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Lynn Heo(Lynn Heo),Yongmin Cho(Yongmin Cho),Junhyeok Choi(Junhyeok Choi),Jeongwook Lee(Jeongwook Lee),Yoobin Han(Yoobin Han),Sang-Wook Han(Sang-Wook Han) 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.3
Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coen-zyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyri-doxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propa-gation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identi-fied protein is a potential target for developing an effi-cient anti-virulence reagent to control BFB.