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RISS 인기검색어
- 검색키워드
- 저자 : Snehal Nitin Mestry (검색결과 2 건)
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Snehal Nitin Mestry,Archana Ramesh Juvekar 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.3
Cataract is one of the primitive secondary complications of diabetes mellitus and contributes to 50% blindness worldwide. Aldose reductase (AR) is the first and rate limiting enzyme in the intracellular polyol pathway responsible for the development of cataract. Thus, there is an imperative need for modest, non-surgical way in order to treat cataract. In Ayurveda, Punica granatum (Pomegranate) is considered as “a pharmacy unto itself”. Pomegranate leaves are traditionally used for their anti-diabetic activity. In this study, we investigated AR inhibitory activity and anti-cataract potential of Pomegranate leaves in glucose - induced cataract in goat lens. AR inhibitory activity was evaluated in vitro in goat lenses. In glucose induced cataract model, goat lenses were incubated in artificial aqueous humor containing 55 mM glucose along with methanolic extract of Punica granatum leaves (MPGL) or quercetin for 72 h. After 72 h, photographic evaluation of the lens was carried out and the lens antioxidant parameters were determined. Different concentrations of MPGL were found to inhibit lens AR activity with an IC50 value of 83.55 ± 3.92 μg/ml. Quercetin was taken as positive control which exhibited 56.461% AR inhibition at a concentration of 500 μg/ml. Cataractous lenses exhibited marked opacity and decrease in the levels of reduced glutathione and superoxide dismutase. Treatment with MPGL and quercetin prevented the opacity and restored back the antioxidant activity. Together, Punica granatum leaves ameliorated glucose-induced cataractogenesis via AR inhibition, reduction in oxidative stress and improvement in antioxidant defense system.
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Snehal Nitin Mestry,Nitin Bhanudas Gawali,Malvika Sunil Gursahani,Sarayu Arvind Pai,Jayesh Bachu Dhodi,Archana Ramesh Juvekar 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.2
Advanced glycation end products (AGEs) are the consequent products by virtue of non-enzymatic reaction of reducing sugars with amino groups present either in lipids, nucleic acids or proteins. Lately AGEs have been embroiled in the inception of diabetes induced complications. In Ayurveda, Punica granatum is contemplated as “a pharmacy unto itself”. Punica granatum leaves have been stated to have anti-diabetic activity. The purpose of the existing study was to determine the anti-glycation potential of methanolic extract of Punica granatum leaves (MPGL) and investigate the fundamental mechanisms in discouraging the glycation process. Antioxidant activity of MPGL was estimated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (3-ethyl-benzothiazoline-6-sulfonic acid) radical scavenging assays. Both MPGL and aminoguanidine were evaluated against the formation of AGEs. Antiglycation activity was evaluated by determining the fluorescence intensity of AGEs and formation of fructosamine. Protein oxidation in the glycation process was determined by evaluating the protein carbonyl content and thiol group. MPGL demonstrated IC50 value of 15.602 ± 0.296 and 724.323 ± 16.451 µg/ml for the DPPH and ABTS radical scavenging assays respectively. Current study revealed that MPGL had cogent effects on the in vitro formation of AGEs. MPGL decreased fructosamine level and significantly counteracted the oxidative damage of protein by curbing protein carbonyl content and impeding the loss of thiol group. The anti-glycation activity was farther cogent than the glycation inhibitory activity attained using the standard drug, aminoguanidine. Thereupon, MPGL presents to be more encouraging to be emerged as an competent AGE inhibitor.
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