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Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphisms of the CYP2E1 Gene
Suhda, Saihas,Paramita, Dewi Kartikawati,Fachiroh, Jajah Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.7
Single nucleotide polymorphism (SNP) detection has been used extensively for genetic association studies of diseases including cancer. For mass, yet accurate and more economic SNP detection we have optimized tetra primer amplification refractory mutation system polymerase chain reaction (ARMS PCR) to detect three SNPs in the cytochrome P450 2E1 (CYP2E1) gene locus; i.e. rs3813865, rs2070672 and rs3813867. The optimization system strategies used were (1) designing inner and outer primers; (2) determining of their optimum primer concentration ratios; and (3) determining of the optimum PCR annealing temperature. The tetra primer ARMS PCR result could be directly observed using agarose gel electrophoresis. The method succesfully determined three SNPs in CYP2E1 locus, the results being consistent with validation using DNA sequencing and restriction fragment length polymorphisms (RFLP).
A New Winter-Type Malting Barley Cultivar, "Dajin" with Powdery Mildew and BaYMV Resistance
Jong Nae Hyun,Sae Jung Suh,Hyun Tae Kim,Seong Bum Baek,Sang Ik Han,Jong Rae Kang,Jong Min Ko,Sea Gye Lim,Jong Chul Park,Mi Jung Kim,Jung Gon Kim,Jung Hyun Nam,Duck Yong SuhDa 한국육종학회 2006 한국육종학회지 Vol.38 No.1
Suwon295 at the Honam Agricultural Research Institute (HARI) in 2005. An elite line, 953017-BG-BN-BN-53N, was selected in2000 and designated as Milyang126. It showed good agronomic performance in the regional adaptation yield trials (RYT) from2003 to 2005