http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
눈알고둥,Lunella coronata coreensisd의 생식주기
오성립,나오수,이영돈,김형배 제주대학교 해양연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-
We investigated reproductive cycle of the turban shell. Lunella coronata coreensis with the histological preparation of gonads and the gonadosomatic index (GSI). The turban shells were collected at the intertidal zone of Hamdeok in Jeju-Do from December. 1995 to October. 1996. The gonad was located on the surface of the liver below the stomachal caecum Posterior spiral meat part of the shell. GSI value began to increase from May as water temperature increased and reached its maximum value in August both male and female which were 56.94 and 61.88. respectively. GSI started to decrease from September thereafter. maintaining relatively low value from January to March. The reproductive cycle of L. coronata coreensis could be grouped into five successive developmental stages : multiplicative stage (female: February to April. male. April to May). growing stage (female: April to June, male: June). mature stage (female: June to August, male: July to August). ripe and spawning stave (August to September). And degenerative and recovery stage (October to March). The spawning of the turban shell occurred from August to September and the main spawning period appeared in September. The turban shell. L. coronata coreensis appeared to be gonochoristic species. Sex ratio of female to male was 1.6 : 1.0 (P > 0.05).
Sex Reversal and Masculinization according to Growth in Longtooth Grouper Epinephelus bruneus
Seong-Rip Oh,Hyeong-Cheol Kang,이치훈,허상우,이영돈 한국발생생물학회 2013 발생과 생식 Vol.17 No.2
To understand the sex reversal characteristics in the longtooth grouper (Epinephelus bruneus), this study examined the sex reversal and artificial masculinization of wild caught E. bruneus reared in indoor rearing tank after a 17α-methyltestosterone injection. To domesticate a broodstock, 64 wild caught E. bruneus, between 47.0 to 110.0 cm in total length and from 1.5 to 21.4 kg in body weight, were reared in indoor rearing tank (4.0 to 5.0 m wide, and 2.5 to 3.0 m depth) for four years. Seven specimens showed sex reversal from female to male during indoor rearing condition,whose total length and body weights were from 63.0 to 99.0 cm and from 4.4 to 13.2 kg, respectively. After inducing artificial masculinization in 14 female E. bruneus with a 17α-methyltestosterone (2.0 mg/kg BW) implants for 3 years,spermiation occurred in 9 specimens (total length: 54.0 to 68.0 cm, body weight: 2.3 to 4.3 kg). Among the female to male sex reversals, two specimens returned back to being female, whose body weights were 2.8 kg (initially 2.6 kg) and 2.7 kg (initially 2.3 kg). Therefore, this study suggested that E. bruneus (> 3.0 kg) was more effective in masculinizing by 17α-methyltestosterone implants.
Oh, Seong-Rip,Lee, Chi-Hoon,Kang, Hyeong-Cheol,Song, Young-Bo,Kim, Hyung-Bae,Lee, Young-Don The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.4
This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: $99.5{\pm}0.8%$, DMSO 7.5%: $99.5{\pm}0.7%$, and DMSO 10.0%: $99.6{\pm}0.6%$). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: $96.2{\pm}2.3%$, DMSO 7.5%: $95.3{\pm}3.6%$, 10.0%: $96.6{\pm}1.8%$) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm ($98.4%{\pm}0.5$) and DMSO concentration level of 5.0% ($97.8{\pm}0.1%$) were not significantly different. However, with 7.5% ($97.2{\pm}0.6%$) and 10.0% DMSO concentrations ($95.9{\pm}0.2%$) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; $66.8{\pm}1.8%$, HR: $82.0{\pm}12.9%$) and 2 years (FR; $78.5{\pm}14.8%$, HR: $79.3{\pm}0.6%$) cryopreserved sperm were lower than control (FR; 100%, HR: $91.1{\pm}3.6%$) and 2 days cryopreserved sperm (FR; $99.6{\pm}0.6%$, HR: $96.6{\pm}1.8%$). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.
