southeast Europe, and has multiple uses in folk medicine. Various parts of this plant is used for alleviating lumbago, dysponea, hemorrhage, insomnia, and hiccup. And the leaves of DL have been used traditional tea material. It has been reported to be rich in flavonoids that have different biological activities, including antioxidant and anti-inflammatory properties. However, few studies have been reported on the antioxidant, anti-inflammatory and skin-protecting effects of DL leaf. Therefore, the purpose of this study was to investigate the biological activities D. lotus leaf extract (DLE). First section of this study was to evaluate the antioxidant properties of DLE on the chemical-induced free radical and rat red blood cell (RBC) oxidative damage in vitro. DLE showed the high antioxidant activities on the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) induced radicals. An antioxidant activities of DLE was similar to the reference antioxidant butylated hydroxytoluene and trolox. Reducing power of 1,000 ㎍/mL DLE also was similar to the vitamin C. In RBC, oxidative hemolysis induced by the aqueous peroxyl radical generator 2,2’­azobis (2-methylpropionamidine) dihydrochloride (AAPH) were significantly suppressed by DLE in a dose-dependent manner. Furthermore, DLE prevented the depletion of cytosolic antioxidant glutathione in RBC damaged with AAPH. These results suggest that DLE may has value as natural product with its high quality antioxidant properties against oxidative stress. Second section of this study was to examine whether DLE and derived myricitrin modulate itch-related IL-31 cytokines in phorbol-12 -myristate 13-acetate (PMA) plus calcium ionophore (A23187)-stimulated human mast cells (HMC-1). DLE and myricitrin decreased PMA plus A23187-stimulated mRNA expression and production of interleukin IL-6, and IL-31 in HMC-1 cells. In addition, DLE and myricitrin suppressed nuclear factor NF-κB activation and translocation to the nucleus through the inhibition of IκB-α phosphorylation. DLE and myricitrin further prevented mast cell release of histamine in HMC-1 cells. These pharmacological actions of DLE and myricitrin provide a new suggestion that shows the potential applicability of functional materials for treatment of itching-related disease such as atopic dermatitis. Third section of this study was to investigate the protective effect of DLE on Ultraviolet B radiation (UVB)-induced skin damage in mice. UVB irradiation of mice skin incurred significant damage to mice skin; increased thiobarbituric acid-reactive substance level; decreased superoxide dismutase; and glutathione levels in mice skin tissues. More so, UVB irradiation led to collagen degradation and infiltration of mast cell and neutrophils into mice skin leading to inflammation. However, topical application of DLE significantly reversed these conditions with the result comparable to L-ascorbic acid. Myricitrin, gallic acid, astragalin, myricetin-3-O-glactosside, and myricetin through high-performance liquid chromatography analysis were further determined to be the primary active compounds in DLE. In conclusion, the present study showed that DLE has potentials as local therapeutic materials against skin damage by inhibiting oxidative stress and UVB-induced skin damage. Taken together, DLE and its derivatives have value as important natural product against oxidative stress, itching-related inflammation and UV skin damage by inhibiting oxidative stress and UVB-induced skin damage. Therefore, DLE and its derivatives may use as important materials in industry of anti-aging and functional cosmetics. Key words : Diospyros lotus leaf, biological activities, oxidative stress, skin damage, antioxidant, anti-inflammatory, ultraviolet protection

나노 영가철을 이용한 국내 지하수내 질산성질소(NO3--N) 소거 활용성 조사

유선일 전주대학교 일반대학원 2017 국내석사

As a result of the survey on major domestic water pollutants in the recent five years, the average of total samples exceeding the guideline was 5.2%, among which NO3--N(Nitrate-Nitrogen) was 22.1%. The most commonly used removal method for the NO3--N was ion exchange resin so far. The research trend of the nZVI(nano-Zero Valent Iron) was conducted through keyword search during the last 10 years. The number of foreign documents was 1,410 and showed an increasing trend in every year. However, there were 33 domestic documents and difficult to find the increasing tendency. Typically, the nZVI is synthesisd through the consequence processes of mixing step, separation step, cleaning step, and drying step. However, the nZVI is so quickly oxidative, that corrosion and coagulation are happened together. Recently, additional methods such as surface modification, immobilization, dual solvent, and others have been applied to synthesis of the nZVI. In this study, the ability of nZVI prepared without washing process was compared with nZVI prepared by clean up process, including the GT-nZVI(Green Tea-nZVI) synthesized utilizing green tea. The NO3--N concentration was measured by using spectrophotometric method and the removal efficiency of NO3--N was ranged between 16% and 79%, depending on the experimental methods. The nZVI was used to remove heavy metals and ionic pollutants, especially NO3--N removal. Research has also shown that experiments involving the removal of NO3--N accounted for a large proportion. From this point of view, the nZVI will be expected to us as one of the practical ways in the future for removal of NO3--N rather than other pollutants. The ability of the nZVI to remove contaminants, synthesis and removal mechanisms of the literature are suggested. The comparison studies with existing methods were investigated for removing NO3--N from the ground water. As a result, the bio-degradable polymer of the surface modification of the nZVI was found to be the most practical method in the field. It is expected that surface deformation studies and trials for testing practical use of the nZVI will be worthy to pursue further. Keywords: nZVI, Green Tea, Nitrate-Nitrogen, Polymer, Surface modific- ation, mechanism, ground water