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Shinde, P.B.,Han, A.R.,Cho, J.,Lee, S.R.,Ban, Y.H.,Yoo, Y.J.,Kim, E.J.,Kim, E.,Song, M.C.,Park, J.W.,Lee, D.G.,Yoon, Y.J. Elsevier Science Publishers 2013 Journal of biotechnology Vol.168 No.2
Expression plasmids carrying different deoxysugar biosynthetic gene cassettes and the gene encoding a substrate-flexible glycosyltransferase DesVII were constructed and introduced into Streptomyces venezuelae YJ003 mutant strain bearing a deletion of a desosamine biosynthetic (des) gene cluster. The resulting recombinants produced macrolide antibiotic YC-17 analogs possessing unnatural sugars replacing native d-desosamine. These metabolites were isolated and further purified using chromatographic techniques and their structures were determined as d-quinovosyl-10-deoxymethynolide, l-rhamnosyl-10-deoxymethynolide, l-olivosyl-10-deoxymethynolide, and d-boivinosyl-10-deoxymethynolide on the basis of 1D and 2D NMR and MS analyses and the stereochemistry of sugars was confirmed using coupling constant values and NOE correlations. Their antibacterial activities were evaluated in vitro against erythromycin-susceptible and -resistant Enterococcus faecium and Staphylococcus aureus. Substitution with l-rhamnose displayed better antibacterial activity than parent compound YC-17 containing native sugar d-desosamine. The present study on relationships between chemical structures and antibacterial activities could be useful in generation of novel advanced antibiotics utilizing combinatorial biosynthesis approach.
Shinde, Pravin S.,Annamalai, Alagappan,Kim, Jae Young,Choi, Sun Hee,Lee, Jae Sung,Jang, Jum Suk American Chemical Society 2015 The Journal of Physical Chemistry Part C Vol.119 No.10
<P>High:quality hematite (alpha-Fe2O3) photoanodes were syritheSized from a sulfate electrolyte bath by the pulse reverse electrodeposition (PRED) method. The influence of PRED parameters (viz, duty cycle, pulse period, and deposition time) was systematically investigated on the structural, optical, morphological, and photo electrochemical properties of the films. The optimized parameters of pulse duty cycle, pulse:period, and the deposition time were 20%, 10 ms, and 45 s, respectively. The granular and compact nanocrystalline morphology of the alpha-Fe2O3 was found to alter according to the process parameters. The alpha-Fe2O3 electrodes (film thickness similar to 200 nm) prepared by annealing at 550 degrees C for 4 h followed by 800 degrees C for 15 min exhibited an optimum photocurrent density of 504 mu A cm(-2) measured at 1.23 V vs RHE in 1 M NaOH electrolyte under 100 mW cm(-2) light illumination.</P>
Shinde, S. S.,Sami, Abdul,Lee, Jung-Ho The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.24
<P>Electrocatalytic hydrogen evolution using non-precious metals or metal-free catalysts is critically necessary because platinum-based electrocatalysts are greatly limited in scalable commercialization of hydrogen generation due to their high cost. Here, we report the facile synthesis of metal-free hybrid catalysts, in which graphitic carbon nitride (g-C3N4) is coupled with nanoporous graphene doped by S and Se. The S and Se co-doped hybrid catalyst (g-C3N4@S-Se-pGr) reveals superior electrocatalytic performances, including an exchange current density of 6.27 × 10<SUP>−6</SUP>A cm<SUP>−2</SUP>, an on-set potential of 0.092 V, a Tafel slope of 86 mV dec<SUP>−1</SUP>, an adsorption free energy of −0.13 eV, and long-term stability comparable to those of commercial Pt/C catalysts. Volcano plots showing the hydrogen evolution activity<I>versus</I>adsorption free energy are also compatible with those of the conventional metal catalysts. Our strategy has the potential to allow a new paradigm for the development of high-performance metal-free electrocatalysts for energy conversion devices.</P>
Control of rhGH Release Profile from PEG–PAF Thermogel
Shinde, Usha Pramod,Moon, Hyo Jung,Ko, Du Young,Jung, Bo Kyong,Jeong, Byeongmoon American Chemical Society 2015 Biomacromolecules Vol.16 No.5