Sex Reversal and Masculinization according to Growth in Longtooth Grouper Epinephelus bruneus
Oh, Seong-Rip,Kang, Hyeong-Cheol,Lee, Chi-Hoon,Hur, Sang-Woo,Lee, Young-Don The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.2
To understand the sex reversal characteristics in the longtooth grouper (Epinephelus bruneus), this study examined the sex reversal and artificial masculinization of wild caught E. bruneus reared in indoor rearing tank after a 17${\alpha}$-methyltestosterone injection. To domesticate a broodstock, 64 wild caught E. bruneus, between 47.0 to 110.0 cm in total length and from 1.5 to 21.4 kg in body weight, were reared in indoor rearing tank (4.0 to 5.0 m wide, and 2.5 to 3.0 m depth) for four years. Seven specimens showed sex reversal from female to male during indoor rearing condition, whose total length and body weights were from 63.0 to 99.0 cm and from 4.4 to 13.2 kg, respectively. After inducing artificial masculinization in 14 female E. bruneus with a 17${\alpha}$-methyltestosterone (2.0 mg/kg BW) implants for 3 years, spermiation occurred in 9 specimens (total length: 54.0 to 68.0 cm, body weight: 2.3 to 4.3 kg). Among the female to male sex reversals, two specimens returned back to being female, whose body weights were 2.8 kg (initially 2.6 kg) and 2.7 kg (initially 2.3 kg). Therefore, this study suggested that E. bruneus (> 3.0 kg) was more effective in masculinizing by 17${\alpha}$-methyltestosterone implants.
Bisphenol A 가 송사리 , Oryzias latipes 의 수정란 부화와 어미의 산란에 미치는 영향
나오수(Oh Soo Na),백혜자(Hea Ja Baek),이영돈(Young Don Lee),오성립(Seong Rip Oh),김형배(Hyung Bae Kim) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.5
내분비장애물질로 알려진 BPA가 송사리 수정란의 부화와 어미의 산란량에 미치는 영향을 번식생물학적으로 조사하였다. 송사리 수정란의 부화 소요시간은 대조구 I과 II에서 9∼12일, BPA 50㎍/ℓ 처리구에서 26∼35일, BPA 100㎍/ℓ처리구에서 26∼27일 이었으나, 200㎍/ℓ 처리구에서는 부화하지 않았다. 부화율은 대조구 I과 II에서 각각 89.8, 84.3%인데 비해, BPA 50과 100㎍/ℓ 처리구에서 각각 23.3, 22.2%로 현저하게 낮았다. 송사리 어미가 산란한 알의 수는 대조구 I과 II 그리고 BPA 50㎍/ℓ body weight 처리구에서 각각 52.5, 4.70, 48.0개인데 비해, BPA 100과, 200㎍/ℓ body weight 처리구에서 각각 21.5, 18.5개로 상대적으로 산란량이 적었다. 산란한 모든 알 중에서 비정상란의 비율은 대조구 I과 II에서 각각 11.8, 16.2% 이었고, BPA 50, 100 그리고 200㎍/ℓbody weight 처리구에서 각각 38.8, 74.1%의 비율을 보여 BPA 처리 농도가 높을수록 비정상란이 많았다. 그리고 정상란의 부화율은 대조구에서 평균 78% 이상인데 비해, BPA 처리구에서 평균 50% 이하로 대조구에 비해 상대적으로 낮았다. 이들 결과들에서 BPA 처리구는 대조구에 비해 송사리의 수정란 부화와 송사리 어미의 산란량과 난질 그리고 수정에 저해를 주는 것으로 보인다. The effects of bisphenol A (BPA), known as one of the endocrine disrupting chemicals (EDCs), on the hatching of fertilized eggs (FE) and the spawning of adult fish in songsari, Oryzias latipes were investigated. In condition of the experimental groups, incubated water temperature was 25.0±5℃ and photoperiod was 16 h (light) : 8 h (dark). FE were exposed to aqueous solutions of BPA at nominal concentration of 50, 100 and 200㎍/ℓ. The time required in hatching of FE was long in the BPA 50 and 100㎍/ℓ treatment groups when compared to the controls, and in the BPA 200㎍/ℓ treatment group, FE failed to hatch. And also hatching rate was decreased in the BPA treatment groups in comparison to the controls. Adult fishes were reared to oral administration via a diet of 50, 100 and 200㎍/ℓ body weight BPA concentration for 3 weeks. Number of total eggs spawned in the adult fish were fewer in the BPA 100 and 200 treatment groups than in the controls and BPA 50 treatment group. Frequency of abnormal eggs in the total eggs spawned was 11.8 and 16.2% in the control and acetone carrier control lower than 36.8, 46.8 and 74.1% in the BPA 50, 100 and 200 treatment groups, respectively. And hatching rate of FE decreased in the BPA treatment groups in comparison to the controls. In these results, inhibition of the hatching of FE and the quantity and quality of spawned eggs in adult fish were observed from BPA treatment groups when compared to the controls.