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Curcumin promotes oxidative stress, apoptosis and autophagy in H9c2 rat cardiomyoblasts
Iván Zepeda‑Quiróz,Helen Sánchez‑Barrera,Zaira Colín‑Val,Diana Xochiquetzal Robledo‑Cadena,Sara Rodríguez‑Enríquez,Rebeca López‑Marure 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4
Background Curcumin, a polyphenol derived from Curcuma longa, has some adverse efects on heart; however, its toxic efects on cardiac cells are poorly understood. Objective To evaluate the toxicity of curcumin on H9c2 rat cardiomyoblasts. To this, H9c2 cells were exposed to diferent concentrations of curcumin and proliferation, viability, cell cycle, oxidative stress, mitochondrial membrane potential (ΔΨm), death and autophagy were evaluated. Results Curcumin caused concentration-dependent inhibition of H9c2 cells proliferation and viability. A higher sub-G1 population was observed in cells treated with curcumin, which was related with phosphatidylserine translocation and increase of activated caspase-9, indicating apoptotic death. Curcumin induced oxidative stress and decreased ΔΨm causing mitochondrial dysfunction. Additionally, it promoted autophagy, revealed by higher LC3B and beclin-1 protein expression and mitophagy. Conclusion Curcumin exhibited toxic efects in cardiac cells and further studies are required to validate its therapeutic potential and use as anti-infammatory and anti-oxidant agent in the cardiovascular system.