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      • Site-specific N-glycosylation identification of recombinant human lectin-like oxidized low density lipoprotein receptor-1 (LOX-1)

        Yifan Qian,Xingwang Zhang,Lei Zhou,Shifang Ren,Jianxin Gu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

        Human LOX-1/OLR 1 plays a key role in atherogenesis and endothelial dysfunction. The N-glycosylation of LOX-1 has been shown to affect its biological functions in vivo and modulate the pathogenesis of atherosclerosis. However, the N-glycosylation pattern of LOX-1 has not been described yet. The present study was aimed at elucidating the N-glycosylation of recombinanthuman LOX-1 with regard to N-glycan profile and N-glycosylation sites. Here, an approach using nonspecific protease (Pronase E) digestion followed by MALDI-QIT-TOF MS and multistage MS (MS3) analysis is explored to obtain site-specificN- glycosylation information of recombinanthuman LOX-1, in combination with glycan structure confirmation through characterizing released glycans using tandem MS. The results reveal that N-glycans structures as well as their corresponding attached site of LOX-1 can be identified simultaneously by direct MS analysis of glycopeptides from non-specific protease digestion. With this approach, one potential glycosylation site of recombinanthuman LOX-1 on Asn139 is readily identified and found to carry heterogeneous complex type N-glycans. In addition, manual annotation of multistage MS data utilizing diagnostic ions, which were found to be particularly useful in defining the structure of glycopeptides and glycans was addressed for proper spectra interpretation. The findings described herein will shed new light on further research of the structure-function relationships of LOX-1 N-glycan.

      • SCOPUSKCI등재SCIE

        Effects of fireworks on air quality in the main urban area of Nanchong City during the spring festival of 2014-2019

        Yifan Qian,Xu Yuan,Wen Dou,Jian Hu,Jie Xia,Danyu Li,Qing Zheng,Ping Zhang,Qiumei Quan,Yunxiang Li 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2

        The sources and variation trend of ambient air pollutants, the duration of pollution process and the conditions for decontamination were analyzed in this essay based on the ambient air pollutants data, PM<SUB>2.5</SUB> source analysis data and meteorological factors in Nanchong City during 2014 to 2019. The results indicated that the concentrated discharge of fireworks on New Year’s Eve was the main reason for the sharp deterioration of the ambient air quality. PM<SUB>2.5</SUB> and PM<SUB>10</SUB> concentrations increased significantly after fireworks were set off, and the peak value appeared at about 02:00 on the Lunar New Year’s Day, but the value of PM<SUB>2.5</SUB>/PM<SUB>10</SUB> did not change significantly. The concentration of SO₂ in gaseous pollutants was slightly affected by fireworks, while NO₂, CO and O₃ were not affected basically. Having been affected by fireworks, the concentrations and percentages of K⁺, Cl⁻ and Mg<SUP>2+</SUP> of the water-solubility ions in PM<SUB>2.5</SUB> significantly increased at the 0.01 level. Meanwhile, the concentrations and percentages of the metal elements, including Cu, Pb, Mg, Al, K and Ba, significantly increased at the 0.05 level. Restricted to the topography and meteorological factors, the duration of the pollution was prolonged.

      • Enhancing aircraft engine remaining useful life prediction via multiscale deep transfer learning with limited data

        LIU QIAN,Zhang Zhiyao,GUO PENG,WANG YIFAN,Liang Junxin 한국CDE학회 2024 Journal of computational design and engineering Vol.11 No.1

        Predicting the remaining useful life (RUL) of the aircraft engine based on historical data plays a pivotal role in formulating maintenance strategies and mitigating the risk of critical failures. None the less, attaining precise RUL predictions often encounters challenges due to the scarcity of historical condition monitoring data. This paper introduces a multiscale deep transfer learning framework via integrating domain adaptation principles. The framework encompasses three integral components: a feature extraction module, an encoding module, and an RUL prediction module. During pre-training phase, the framework leverages a multiscale convolutional neural network to extract distinctive features from data across varying scales. The ensuing parameter transfer adopts a domain adaptation strategy centered around maximum mean discrepancy. This method efficiently facilitates the acquisition of domain-invariant features from the source and target domains. The refined domain adaptation Transformer-based multiscale convolutional neural network model exhibits enhanced suitability for predicting RUL in the target domain under the condition of limited samples. Experiments on the C-MAPSS dataset have shown that the proposed method significantly outperforms state-of-the-art methods.

      • SCOPUSKCI등재

        Feature analysis on air quality in the main urban area of Nanchong City in 2015–2018

        Xiong Lei,Qing Zheng,Yifan Qian,Jian Hu,Danyu Li,Ping Zhang,Xu Yuan,Qiumei Quan,Yunxiang Li 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3

        This study evaluates the air quality in Nanchong City in 2015–2018 based on automatic monitoring data and meteorological data in the main urban area. Results show that overall air quality in the main urban area of Nanchong was standard and improved from 2015 to 2018. SO₂, NO₂, and CO had slight influences on air quality in the study area, whereas excessive annual average PM2.5 and increasing O₃ concentration challenged the air quality. The monthly average mass concentrations of particulate matters (PM2.5 and PM10), NO₂, and CO generally presented a “U-shaped” distribution pattern while O₃ showed the opposite. The diurnal variation of PM, NO₂, and CO has two peaks related to the morning and evening rush hours. Vehicle exhaust is the main source of local pollution. Over-standard PM2.5 can significantly inhibit O₃ production while O₃ can easily promote the secondary generation of PM2.5 due to its strong oxidization. PM2.5 emission should be controlled strictly in winter, while in summer, the sources of O₃ should be controlled vigorously. Air pollution is comprehensive and complicated and is influenced by various factors, mainly meteorological and human factors. Measures such as artificial rainfall should be included at the appropriate time to cope with adverse meteorological conditions when heavy pollution occurs in winter.

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