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KIM, Daekyung,YAMASAKI, Yasuhiro,YAMATOGI, Toshifumi,YAMAGUCHI, Kenichi,MATSUYAMA, Yukihiko,KANG, Yang-Soon,LEE, Yoon,ODA, Tatsuya Japan Society for Bioscience, Biotechnology, and A 2009 Bioscience, Biotechnology, and Biochemistry Vol.73 No.3
<P>To elucidate the ichthyotoxic mechanism of the harmful dinoflagellate <I>Cochlodinium polykrikoides</I>, a bioassay using damselfish was conducted. After exposure to a live-cell suspension of <I>C. polykrikoides</I>, all the fish were died within 90 min. In the presence of catalase and superoxide dismutase (SOD), no significant reduction in the toxicity of <I>C. polykrikoides</I> on the fish was observed. Furthermore, no significant levels of reactive oxygen species (ROS) were detected in five strains of <I>C. polykrikoides</I> isolated in Japan. Our results support the idea that certain toxic substances, rather than ROS, are mainly responsible for the fish-killing activity of <I>C. polykrikoides</I>.</P>