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RISS 인기검색어
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- 저자 : Shiqiang Wu (검색결과 3 건)
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Purification and Characteristics of Serine Protease from the Head of Pacific White Shrimp
Yanyan Wu,Ping Wang,Laihao Li,Xianqing Yang,Shiqiang Diao 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.4
Serine protease from the head of Pacific white shrimp was purified by the following techniques: ammonium sulfate fractionation, Q-Sepharose HP ion exchange chromatography, and Sephadex G-100 gel filtration. The molecular weight was estimated as 32.8 kDa using SDSPAGE. The optimum pH and temperature of the enzyme for the hydrolysis of casein were determined to be 10.0 and 40oC. It was stable at pH range from 8.0 to 11.0 and had good thermal stability. Pb2+, Ca2+, Mg2+, Cu2+, and Mn2+could active the enzyme certainly when Zn2+and Hg2+strongly inhibited the activity. The enzyme was inhibited by the general serine protease inhibitor (PMSF) and the specific trypsin inhibitors (TLCK, SBTI). The modification of various amino acid modifiers for the purified enzyme determined that the enzyme active center included tryptophan,histidine, and serine, moreover, arginine had a certain relationship with the enzyme activity.
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The behaviors of LNAPL migration and entrapment in a rough-walled vertical fracture
Bin Lu,Shiqiang Wu,Xinghua Xie,Yefei Tan 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.3
The migration of light non-aqueous phase liquids (LNAPLs) and their entrapment in unsaturated vertical fractures exhibit complex behaviors, such as upward LNAPL migration that follows water level rise. In this study, behavior of LNAPL migration and entrapment in a vertical rough-walled fracture was investigated with optical measurement. A series of experiments were preformed, including fracture drainage, LNAPL infiltration and accumulation in an unsaturated fracture, and upward LNAPL migration with water level rise. Experimental results indicate that the fracture aperture significantly influences the LNAPL migration path and entrapped blobs distribution. The LNAPL-filled aperture follows a normal distribution, with the mean aperture ranging 0.253 mm to 0.287 mm, and the standard deviation around 0.13 mm. The characteristics of the entrapment blob morphology indicate that 80% of the individual blob areas are smaller than 20mm2. The irregular structures of discrete blobs increase the ‘LNAPL-water’ interface, which will exacerbate contamination.
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Stability-based On-demand Multi-path Distance Vector Protocol for Edge Internet of Things
Dongzhi Cao,Peng Liang,Tongjuan Wu,Shiqiang Zhang,Zhenhu Ning 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.10
In edge computing scenarios, IoT end devices play a crucial role in relaying and forwarding data to significantly improve IoT network performance. However, traditional routing mechanisms are not applicable to this scenario due to differences in network size and environment. Therefore, it becomes crucial to establish an effective and reliable data transmission path to ensure secure communication between devices. In this paper, we propose a trusted path selection strategy that comprehensively considers multiple attributes, such as link stability and edge cooperation, and selects a stable and secure data transmission path based on the link life cycle, energy level, trust level, and authentication status. In addition, we propose the Stability-based On-demand Multipath Distance Vector (STAOMDV) protocol based on the Ad hoc AOMDV protocol. The STAOMDV protocol implements the collection and updating of link stability attributes during the route discovery and maintenance process. By integrating the STAOMDV protocol with the proposed path selection strategy, a dependable and efficient routing mechanism is established for IoT networks in edge computing scenarios. Simulation results validate that the proposed STAOMDV model achieves a balance in network energy consumption and extends the overall network lifespan.
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