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Jae-Hong Lim,Seung-Jun Seo,Hyo-Yun Kim,Chun Kil Ryu,Seungyu Rah,Jung Yun Huang,Chae-Soon Lee,In Deuk Seo,Seob-Gu Kim,Dongyun Lee,Moo-Hyun Cho 한국구조생물학회 2017 Biodesign Vol.5 No.2
Similar to macromolecular crystallography (MX), radiography uses X-rays to reveal structures, however, of macro objects, which can be several orders of magnitude larger in size than the subjects of MX. Beamline 6C Bio Medical Imaging of the Pohang Light Source-II (PLS-II) utilizes synchrotron radiation X-rays to implement parallel beam projection radiography. Major applications are virtual anatomy of small animals and plants, non-invasive physiological monitoring and 3D histology in biology and medicine. Uses in material science and defect analysis of industrial products are also becoming popular. In this review, the use of the beamline is introduced including sample preparation, data acquisition and a workflow for 3D image reconstruction, visualization and analysis. For biologists who are familiar with MX, a brief comparison has been made between the two techniques. The mechanisms for spatial resolution determination and contrast formation in the parallel beam projection radiography are also presented.
Small-angle X-ray Scattering Beamline BL4C SAXS at Pohang Light Source II
Kwang-Woo Kim,Jehan Kim,Young Duck Yun,Hyungju Ahn,Byoungseok Min,Na Hyung Kim,Seungyu Rah,Hyo-Yun Kim,Chae-Soon Lee,In Deuk Seo,Woul-Woo Lee,Hyeong Joo Choi,Kyeong Sik Jin 한국구조생물학회 2017 Biodesign Vol.5 No.1
BL4C SAXS at the Pohang Light Source II is a small-angle X-ray scattering beamline based on an in-vacuum undulator insertion device, Si(111) DCM, and toroidal focusing mirror. The beamline normally provides high-flux synchrotron radiation X-ray sources with energies from 10.3 to 20.6 keV and a 100 µm (vertical) × 300 µm (horizontal) full width at half-maximum focal spot. The analysis of the SAXS data would be facilitated by means of useful ancillary equipment. The design of the beamline, the key components, and its role are described.
Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution
Kim, Kyong Woo,Kwon, Youngman,Nam, Ki-Yong,Lim, Jong-Hyeok,Kim, Kyu-Gyum,Chon, Kwon Su,Kim, Byoung Hoon,Kim, Dong Eon,Kim, JinGon,Ahn, Byoung Nam,Shin, Hyun Joon,Rah, Seungyu,Kim, Ki-Ho,Chae, Jin Seok Institute of Physics in association with the Ameri 2006 Physics in medicine & biology Vol.51 No.6
<P>In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at λ = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at ?580 magnification and 1 min exposure. Images of a biological sample (<I>Coscinodiscus oculoides</I>) were recorded.</P>