<P>Poly(ethylene glycol)-poly(<SMALL>l</SMALL>-alanine-<I>co</I>-<SMALL>l</SMALL>-phenyl alanine) diblock copolymers (PEG–PAF) of 2000–990 Da (P2K) and 5000–2530 Da (P5K) with the different molecular weights of PEGs, but having a similar molecular weight ratio of hydrophobic block to hydrophilic block were synthesized to compare their solution behavior and corresponding protein drug release profiles from their in situ formed thermogels. The PEG–PAF aqueous solutions underwent heat-induced sol-to-gel transition in a concentration range of 18.0–24.0 wt % and 8.0–12.0 wt % for P2K and P5K, respectively. P5K formed bigger micelles than P2K, of a broad distribution, whereas the PAF blocks of P5K developed richer in α-helix than those of P2K in the core of the micelles. As the temperature increased, the micelles underwent dehydration of the PEG, which led to the aggregation of micelles, while the secondary structure of PAF was slightly affected during the sol-to-gel transition. The P5K exhibited higher tendency to aggregate and formed a tighter gel than P2K. Upon injection into the subcutaneous layer of rats, both polymer aqueous solutions formed a biocompatible gel with typical mild inflammatory tissue responses. Recombinant human growth hormone (rhGH) maintained its stability without forming any aggregates in both sol (4 °C) and gel (37 °C) states of the PEG–PAFs. Even though P2K and P5K have a similar molecular weight ratio of hydrophobic block to hydrophilic block, the P5K system exhibited a reduced initial burst release, improved bioavailability, and prolonged therapeutic duration of the rhGH, compared to the P2K system. The current research suggests that a drug release profile is a complex function of self-assembling carriers and incorporated drugs, and thus, a promising protein delivery system could be designed by adjusting the molecular parameters of a thermogel.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2015/bomaf6.2015.16.issue-5/acs.biomac.5b00325/production/images/medium/bm-2015-00325x_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm5b00325'>ACS Electronic Supporting Info</A></P>
Shinde, Pravin S.,Go, Geun Ho,Lee, Won Jae The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.21
<P>Nanocrystalline hematite (α-Fe<SUB>2</SUB>O<SUB>3</SUB>) film was prepared on FTO using pulse reverse electrodeposition with hierarchical nano-petal structures (∼30 to 35 nm) that appear to protrude out from the agglomerated network. Photocurrent density of nano-petal morphology measured at <I>E</I> = 1.23 V <I>vs.</I> RHE in 1 M NaOH under 1 sun illumination was remarkably increased by 180% relative to that of Fe<SUB>2</SUB>O<SUB>3</SUB> film produced by normal electrodeposition.</P> <P>Graphic Abstract</P><P>Nanocrystalline hematite (α-Fe<SUB>2</SUB>O<SUB>3</SUB>) film was prepared on FTO using pulse reverse electrodeposition with hierarchical nano-petal structures (∼30 to 35 nm) that appear to protrude out from the agglomerated network. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm31254a'> </P>
Shinde, Dipak V.,Mane, Rajaram S.,Oh, In-Hwan,Lee, Joong Kee,Han, Sung-Hwan The Royal Society of Chemistry 2012 Dalton Transactions Vol.41 No.34
<P>A novel architecture of SnO<SUB>2</SUB> nanowall-arrays coated with rutile-TiO<SUB>2</SUB> nanoneedles is fabricated for the first time and envisaged in dye-sensitized solar cells. Devices constructed using these architectures showed a power conversion efficiency of 4.12%, which is the highest among the SnO<SUB>2</SUB> nanostructures grown on conducting substrates by wet chemical methods.</P> <P>Graphic Abstract</P><P>Figure shows a false color scanning electron microscope image of SnO<SUB>2</SUB>–TiO<SUB>2</SUB> architectures and current–voltage characteristics of the device sensitized with N719 dye under 1 Sun illumination. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2dt31317k'> </P>
Shinde, Vijay Vilas,Jeong, Yeon Tae The Royal Society of Chemistry 2015 NEW JOURNAL OF CHEMISTRY Vol.39 No.6
<P>A one-pot multi-component, green, and highly efficient protocol has been developed for the synthesis of imidazo[1,2-a] pyrimidines annulated heterocyclic scaffolds (4a-4ab) using molybdate sulfuric acid (MSA) as an eco-friendly and reusable catalyst in good to excellent yields under solvent-free conditions that efficiently produced three new bonds (two C-N and one C-C) in a single operation. Other remarkable features of this environmentally benign protocol are a short reaction time, a wide range of functional group tolerance, use of an inexpensive heterogeneous catalyst, and high yield of products via a simple experimental and work-up procedure. The catalyst can be recovered and reused for at least four runs without any significant impact on the product yields. [GRAPHICS] .</P>