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RISS 인기검색어
- 검색키워드
- 저자 : Uzzal Somaddar (검색결과 2 건)
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Hafsa Sultana,Uzzal Somaddar,Swadesh Chandra Samanta,Abul Kashem Chowdhury,Gopal Saha 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.2
Development of salt tolerance in rice through breeding program is mainly depends on the salinity responses of thepotential rice germplasms. Coastal rice landraces of Bangladesh possess diverse morphological and physiological responses to salinity. Hence, our target is to identify candidate salt-tolerant coastal rice genotypes as a new source of salt tolerance (12 dS/m). Here, weannotated 20 Bangladeshi coastal Aus landrace rice regarding their phenotypic and genetic relatedness to salinity tolerance throughmultivariate analyses of five morpho-physiological traits namely, salt injury score (SIS), ion-leakage, chlorophyll concentration,root-shoot reduction percentage and profiling of DNA using simple sequence repeat (SSR). Based on the standard evaluation score(SES) the salt-induced coastal rice landraces were grouped into highly susceptible (HS), susceptible (S), moderately tolerant (MT),tolerant (T) and highly tolerant (HT). Besides, a canonical discrimination analysis of the mean trait values of five morpho-physiologicalparameters confirmed the above mentioned five categories of salinity tolerance. Based on all morpho-physiological parameters onegenotype (Kalihytta) as highly tolerant (HT), two genotypes (Manikmuri and Monsur IRRI) as tolerant (T) and five genotypes (Nara,Iratom 27, Matichak, Abdul high IRRI and Parija) were identified as moderately tolerant (MT) against salinity. Finally, the molecularcharacterization using two SSR markers (RM493 and RM3412) revealed Kalihytta, Nara, Iratom 27, Parija, Lal jamaibabu andFullbadam, as tolerant against salt stress. Our candidate salt tolerant Aus rice genotypes could be useful as novel sources of salttolerance for thriving salt-tolerant high yielding varieties in the coastal ecosystem of Bangladesh.
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Yeo-Hyeon Kim,Sopheap Mao,Nihar Sahu,Uzzal Somaddar,Hoy-Taek Kim,Masao Watanabe,박종인 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.5
Xanthomonas campestris pv. campestris (Xcc) is a plant pathogen of Brassica crops that causes black rot disease throughout the world. At present, 11 physiological races of Xcc (races 1-11) have been reported. The conventional method of using differential cultivars for Xcc race detection is not accurate and it is laborious and time-consuming. Therefore, the development of specific molecular markers has been used as a substitute tool because it offers an accurate and reliable result, particularly a quick diagnosis of Xcc races. Previously, our laboratory has successfully developed race-specific molecular markers for Xcc races 1-6. In this study, specific molecular markers to identify Xcc race 7 have been developed. In the course of study, whole genome sequences of several Xcc races, X. campestris pv. incanae, X. campestris pv. raphani, and X. campestris pv. vesicatoria were aligned to identify variable regions like sequence-characterized amplified regions and insertions and deletions specific to race 7. Primer pairs were designed targeting these regions and validated against 22 samples. The polymerase chain reaction analysis revealed that three primer pairs specifically amplified the DNA fragment corresponding to race 7. The obtained finding clearly demonstrates the efficiency of the newly developed markers in accurately detecting Xcc race 7 among the other races. These results indicated that the newly developed marker can successfully and rapidly detect Xcc race 7 from other races. This study represents the first report on the successful development of specific molecular markers for Xcc race 7.
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